Evaluation of Responsivity to Drought Stress Using Infrared Thermography and Chlorophyll Fluorescence in Potted Clones of <i>Cryptomeria japonica</i>
As climate change progresses, the breeding of drought-tolerant forest trees is necessary. Breeding drought-tolerant trees requires screening for drought stress using a large number of individuals and a high-throughput phenotyping method. The aim of this study was therefore to establish high-throughp...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/12/1/55 |
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| author | Yuya Takashima Yuichiro Hiraoka Michinari Matsushita Makoto Takahashi |
| author_facet | Yuya Takashima Yuichiro Hiraoka Michinari Matsushita Makoto Takahashi |
| author_sort | Yuya Takashima |
| collection | DOAJ |
| description | As climate change progresses, the breeding of drought-tolerant forest trees is necessary. Breeding drought-tolerant trees requires screening for drought stress using a large number of individuals and a high-throughput phenotyping method. The aim of this study was therefore to establish high-throughput methods for evaluating the clonal stress responses to drought stress using infrared thermography and chlorophyll fluorescence methods in <i>Cryptomeria japonica</i>. The stomatal conductance index (<i>Ig</i>), maximum photochemical quantum yield of photosystem II (<i>Fv</i>/<i>Fm</i>), and axial growth of four plus-tree clones of <i>C. japonica</i> planted in pots were measured weekly for 85 days after irrigation was stopped. The phenotypic trait responsivity to drought stress was estimated by a nonlinear mixed model and by introducing the cumulative water index, which considers the past history of the soil water environment. These methods and procedures enabled us to evaluate the clonal stress responses in <i>C. japonica</i> and could be applied to large-scale clone materials to promote the breeding program for drought tolerance. |
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| id | doaj.art-ce8dcba68bf949aebff46f5f312e3e31 |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-10T13:32:17Z |
| publishDate | 2021-01-01 |
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| series | Forests |
| spelling | doaj.art-ce8dcba68bf949aebff46f5f312e3e312023-11-21T07:50:27ZengMDPI AGForests1999-49072021-01-011215510.3390/f12010055Evaluation of Responsivity to Drought Stress Using Infrared Thermography and Chlorophyll Fluorescence in Potted Clones of <i>Cryptomeria japonica</i>Yuya Takashima0Yuichiro Hiraoka1Michinari Matsushita2Makoto Takahashi3Forest Tree Breeding Center, Forestry and Forest Products Research Institute, Forest Research and Management Organization, 3809-1 Ishi, Juo, Hitachi 319-1301, Ibaraki, JapanFaculty of Production and Environmental Management, Shizuoka Professional University of Agriculture, 678-1 Tomigaoka, Iwata 438-8577, Shizuoka, JapanForest Tree Breeding Center, Forestry and Forest Products Research Institute, Forest Research and Management Organization, 3809-1 Ishi, Juo, Hitachi 319-1301, Ibaraki, JapanForest Tree Breeding Center, Forestry and Forest Products Research Institute, Forest Research and Management Organization, 3809-1 Ishi, Juo, Hitachi 319-1301, Ibaraki, JapanAs climate change progresses, the breeding of drought-tolerant forest trees is necessary. Breeding drought-tolerant trees requires screening for drought stress using a large number of individuals and a high-throughput phenotyping method. The aim of this study was therefore to establish high-throughput methods for evaluating the clonal stress responses to drought stress using infrared thermography and chlorophyll fluorescence methods in <i>Cryptomeria japonica</i>. The stomatal conductance index (<i>Ig</i>), maximum photochemical quantum yield of photosystem II (<i>Fv</i>/<i>Fm</i>), and axial growth of four plus-tree clones of <i>C. japonica</i> planted in pots were measured weekly for 85 days after irrigation was stopped. The phenotypic trait responsivity to drought stress was estimated by a nonlinear mixed model and by introducing the cumulative water index, which considers the past history of the soil water environment. These methods and procedures enabled us to evaluate the clonal stress responses in <i>C. japonica</i> and could be applied to large-scale clone materials to promote the breeding program for drought tolerance.https://www.mdpi.com/1999-4907/12/1/55infrared thermographychlorophyll fluorescencecumulative drought stresshigh-throughput phenotyping<i>Cryptomeria japonica</i> |
| spellingShingle | Yuya Takashima Yuichiro Hiraoka Michinari Matsushita Makoto Takahashi Evaluation of Responsivity to Drought Stress Using Infrared Thermography and Chlorophyll Fluorescence in Potted Clones of <i>Cryptomeria japonica</i> Forests infrared thermography chlorophyll fluorescence cumulative drought stress high-throughput phenotyping <i>Cryptomeria japonica</i> |
| title | Evaluation of Responsivity to Drought Stress Using Infrared Thermography and Chlorophyll Fluorescence in Potted Clones of <i>Cryptomeria japonica</i> |
| title_full | Evaluation of Responsivity to Drought Stress Using Infrared Thermography and Chlorophyll Fluorescence in Potted Clones of <i>Cryptomeria japonica</i> |
| title_fullStr | Evaluation of Responsivity to Drought Stress Using Infrared Thermography and Chlorophyll Fluorescence in Potted Clones of <i>Cryptomeria japonica</i> |
| title_full_unstemmed | Evaluation of Responsivity to Drought Stress Using Infrared Thermography and Chlorophyll Fluorescence in Potted Clones of <i>Cryptomeria japonica</i> |
| title_short | Evaluation of Responsivity to Drought Stress Using Infrared Thermography and Chlorophyll Fluorescence in Potted Clones of <i>Cryptomeria japonica</i> |
| title_sort | evaluation of responsivity to drought stress using infrared thermography and chlorophyll fluorescence in potted clones of i cryptomeria japonica i |
| topic | infrared thermography chlorophyll fluorescence cumulative drought stress high-throughput phenotyping <i>Cryptomeria japonica</i> |
| url | https://www.mdpi.com/1999-4907/12/1/55 |
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