Anti-inflammatory effect of Rhododendron brachycarpum D. Don ex G. Don leaves extract on dermatitis
Introduction: Rhododendron brachycarpum D. Don ex G. Don(RbGD) leaves have traditionally been used to treat diabetes, rheumatoid arthritis, hypertension, and skin ailments. Although RbGD leaves are used as a medicinal plant, there is no scientific evidence to support skin treatment. Therefore, the p...
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| Format: | Article |
| Language: | English |
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Shahrekord University of Medical Sciences
2023-01-01
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| Series: | Journal of HerbMed Pharmacology |
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| Online Access: | http://herbmedpharmacol.com/PDF/jhp-12-73.pdf |
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| author | Jinjoo Kang YoungJae Kim Jae-Min Chung Namkyung Kim Young-Ae Choi Eun-Nam Kim Gil-Saeng Jeong Sang-Yong Kim Sang-Hyun Kim |
| author_facet | Jinjoo Kang YoungJae Kim Jae-Min Chung Namkyung Kim Young-Ae Choi Eun-Nam Kim Gil-Saeng Jeong Sang-Yong Kim Sang-Hyun Kim |
| author_sort | Jinjoo Kang |
| collection | DOAJ |
| description | Introduction: Rhododendron brachycarpum D. Don ex G. Don(RbGD) leaves have traditionally been used to treat diabetes, rheumatoid arthritis, hypertension, and skin ailments. Although RbGD leaves are used as a medicinal plant, there is no scientific evidence to support skin treatment. Therefore, the purpose of this study was to look into the anti-inflammatory effect of an ethanolic extract of RbGD on skin disease, specifically atopic dermatitis (AD). Methods: The anti-inflammatory effect of RbGD ethanol leaf extract (RbGDE) on tumor necrosis factor-α (TNF-α)/interferon-γ (IFN-γ)-activated keratinocytes was evaluated using MTT, qPCR, ELISA, and Western blot Procedures. The therapeutic effects of RbGDE were evaluated in vivo inflammatory responses by histological observation, quantitative polymerase chain reaction (qPCR), and ELISA using the 1-chloro-2,4-dinitrobenzene (DNCB)/Dermatophagoides farina extract (DfE)-induced AD-like skin mouse model. Results: RbGDE showed the protective effect against irritating and stimulating substances (H2 O2 and TNF-α/IFN-γ) and inhibited TNF-α/IFN-γ-activated keratinocytes by inhibiting the p38 mitogen-activated protein kinase and nuclear factor-kappa B activation. Furthermore, topical RbGDE treatment reduced the AD features such as thickened skin, erythema, immune cells infiltration (eosinophils and mast cells), and AD-related cytokines (IL-12a, IL-1β, IL-4, and TSLP) in the ear tissues of DNCB/DfE-induced mice. The RbGDE also reduced histamine and immunoglobulins (Igs) levels in the serum, including DfE-specific IgE, total IgE, and IgG2a. Conclusion: RbGD leaf extract had an anti-inflammatory effect on dermatitis by reducing inflammatory mediators, indicating that it might be used to treat skin disease. |
| first_indexed | 2024-04-09T16:01:09Z |
| format | Article |
| id | doaj.art-ce905143e1d0464eaf7658980cea73b0 |
| institution | Directory Open Access Journal |
| issn | 2345-5004 |
| language | English |
| last_indexed | 2024-04-09T16:01:09Z |
| publishDate | 2023-01-01 |
| publisher | Shahrekord University of Medical Sciences |
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| series | Journal of HerbMed Pharmacology |
| spelling | doaj.art-ce905143e1d0464eaf7658980cea73b02023-04-25T12:41:54ZengShahrekord University of Medical SciencesJournal of HerbMed Pharmacology2345-50042023-01-01121738310.34172/jhp.2023.06jhp-42373Anti-inflammatory effect of Rhododendron brachycarpum D. Don ex G. Don leaves extract on dermatitisJinjoo Kang0YoungJae Kim1Jae-Min Chung2Namkyung Kim3Young-Ae Choi4Eun-Nam Kim5Gil-Saeng Jeong6Sang-Yong Kim7Sang-Hyun Kim8Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 41944, South KoreaDepartment of Gardens Education, Korea National Arboretum, Pocheon 11186, South KoreaDivision of Plant Resources, Korea National Arboretum, Yangpyeong 12519, South KoreaDepartment of Pharmacology, School of Medicine, Kyungpook National University, Daegu 41944, South KoreaDepartment of Pharmacology, School of Medicine, Kyungpook National University, Daegu 41944, South KoreaCollege of Pharmacy, Chungnam National University, Daejeon 34134, South KoreaCollege of Pharmacy, Chungnam National University, Daejeon 34134, South KoreaDivision of Plant Resources, Korea National Arboretum, Yangpyeong 12519, South KoreaDepartment of Pharmacology, School of Medicine, Kyungpook National University, Daegu 41944, South KoreaIntroduction: Rhododendron brachycarpum D. Don ex G. Don(RbGD) leaves have traditionally been used to treat diabetes, rheumatoid arthritis, hypertension, and skin ailments. Although RbGD leaves are used as a medicinal plant, there is no scientific evidence to support skin treatment. Therefore, the purpose of this study was to look into the anti-inflammatory effect of an ethanolic extract of RbGD on skin disease, specifically atopic dermatitis (AD). Methods: The anti-inflammatory effect of RbGD ethanol leaf extract (RbGDE) on tumor necrosis factor-α (TNF-α)/interferon-γ (IFN-γ)-activated keratinocytes was evaluated using MTT, qPCR, ELISA, and Western blot Procedures. The therapeutic effects of RbGDE were evaluated in vivo inflammatory responses by histological observation, quantitative polymerase chain reaction (qPCR), and ELISA using the 1-chloro-2,4-dinitrobenzene (DNCB)/Dermatophagoides farina extract (DfE)-induced AD-like skin mouse model. Results: RbGDE showed the protective effect against irritating and stimulating substances (H2 O2 and TNF-α/IFN-γ) and inhibited TNF-α/IFN-γ-activated keratinocytes by inhibiting the p38 mitogen-activated protein kinase and nuclear factor-kappa B activation. Furthermore, topical RbGDE treatment reduced the AD features such as thickened skin, erythema, immune cells infiltration (eosinophils and mast cells), and AD-related cytokines (IL-12a, IL-1β, IL-4, and TSLP) in the ear tissues of DNCB/DfE-induced mice. The RbGDE also reduced histamine and immunoglobulins (Igs) levels in the serum, including DfE-specific IgE, total IgE, and IgG2a. Conclusion: RbGD leaf extract had an anti-inflammatory effect on dermatitis by reducing inflammatory mediators, indicating that it might be used to treat skin disease.http://herbmedpharmacol.com/PDF/jhp-12-73.pdfatopic dermatitisherbal medicinekeratinocytestherapeuticstopical administration |
| spellingShingle | Jinjoo Kang YoungJae Kim Jae-Min Chung Namkyung Kim Young-Ae Choi Eun-Nam Kim Gil-Saeng Jeong Sang-Yong Kim Sang-Hyun Kim Anti-inflammatory effect of Rhododendron brachycarpum D. Don ex G. Don leaves extract on dermatitis Journal of HerbMed Pharmacology atopic dermatitis herbal medicine keratinocytes therapeutics topical administration |
| title | Anti-inflammatory effect of Rhododendron brachycarpum D. Don ex G. Don leaves extract on dermatitis |
| title_full | Anti-inflammatory effect of Rhododendron brachycarpum D. Don ex G. Don leaves extract on dermatitis |
| title_fullStr | Anti-inflammatory effect of Rhododendron brachycarpum D. Don ex G. Don leaves extract on dermatitis |
| title_full_unstemmed | Anti-inflammatory effect of Rhododendron brachycarpum D. Don ex G. Don leaves extract on dermatitis |
| title_short | Anti-inflammatory effect of Rhododendron brachycarpum D. Don ex G. Don leaves extract on dermatitis |
| title_sort | anti inflammatory effect of rhododendron brachycarpum d don ex g don leaves extract on dermatitis |
| topic | atopic dermatitis herbal medicine keratinocytes therapeutics topical administration |
| url | http://herbmedpharmacol.com/PDF/jhp-12-73.pdf |
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