Analysis of Liver Transcriptome in Broilers with Ascites and Regulation by L-Carnitine
Two experiments were conducted using male Ross-308 broilers. Exp. 1 was to determine the differences in liver transcriptomic profile of healthy broilers at 39 days (D39) vs. 21 days of age (D21), and of ascites vs. healthy broilers at D39 by GeneChips, under a low-temperature environment. Differen...
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2013-04-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/50/2/50_0120124/_pdf/-char/en |
| _version_ | 1797816210331533312 |
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| author | Yongwei Wang Yuming Guo Dong Ning Yunzhi Peng Ying Yang Dan Liu |
| author_facet | Yongwei Wang Yuming Guo Dong Ning Yunzhi Peng Ying Yang Dan Liu |
| author_sort | Yongwei Wang |
| collection | DOAJ |
| description | Two experiments were conducted using male Ross-308 broilers. Exp. 1 was to determine the differences in liver transcriptomic profile of healthy broilers at 39 days (D39) vs. 21 days of age (D21), and of ascites vs. healthy broilers at D39 by GeneChips, under a low-temperature environment. Differentially expressed genes related to liver antioxidative capacity and energy metabolism were screened. Exp. 2 was to analyze the regulatory roles of L-carnitine on genes and biochemical parameters involved in liver oxidoreduction and energy metabolism. The results showed that the expression of 790 genes in D39 vs D21 healthy broilers (386 up- and 404 down-regulated) and expression of 178 genes in ascites vs. healthy broilers on D39 (128 up- and 50 down-regulated) was changed. The analysis of pathway and GO indicated that genes related to amino acid metabolism, fatty acid metabolism, Krebs cycle, carbohydrate metabolism, vitamin metabolism, cell apoptosis, immune response, oxidoreduction, angiogenesis, nitric oxide formation and erythrocyte differentiation were involved in the development of ascites. Liver MDA level of ascites broilers was significantly increased, but the activity of T-SOD was significantly decreased than healthy broilers. The activity of liver HK, MDH, PK and Na+-K+-ATPase was significantly lower in ascites broilers. The expression of liver HKDC1 and PCK1 was significantly down-regulated, and that of HNF-4α and AKR1B10 expression was significantly up-regulated. L-carnitine supplementation significantly increased the activity of T-SOD and PK, and significantly up-regulated HKDC1 and HNF-4α expression. It can be concluded that genes involved in many biological processes are differentially expressed due to ascites. Liver oxidation damage and energy generation obstruction were found in broilers with ascites. L-carnitine can alleviate the liver oxidative damage and promote the generation and utilization of energy. The present results demonstrated that L-carnitine could serve as a potential regulatory agent to reduce ascites susceptibility and mortality. |
| first_indexed | 2024-03-13T08:34:19Z |
| format | Article |
| id | doaj.art-ce99d52e8f234a1eb714ffc9031fd717 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:34:19Z |
| publishDate | 2013-04-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-ce99d52e8f234a1eb714ffc9031fd7172023-05-31T01:23:33ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862013-04-0150212613710.2141/jpsa.0120124jpsaAnalysis of Liver Transcriptome in Broilers with Ascites and Regulation by L-CarnitineYongwei Wang0Yuming Guo1Dong Ning2Yunzhi Peng3Ying Yang4Dan Liu5State Key Lab of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, ChinaState Key Lab of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, ChinaState Key Lab of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, ChinaState Key Lab of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, ChinaState Key Lab of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, ChinaState Key Lab of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, ChinaTwo experiments were conducted using male Ross-308 broilers. Exp. 1 was to determine the differences in liver transcriptomic profile of healthy broilers at 39 days (D39) vs. 21 days of age (D21), and of ascites vs. healthy broilers at D39 by GeneChips, under a low-temperature environment. Differentially expressed genes related to liver antioxidative capacity and energy metabolism were screened. Exp. 2 was to analyze the regulatory roles of L-carnitine on genes and biochemical parameters involved in liver oxidoreduction and energy metabolism. The results showed that the expression of 790 genes in D39 vs D21 healthy broilers (386 up- and 404 down-regulated) and expression of 178 genes in ascites vs. healthy broilers on D39 (128 up- and 50 down-regulated) was changed. The analysis of pathway and GO indicated that genes related to amino acid metabolism, fatty acid metabolism, Krebs cycle, carbohydrate metabolism, vitamin metabolism, cell apoptosis, immune response, oxidoreduction, angiogenesis, nitric oxide formation and erythrocyte differentiation were involved in the development of ascites. Liver MDA level of ascites broilers was significantly increased, but the activity of T-SOD was significantly decreased than healthy broilers. The activity of liver HK, MDH, PK and Na+-K+-ATPase was significantly lower in ascites broilers. The expression of liver HKDC1 and PCK1 was significantly down-regulated, and that of HNF-4α and AKR1B10 expression was significantly up-regulated. L-carnitine supplementation significantly increased the activity of T-SOD and PK, and significantly up-regulated HKDC1 and HNF-4α expression. It can be concluded that genes involved in many biological processes are differentially expressed due to ascites. Liver oxidation damage and energy generation obstruction were found in broilers with ascites. L-carnitine can alleviate the liver oxidative damage and promote the generation and utilization of energy. The present results demonstrated that L-carnitine could serve as a potential regulatory agent to reduce ascites susceptibility and mortality.https://www.jstage.jst.go.jp/article/jpsa/50/2/50_0120124/_pdf/-char/enbroiler ascitesl-carnitinenutritional regulationtranscriptome |
| spellingShingle | Yongwei Wang Yuming Guo Dong Ning Yunzhi Peng Ying Yang Dan Liu Analysis of Liver Transcriptome in Broilers with Ascites and Regulation by L-Carnitine The Journal of Poultry Science broiler ascites l-carnitine nutritional regulation transcriptome |
| title | Analysis of Liver Transcriptome in Broilers with Ascites and Regulation by L-Carnitine |
| title_full | Analysis of Liver Transcriptome in Broilers with Ascites and Regulation by L-Carnitine |
| title_fullStr | Analysis of Liver Transcriptome in Broilers with Ascites and Regulation by L-Carnitine |
| title_full_unstemmed | Analysis of Liver Transcriptome in Broilers with Ascites and Regulation by L-Carnitine |
| title_short | Analysis of Liver Transcriptome in Broilers with Ascites and Regulation by L-Carnitine |
| title_sort | analysis of liver transcriptome in broilers with ascites and regulation by l carnitine |
| topic | broiler ascites l-carnitine nutritional regulation transcriptome |
| url | https://www.jstage.jst.go.jp/article/jpsa/50/2/50_0120124/_pdf/-char/en |
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