Probiotic Potential of a Novel Vitamin B2-Overproducing <i>Lactobacillus plantarum</i> Strain, HY7715, Isolated from Kimchi
Vitamin B2, also known as riboflavin, is essential for maintaining human health. The purpose of this study was to isolate novel lactic acid bacteria that overproduce vitamin B2 and to validate their potential as probiotics. In this study, <i>Lactobacillus plantarum</i> HY7715 (HY7715) wa...
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MDPI AG
2021-06-01
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| author | Joo-Yun Kim Eun-Jung Choi Jae-Ho Lee Myeong-Seok Yoo Keon Heo Jae-Jung Shim Jung-Lyoul Lee |
| author_facet | Joo-Yun Kim Eun-Jung Choi Jae-Ho Lee Myeong-Seok Yoo Keon Heo Jae-Jung Shim Jung-Lyoul Lee |
| author_sort | Joo-Yun Kim |
| collection | DOAJ |
| description | Vitamin B2, also known as riboflavin, is essential for maintaining human health. The purpose of this study was to isolate novel lactic acid bacteria that overproduce vitamin B2 and to validate their potential as probiotics. In this study, <i>Lactobacillus plantarum</i> HY7715 (HY7715) was selected among lactic acid bacteria isolated from Kimchi. HY7715 showed a very high riboflavin-producing ability compared to the control strain due to the high expression of <i>ribA</i>, <i>ribB</i>, <i>ribC</i>, <i>ribH</i>, and <i>ribG</i> genes. HY7715 produced 34.5 ± 2.41 mg/L of riboflavin for 24 h without consuming riboflavin in the medium under optimal growth conditions. It was able to produce riboflavin in an in vitro model of the intestinal environment. In addition, when riboflavin deficiency was induced in mice through nutritional restriction, higher levels of riboflavin were detected in plasma and urine in the HY7715 administration group than in the control group. HY7715 showed high survival rate in simulated gastrointestinal conditions and had antibiotic resistance below the cutoff MIC value suggested by the European Food Safety Authority; moreover, it did not cause hemolysis. In conclusion, HY7715 could be considered a beneficial probiotic strain for human and animal applications, suggesting that it could be a new alternative to address riboflavin deficiency. |
| first_indexed | 2024-03-10T10:12:04Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T10:12:04Z |
| publishDate | 2021-06-01 |
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| series | Applied Sciences |
| spelling | doaj.art-ce8d2c41568246bf8207883b731df66c2023-11-22T01:07:44ZengMDPI AGApplied Sciences2076-34172021-06-011113576510.3390/app11135765Probiotic Potential of a Novel Vitamin B2-Overproducing <i>Lactobacillus plantarum</i> Strain, HY7715, Isolated from KimchiJoo-Yun Kim0Eun-Jung Choi1Jae-Ho Lee2Myeong-Seok Yoo3Keon Heo4Jae-Jung Shim5Jung-Lyoul Lee6R & BD Center, hy Co., Ltd., 22, Giheungdanji-ro 24beon-gil, Giheung-gu, Yongin-si 17086, KoreaR & BD Center, hy Co., Ltd., 22, Giheungdanji-ro 24beon-gil, Giheung-gu, Yongin-si 17086, KoreaR & BD Center, hy Co., Ltd., 22, Giheungdanji-ro 24beon-gil, Giheung-gu, Yongin-si 17086, KoreaR & BD Center, hy Co., Ltd., 22, Giheungdanji-ro 24beon-gil, Giheung-gu, Yongin-si 17086, KoreaR & BD Center, hy Co., Ltd., 22, Giheungdanji-ro 24beon-gil, Giheung-gu, Yongin-si 17086, KoreaR & BD Center, hy Co., Ltd., 22, Giheungdanji-ro 24beon-gil, Giheung-gu, Yongin-si 17086, KoreaR & BD Center, hy Co., Ltd., 22, Giheungdanji-ro 24beon-gil, Giheung-gu, Yongin-si 17086, KoreaVitamin B2, also known as riboflavin, is essential for maintaining human health. The purpose of this study was to isolate novel lactic acid bacteria that overproduce vitamin B2 and to validate their potential as probiotics. In this study, <i>Lactobacillus plantarum</i> HY7715 (HY7715) was selected among lactic acid bacteria isolated from Kimchi. HY7715 showed a very high riboflavin-producing ability compared to the control strain due to the high expression of <i>ribA</i>, <i>ribB</i>, <i>ribC</i>, <i>ribH</i>, and <i>ribG</i> genes. HY7715 produced 34.5 ± 2.41 mg/L of riboflavin for 24 h without consuming riboflavin in the medium under optimal growth conditions. It was able to produce riboflavin in an in vitro model of the intestinal environment. In addition, when riboflavin deficiency was induced in mice through nutritional restriction, higher levels of riboflavin were detected in plasma and urine in the HY7715 administration group than in the control group. HY7715 showed high survival rate in simulated gastrointestinal conditions and had antibiotic resistance below the cutoff MIC value suggested by the European Food Safety Authority; moreover, it did not cause hemolysis. In conclusion, HY7715 could be considered a beneficial probiotic strain for human and animal applications, suggesting that it could be a new alternative to address riboflavin deficiency.https://www.mdpi.com/2076-3417/11/13/5765riboflavinvitamin deficiencylactic acid bacteria<i>rib</i> operon |
| spellingShingle | Joo-Yun Kim Eun-Jung Choi Jae-Ho Lee Myeong-Seok Yoo Keon Heo Jae-Jung Shim Jung-Lyoul Lee Probiotic Potential of a Novel Vitamin B2-Overproducing <i>Lactobacillus plantarum</i> Strain, HY7715, Isolated from Kimchi Applied Sciences riboflavin vitamin deficiency lactic acid bacteria <i>rib</i> operon |
| title | Probiotic Potential of a Novel Vitamin B2-Overproducing <i>Lactobacillus plantarum</i> Strain, HY7715, Isolated from Kimchi |
| title_full | Probiotic Potential of a Novel Vitamin B2-Overproducing <i>Lactobacillus plantarum</i> Strain, HY7715, Isolated from Kimchi |
| title_fullStr | Probiotic Potential of a Novel Vitamin B2-Overproducing <i>Lactobacillus plantarum</i> Strain, HY7715, Isolated from Kimchi |
| title_full_unstemmed | Probiotic Potential of a Novel Vitamin B2-Overproducing <i>Lactobacillus plantarum</i> Strain, HY7715, Isolated from Kimchi |
| title_short | Probiotic Potential of a Novel Vitamin B2-Overproducing <i>Lactobacillus plantarum</i> Strain, HY7715, Isolated from Kimchi |
| title_sort | probiotic potential of a novel vitamin b2 overproducing i lactobacillus plantarum i strain hy7715 isolated from kimchi |
| topic | riboflavin vitamin deficiency lactic acid bacteria <i>rib</i> operon |
| url | https://www.mdpi.com/2076-3417/11/13/5765 |
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