Effect of hot-melt extruded Morus alba leaves on intestinal microflora and epithelial cells
Although rutin and isoquercitrin have many effects, they are insoluble substances, making it difficult to obtain pure substances. This study was to investigate whether Morus alba leaves containing rutin and isoquercitrin could improve intestinal health by making a sustained-release formulation throu...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023111625 |
| _version_ | 1797336919533682688 |
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| author | Hyun Bok Kim Eun Ji Go Jong-Suep Baek |
| author_facet | Hyun Bok Kim Eun Ji Go Jong-Suep Baek |
| author_sort | Hyun Bok Kim |
| collection | DOAJ |
| description | Although rutin and isoquercitrin have many effects, they are insoluble substances, making it difficult to obtain pure substances. This study was to investigate whether Morus alba leaves containing rutin and isoquercitrin could improve intestinal health by making a sustained-release formulation through a hot-melt extrusion (HME) process with improved stability and solubility and determine whether it could upregulate the balance of intestinal microorganisms and intestinal epithelial cells. A sustained-release formulation was prepared by the HME process using Morus alba leaves and a hydrophilic polymer matrix. Antibacterial activities of pathogenic microorganisms (Escherichia coli, Streptococcus aureus, Enterococcus faecalis) and proliferative effect of probiotics (Lactobacillus rhamnosus, Pediococcus pentosaceus) were tested against intestinal microorganisms. Regarding intestinal epithelial cells, a co-culture model of Caco-2 cells and RAW 264.7 cells was used. It was confirmed that the extrudate exhibited high antibacterial activities against pathogenic microorganisms and affected the proliferation of probiotics. Furthermore, after inducing inflammation through LPS, it recovered transepithelial electrical resistance-increased levels of tight junction proteins and decreased expression levels of pro-inflammatory cytokines. HME of Morus alba leaves containing rutin and isoquercitrin can upregulate intestinal microbial balance and intestinal epithelial cells. |
| first_indexed | 2024-03-08T09:01:53Z |
| format | Article |
| id | doaj.art-ce8ee64c7ff649e1a56b2d6bfcdf8c5b |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T09:01:53Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-ce8ee64c7ff649e1a56b2d6bfcdf8c5b2024-02-01T06:34:16ZengElsevierHeliyon2405-84402024-01-01101e23954Effect of hot-melt extruded Morus alba leaves on intestinal microflora and epithelial cellsHyun Bok Kim0Eun Ji Go1Jong-Suep Baek2National Institute of Agricultural Sciences, RDA, Wanju 55365, South KoreaDepartment of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, South KoreaDepartment of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, South Korea; Department of Bio-Functional Materials, Kangwon National University, Samcheok 25949. South Korea; BeNatureBioLab, Chuncheon 24206, South Korea; Corresponding author. Department of Bio-Functional Materials, Kangwon National University, Samcheok 25949. South Korea.Although rutin and isoquercitrin have many effects, they are insoluble substances, making it difficult to obtain pure substances. This study was to investigate whether Morus alba leaves containing rutin and isoquercitrin could improve intestinal health by making a sustained-release formulation through a hot-melt extrusion (HME) process with improved stability and solubility and determine whether it could upregulate the balance of intestinal microorganisms and intestinal epithelial cells. A sustained-release formulation was prepared by the HME process using Morus alba leaves and a hydrophilic polymer matrix. Antibacterial activities of pathogenic microorganisms (Escherichia coli, Streptococcus aureus, Enterococcus faecalis) and proliferative effect of probiotics (Lactobacillus rhamnosus, Pediococcus pentosaceus) were tested against intestinal microorganisms. Regarding intestinal epithelial cells, a co-culture model of Caco-2 cells and RAW 264.7 cells was used. It was confirmed that the extrudate exhibited high antibacterial activities against pathogenic microorganisms and affected the proliferation of probiotics. Furthermore, after inducing inflammation through LPS, it recovered transepithelial electrical resistance-increased levels of tight junction proteins and decreased expression levels of pro-inflammatory cytokines. HME of Morus alba leaves containing rutin and isoquercitrin can upregulate intestinal microbial balance and intestinal epithelial cells.http://www.sciencedirect.com/science/article/pii/S2405844023111625Morus alba leavesHot-melt extrusionProbioticsGut microbiotaTight junctionEncapsulation |
| spellingShingle | Hyun Bok Kim Eun Ji Go Jong-Suep Baek Effect of hot-melt extruded Morus alba leaves on intestinal microflora and epithelial cells Heliyon Morus alba leaves Hot-melt extrusion Probiotics Gut microbiota Tight junction Encapsulation |
| title | Effect of hot-melt extruded Morus alba leaves on intestinal microflora and epithelial cells |
| title_full | Effect of hot-melt extruded Morus alba leaves on intestinal microflora and epithelial cells |
| title_fullStr | Effect of hot-melt extruded Morus alba leaves on intestinal microflora and epithelial cells |
| title_full_unstemmed | Effect of hot-melt extruded Morus alba leaves on intestinal microflora and epithelial cells |
| title_short | Effect of hot-melt extruded Morus alba leaves on intestinal microflora and epithelial cells |
| title_sort | effect of hot melt extruded morus alba leaves on intestinal microflora and epithelial cells |
| topic | Morus alba leaves Hot-melt extrusion Probiotics Gut microbiota Tight junction Encapsulation |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023111625 |
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