Tetrachloromethane-Degrading Bacterial Enrichment Cultures and Isolates from a Contaminated Aquifer
The prokaryotic community of a groundwater aquifer exposed to high concentrations of tetrachloromethane (CCl4) for more than three decades was followed by terminal restriction fragment length polymorphism (T-RFLP) during pump-and-treat remediation at the contamination source. Bacterial enrichments a...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2015-07-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2076-2607/3/3/327 |
| _version_ | 1818136715784617984 |
|---|---|
| author | Christian Penny Christelle Gruffaz Thierry Nadalig Henry-Michel Cauchie Stéphane Vuilleumier Françoise Bringel |
| author_facet | Christian Penny Christelle Gruffaz Thierry Nadalig Henry-Michel Cauchie Stéphane Vuilleumier Françoise Bringel |
| author_sort | Christian Penny |
| collection | DOAJ |
| description | The prokaryotic community of a groundwater aquifer exposed to high concentrations of tetrachloromethane (CCl4) for more than three decades was followed by terminal restriction fragment length polymorphism (T-RFLP) during pump-and-treat remediation at the contamination source. Bacterial enrichments and isolates were obtained under selective anoxic conditions, and degraded 10 mg·L−1 CCl4, with less than 10% transient formation of chloroform. Dichloromethane and chloromethane were not detected. Several tetrachloromethane-degrading strains were isolated from these enrichments, including bacteria from the Klebsiella and Clostridium genera closely related to previously described CCl4 degrading bacteria, and strain TM1, assigned to the genus Pelosinus, for which this property was not yet described. Pelosinus sp. TM1, an oxygen-tolerant, Gram-positive bacterium with strictly anaerobic metabolism, excreted a thermostable metabolite into the culture medium that allowed extracellular CCl4 transformation. As estimated by T-RFLP, phylotypes of CCl4-degrading enrichment cultures represented less than 7%, and archaeal and Pelosinus strains less than 0.5% of the total prokaryotic groundwater community. |
| first_indexed | 2024-12-11T09:44:49Z |
| format | Article |
| id | doaj.art-ce90ce4b02d040e3a2be7745a532bc4e |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-11T09:44:49Z |
| publishDate | 2015-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-ce90ce4b02d040e3a2be7745a532bc4e2022-12-22T01:12:35ZengMDPI AGMicroorganisms2076-26072015-07-013332734310.3390/microorganisms3030327microorganisms3030327Tetrachloromethane-Degrading Bacterial Enrichment Cultures and Isolates from a Contaminated AquiferChristian Penny0Christelle Gruffaz1Thierry Nadalig2Henry-Michel Cauchie3Stéphane Vuilleumier4Françoise Bringel5CNRS Génétique Moléculaire, Génomique, Microbiologie, Université de Strasbourg, UMR 7156 UNISTRA-CNRS, 28 rue Goethe, 67083 Strasbourg, FranceCNRS Génétique Moléculaire, Génomique, Microbiologie, Université de Strasbourg, UMR 7156 UNISTRA-CNRS, 28 rue Goethe, 67083 Strasbourg, FranceCNRS Génétique Moléculaire, Génomique, Microbiologie, Université de Strasbourg, UMR 7156 UNISTRA-CNRS, 28 rue Goethe, 67083 Strasbourg, FranceEnvironmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), 5 avenue des Hauts-Fourneaux, 4362 Esch/Alzette, LuxembourgCNRS Génétique Moléculaire, Génomique, Microbiologie, Université de Strasbourg, UMR 7156 UNISTRA-CNRS, 28 rue Goethe, 67083 Strasbourg, FranceCNRS Génétique Moléculaire, Génomique, Microbiologie, Université de Strasbourg, UMR 7156 UNISTRA-CNRS, 28 rue Goethe, 67083 Strasbourg, FranceThe prokaryotic community of a groundwater aquifer exposed to high concentrations of tetrachloromethane (CCl4) for more than three decades was followed by terminal restriction fragment length polymorphism (T-RFLP) during pump-and-treat remediation at the contamination source. Bacterial enrichments and isolates were obtained under selective anoxic conditions, and degraded 10 mg·L−1 CCl4, with less than 10% transient formation of chloroform. Dichloromethane and chloromethane were not detected. Several tetrachloromethane-degrading strains were isolated from these enrichments, including bacteria from the Klebsiella and Clostridium genera closely related to previously described CCl4 degrading bacteria, and strain TM1, assigned to the genus Pelosinus, for which this property was not yet described. Pelosinus sp. TM1, an oxygen-tolerant, Gram-positive bacterium with strictly anaerobic metabolism, excreted a thermostable metabolite into the culture medium that allowed extracellular CCl4 transformation. As estimated by T-RFLP, phylotypes of CCl4-degrading enrichment cultures represented less than 7%, and archaeal and Pelosinus strains less than 0.5% of the total prokaryotic groundwater community.http://www.mdpi.com/2076-2607/3/3/327C1 compound biodegradationtetrachloromethanecarbon tetrachloridebacterial communitiesbacterial enrichment culturesco-metabolismchlorinated solventsPelosinus sp. TM1 |
| spellingShingle | Christian Penny Christelle Gruffaz Thierry Nadalig Henry-Michel Cauchie Stéphane Vuilleumier Françoise Bringel Tetrachloromethane-Degrading Bacterial Enrichment Cultures and Isolates from a Contaminated Aquifer Microorganisms C1 compound biodegradation tetrachloromethane carbon tetrachloride bacterial communities bacterial enrichment cultures co-metabolism chlorinated solvents Pelosinus sp. TM1 |
| title | Tetrachloromethane-Degrading Bacterial Enrichment Cultures and Isolates from a Contaminated Aquifer |
| title_full | Tetrachloromethane-Degrading Bacterial Enrichment Cultures and Isolates from a Contaminated Aquifer |
| title_fullStr | Tetrachloromethane-Degrading Bacterial Enrichment Cultures and Isolates from a Contaminated Aquifer |
| title_full_unstemmed | Tetrachloromethane-Degrading Bacterial Enrichment Cultures and Isolates from a Contaminated Aquifer |
| title_short | Tetrachloromethane-Degrading Bacterial Enrichment Cultures and Isolates from a Contaminated Aquifer |
| title_sort | tetrachloromethane degrading bacterial enrichment cultures and isolates from a contaminated aquifer |
| topic | C1 compound biodegradation tetrachloromethane carbon tetrachloride bacterial communities bacterial enrichment cultures co-metabolism chlorinated solvents Pelosinus sp. TM1 |
| url | http://www.mdpi.com/2076-2607/3/3/327 |
| work_keys_str_mv | AT christianpenny tetrachloromethanedegradingbacterialenrichmentculturesandisolatesfromacontaminatedaquifer AT christellegruffaz tetrachloromethanedegradingbacterialenrichmentculturesandisolatesfromacontaminatedaquifer AT thierrynadalig tetrachloromethanedegradingbacterialenrichmentculturesandisolatesfromacontaminatedaquifer AT henrymichelcauchie tetrachloromethanedegradingbacterialenrichmentculturesandisolatesfromacontaminatedaquifer AT stephanevuilleumier tetrachloromethanedegradingbacterialenrichmentculturesandisolatesfromacontaminatedaquifer AT francoisebringel tetrachloromethanedegradingbacterialenrichmentculturesandisolatesfromacontaminatedaquifer |