Anti-inflammatory effects of alkaloid enriched extract from roots of Eurycoma longifolia Jack
Objective: To examine the in vitro and in vivo anti-inflammatory effects of the alkaloid enriched extract (ELA) from the roots of Eurycoma longifolia. Methods: The in vitro antiinflammatory effects of ELA were evaluated by examining its inhibitory activities against nitric oxide (NO) production and...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2019-01-01
|
| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=1;spage=18;epage=23;aulast=Hien |
| _version_ | 1811207826213699584 |
|---|---|
| author | Dao Thi Thanh Hien Tran Phi Long Tran Phuong Thao Jeong-Hyung Lee Duong Thu Trang Nguyen Thi Thu Minh Pham Van Cuong Do Thi Ngoc Lan Nguyen Hai Dang Nguyen Tien Dat |
| author_facet | Dao Thi Thanh Hien Tran Phi Long Tran Phuong Thao Jeong-Hyung Lee Duong Thu Trang Nguyen Thi Thu Minh Pham Van Cuong Do Thi Ngoc Lan Nguyen Hai Dang Nguyen Tien Dat |
| author_sort | Dao Thi Thanh Hien |
| collection | DOAJ |
| description | Objective: To examine the in vitro and in vivo anti-inflammatory effects of the alkaloid enriched extract (ELA) from the roots of Eurycoma longifolia. Methods: The in vitro antiinflammatory effects of ELA were evaluated by examining its inhibitory activities against nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) expressions in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The level of NO produced in the culture media was determined by Griess method. The iNOS and COX-2 protein expressions were analyzed by Western blot. The in vivo effect of ELA was evaluated on LPS-induced septic shock in mice model. Mice mortality was monitored for 5 days after injection of LPS. The chemical contents of the ELA were determined by using various chromatographic and spectroscopic techniques. Results: The ELA was found to exhibit a significant anti-inflammatory effect in both in vitro and in vivo models. The results demonstrated that ELA dose-dependently inhibited LPS-induced NO production as well as the protein iNOS and COX-2 expressions. In the septic shock model, ELA dose-dependently protected mice from LPS-induced mortality. Further study on the isolated components of ELA indicated that 9,10-dimethoxycanthin-6-one may contribute significantly to the anti-inflammatory effects of the extract. Conclusions: These results suggest that ELA exhibits the anti-inflammatory activity via suppression of pro-inflammatory mediators such as NO, iNOS, and COX-2 and protects mice from LPS-induced mortality in septic shock model. |
| first_indexed | 2024-04-12T04:10:06Z |
| format | Article |
| id | doaj.art-ce8e54994d114458a217847206967a3e |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-04-12T04:10:06Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-ce8e54994d114458a217847206967a3e2022-12-22T03:48:31ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912019-01-0191182310.4103/2221-1691.250265Anti-inflammatory effects of alkaloid enriched extract from roots of Eurycoma longifolia JackDao Thi Thanh HienTran Phi LongTran Phuong ThaoJeong-Hyung LeeDuong Thu TrangNguyen Thi Thu MinhPham Van CuongDo Thi Ngoc LanNguyen Hai DangNguyen Tien DatObjective: To examine the in vitro and in vivo anti-inflammatory effects of the alkaloid enriched extract (ELA) from the roots of Eurycoma longifolia. Methods: The in vitro antiinflammatory effects of ELA were evaluated by examining its inhibitory activities against nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) expressions in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The level of NO produced in the culture media was determined by Griess method. The iNOS and COX-2 protein expressions were analyzed by Western blot. The in vivo effect of ELA was evaluated on LPS-induced septic shock in mice model. Mice mortality was monitored for 5 days after injection of LPS. The chemical contents of the ELA were determined by using various chromatographic and spectroscopic techniques. Results: The ELA was found to exhibit a significant anti-inflammatory effect in both in vitro and in vivo models. The results demonstrated that ELA dose-dependently inhibited LPS-induced NO production as well as the protein iNOS and COX-2 expressions. In the septic shock model, ELA dose-dependently protected mice from LPS-induced mortality. Further study on the isolated components of ELA indicated that 9,10-dimethoxycanthin-6-one may contribute significantly to the anti-inflammatory effects of the extract. Conclusions: These results suggest that ELA exhibits the anti-inflammatory activity via suppression of pro-inflammatory mediators such as NO, iNOS, and COX-2 and protects mice from LPS-induced mortality in septic shock model.http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=1;spage=18;epage=23;aulast=Hieneurycoma longifoliaanti-inflammationalkaloid910-dimethoxycanthin-6-one |
| spellingShingle | Dao Thi Thanh Hien Tran Phi Long Tran Phuong Thao Jeong-Hyung Lee Duong Thu Trang Nguyen Thi Thu Minh Pham Van Cuong Do Thi Ngoc Lan Nguyen Hai Dang Nguyen Tien Dat Anti-inflammatory effects of alkaloid enriched extract from roots of Eurycoma longifolia Jack Asian Pacific Journal of Tropical Biomedicine eurycoma longifolia anti-inflammation alkaloid 9 10-dimethoxycanthin-6-one |
| title | Anti-inflammatory effects of alkaloid enriched extract from roots of Eurycoma longifolia Jack |
| title_full | Anti-inflammatory effects of alkaloid enriched extract from roots of Eurycoma longifolia Jack |
| title_fullStr | Anti-inflammatory effects of alkaloid enriched extract from roots of Eurycoma longifolia Jack |
| title_full_unstemmed | Anti-inflammatory effects of alkaloid enriched extract from roots of Eurycoma longifolia Jack |
| title_short | Anti-inflammatory effects of alkaloid enriched extract from roots of Eurycoma longifolia Jack |
| title_sort | anti inflammatory effects of alkaloid enriched extract from roots of eurycoma longifolia jack |
| topic | eurycoma longifolia anti-inflammation alkaloid 9 10-dimethoxycanthin-6-one |
| url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=1;spage=18;epage=23;aulast=Hien |
| work_keys_str_mv | AT daothithanhhien antiinflammatoryeffectsofalkaloidenrichedextractfromrootsofeurycomalongifoliajack AT tranphilong antiinflammatoryeffectsofalkaloidenrichedextractfromrootsofeurycomalongifoliajack AT tranphuongthao antiinflammatoryeffectsofalkaloidenrichedextractfromrootsofeurycomalongifoliajack AT jeonghyunglee antiinflammatoryeffectsofalkaloidenrichedextractfromrootsofeurycomalongifoliajack AT duongthutrang antiinflammatoryeffectsofalkaloidenrichedextractfromrootsofeurycomalongifoliajack AT nguyenthithuminh antiinflammatoryeffectsofalkaloidenrichedextractfromrootsofeurycomalongifoliajack AT phamvancuong antiinflammatoryeffectsofalkaloidenrichedextractfromrootsofeurycomalongifoliajack AT dothingoclan antiinflammatoryeffectsofalkaloidenrichedextractfromrootsofeurycomalongifoliajack AT nguyenhaidang antiinflammatoryeffectsofalkaloidenrichedextractfromrootsofeurycomalongifoliajack AT nguyentiendat antiinflammatoryeffectsofalkaloidenrichedextractfromrootsofeurycomalongifoliajack |