Bioactivities of endophytic Penicillia from Cupressaceae
The cypress family, Cupressaceae, has a global dispersion. Currently, endophytic microorganisms from plants are being investigated for their diversity and bioactivities. Here, we aimed at exploration and characterization of cultivable endophytic fungi from foliar tissues of Cupressaceae, i.e. Cupres...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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University of Tarbiat Modares
2013-12-01
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| Series: | Journal of Crop Protection |
| Subjects: | |
| Online Access: | http://jcp.modares.ac.ir/article-3-11659-en.html |
| _version_ | 1828731279496970240 |
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| author | Mahdieh Hosseini Moghaddam Jalal Soltani Freydoun Babolhavaeji Javad Hamzei Sonbol Nazeri Soheila Mirzaei |
| author_facet | Mahdieh Hosseini Moghaddam Jalal Soltani Freydoun Babolhavaeji Javad Hamzei Sonbol Nazeri Soheila Mirzaei |
| author_sort | Mahdieh Hosseini Moghaddam |
| collection | DOAJ |
| description | The cypress family, Cupressaceae, has a global dispersion. Currently, endophytic microorganisms from plants are being investigated for their diversity and bioactivities. Here, we aimed at exploration and characterization of cultivable endophytic fungi from foliar tissues of Cupressaceae, i.e. Cupressus arizonica, C. sempervirens var. cereiformis, C. sempervirens var. fastigiata, Juniperus excelsa, Juniperus sp. and Thuja orientalis. Asymptomatic fresh foliar tissues, collected from mature healthy plants, were sterilized and the inner layers were plated on culture media at 26-28 °C for 2-12 weeks, until fungal colonies emerged and were purified. Endophytic Penicillia i.e. Penicillium aurantiogriseum, P. chrysogenum, P. commune, P. echinulatum, P. expansum and P. viridicatum were the dominant fungi recovered. Results indicated that both host plant and geographical location of sampling affected the biodiversity and bioactivity of endophytic Penicillia. Results also indicated that those endophytic Penicillia had significant bioactivities. According to our results, both intra-and extra-cellular secondary metabolites from all isolated Penicillia had significant cytotoxic and antifungal effects against the model fungus Pyricularia oryzae and cypress fungal phytopathogens Diplodia seriata, Phaeobotryon cupressi and Spencermartinsia viticola. Further studies indicated the significant antimicrobial bioactivities of superior Penicillia against model bacteria. Altogether, this study highlights, for the first time, the biodiversity of endophytic Penicillia from Cupressaceae plants and documents their significance for agrochemical/drug discovery and for plant disease biocontrol. |
| first_indexed | 2024-04-12T17:43:45Z |
| format | Article |
| id | doaj.art-ce9dab223cac48c9bb988242a42e4721 |
| institution | Directory Open Access Journal |
| issn | 2251-9041 2251-905X |
| language | English |
| last_indexed | 2024-04-12T17:43:45Z |
| publishDate | 2013-12-01 |
| publisher | University of Tarbiat Modares |
| record_format | Article |
| series | Journal of Crop Protection |
| spelling | doaj.art-ce9dab223cac48c9bb988242a42e47212022-12-22T03:22:44ZengUniversity of Tarbiat ModaresJournal of Crop Protection2251-90412251-905X2013-12-0124421433Bioactivities of endophytic Penicillia from CupressaceaeMahdieh Hosseini Moghaddam0Jalal Soltani1Freydoun Babolhavaeji2Javad Hamzei3Sonbol Nazeri4Soheila Mirzaei5 Phytopathology Department, Bu-Ali Sina University, Hamedan, Iran. Phytopathology Department, Bu-Ali Sina University, Hamedan, Iran. Phytopathology Department, Bu-Ali Sina University, Hamedan, Iran. Agronomy and Plant Breeding Department, Bu-Ali Sina University, Hamedan, Iran. Biotechnology Department, Bu-Ali Sina University, Hamedan, Iran. Phytopathology Department, Bu-Ali Sina University, Hamedan, Iran. The cypress family, Cupressaceae, has a global dispersion. Currently, endophytic microorganisms from plants are being investigated for their diversity and bioactivities. Here, we aimed at exploration and characterization of cultivable endophytic fungi from foliar tissues of Cupressaceae, i.e. Cupressus arizonica, C. sempervirens var. cereiformis, C. sempervirens var. fastigiata, Juniperus excelsa, Juniperus sp. and Thuja orientalis. Asymptomatic fresh foliar tissues, collected from mature healthy plants, were sterilized and the inner layers were plated on culture media at 26-28 °C for 2-12 weeks, until fungal colonies emerged and were purified. Endophytic Penicillia i.e. Penicillium aurantiogriseum, P. chrysogenum, P. commune, P. echinulatum, P. expansum and P. viridicatum were the dominant fungi recovered. Results indicated that both host plant and geographical location of sampling affected the biodiversity and bioactivity of endophytic Penicillia. Results also indicated that those endophytic Penicillia had significant bioactivities. According to our results, both intra-and extra-cellular secondary metabolites from all isolated Penicillia had significant cytotoxic and antifungal effects against the model fungus Pyricularia oryzae and cypress fungal phytopathogens Diplodia seriata, Phaeobotryon cupressi and Spencermartinsia viticola. Further studies indicated the significant antimicrobial bioactivities of superior Penicillia against model bacteria. Altogether, this study highlights, for the first time, the biodiversity of endophytic Penicillia from Cupressaceae plants and documents their significance for agrochemical/drug discovery and for plant disease biocontrol.http://jcp.modares.ac.ir/article-3-11659-en.htmlpenicilliumendophytecupressaceaeantiproliferativeantifungalantimicrobialbiocontrol |
| spellingShingle | Mahdieh Hosseini Moghaddam Jalal Soltani Freydoun Babolhavaeji Javad Hamzei Sonbol Nazeri Soheila Mirzaei Bioactivities of endophytic Penicillia from Cupressaceae Journal of Crop Protection penicillium endophyte cupressaceae antiproliferative antifungal antimicrobial biocontrol |
| title | Bioactivities of endophytic Penicillia from Cupressaceae |
| title_full | Bioactivities of endophytic Penicillia from Cupressaceae |
| title_fullStr | Bioactivities of endophytic Penicillia from Cupressaceae |
| title_full_unstemmed | Bioactivities of endophytic Penicillia from Cupressaceae |
| title_short | Bioactivities of endophytic Penicillia from Cupressaceae |
| title_sort | bioactivities of endophytic penicillia from cupressaceae |
| topic | penicillium endophyte cupressaceae antiproliferative antifungal antimicrobial biocontrol |
| url | http://jcp.modares.ac.ir/article-3-11659-en.html |
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