Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells
The purpose of this work is to study the influence of extracellular ATP (adenosine-3-phosphate), which is an activator of purinergic receptors, on contractive activity of rat aortic ring segments precontracted by α1-adrenoreceptors activation with phenylephrine and evaluate the impact of potassium c...
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| Format: | Article |
| Language: | English |
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Siberian State Medical University (Tomsk)
2017-01-01
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| Series: | Бюллетень сибирской медицины |
| Subjects: | |
| Online Access: | https://bulletin.ssmu.ru/jour/article/view/739 |
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| author | S. N. Orlov L. V. Smagliy S. V. Gusakova V. S. Rydchenko Yu. G. Birulina A. N. Baikov V. N. Vasiliyev G. A. Sukhanova T. S. Fedorova T. V. Lasukova |
| author_facet | S. N. Orlov L. V. Smagliy S. V. Gusakova V. S. Rydchenko Yu. G. Birulina A. N. Baikov V. N. Vasiliyev G. A. Sukhanova T. S. Fedorova T. V. Lasukova |
| author_sort | S. N. Orlov |
| collection | DOAJ |
| description | The purpose of this work is to study the influence of extracellular ATP (adenosine-3-phosphate), which is an activator of purinergic receptors, on contractive activity of rat aortic ring segments precontracted by α1-adrenoreceptors activation with phenylephrine and evaluate the impact of potassium channels of plasma membrane on mechanisms of ATP activity.Material and methods. Contractive activity of vascular smooth muscle cells was studied using the method of Organ Bath Myography applied to the thoracic aorta segments in male Wistar rats both with intact and removed endothelium. ATP (1–1000 mM) produced a dose-dependent relaxing effect on intact and endothelium-denuded segments precontracted with phenilephrine. To assess the impact of potassium channels on mechanisms of ATP activity we used tetraethylammonium (10 mM), a nonselective potassium-channel blocker, glibenclamide (10 mM), the blocker of ATP-sensitive potassium channels and 4-aminopiridine, a blocker of voltage-gated potassium channels.Conclusion. Our study has shown that the impact of ATP on segments with intact endothelium depends on the ATP-sensitive potassium channels whereas the impact of ATP on endothelium-denuded aortic segments depends on both ATP-sensitive and voltage-gated potassium channels. |
| first_indexed | 2024-04-10T01:07:55Z |
| format | Article |
| id | doaj.art-ce9df3fdb393468297d14999a6f15cd3 |
| institution | Directory Open Access Journal |
| issn | 1682-0363 1819-3684 |
| language | English |
| last_indexed | 2024-04-10T01:07:55Z |
| publishDate | 2017-01-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj.art-ce9df3fdb393468297d14999a6f15cd32023-03-13T09:58:22ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842017-01-0115510511210.20538/1682-0363-2016-5-105-112606Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cellsS. N. Orlov0L. V. Smagliy1S. V. Gusakova2V. S. Rydchenko3Yu. G. Birulina4A. N. Baikov5V. N. Vasiliyev6G. A. Sukhanova7T. S. Fedorova8T. V. Lasukova9Сибирский государственный медицинский университет; Московский государственный университет им. М.В. ЛомоносоваСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетТомский государственный педагогический университет (ТГПУ)The purpose of this work is to study the influence of extracellular ATP (adenosine-3-phosphate), which is an activator of purinergic receptors, on contractive activity of rat aortic ring segments precontracted by α1-adrenoreceptors activation with phenylephrine and evaluate the impact of potassium channels of plasma membrane on mechanisms of ATP activity.Material and methods. Contractive activity of vascular smooth muscle cells was studied using the method of Organ Bath Myography applied to the thoracic aorta segments in male Wistar rats both with intact and removed endothelium. ATP (1–1000 mM) produced a dose-dependent relaxing effect on intact and endothelium-denuded segments precontracted with phenilephrine. To assess the impact of potassium channels on mechanisms of ATP activity we used tetraethylammonium (10 mM), a nonselective potassium-channel blocker, glibenclamide (10 mM), the blocker of ATP-sensitive potassium channels and 4-aminopiridine, a blocker of voltage-gated potassium channels.Conclusion. Our study has shown that the impact of ATP on segments with intact endothelium depends on the ATP-sensitive potassium channels whereas the impact of ATP on endothelium-denuded aortic segments depends on both ATP-sensitive and voltage-gated potassium channels.https://bulletin.ssmu.ru/jour/article/view/739атфгладкомышечные клеткикалиевые каналы |
| spellingShingle | S. N. Orlov L. V. Smagliy S. V. Gusakova V. S. Rydchenko Yu. G. Birulina A. N. Baikov V. N. Vasiliyev G. A. Sukhanova T. S. Fedorova T. V. Lasukova Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells Бюллетень сибирской медицины атф гладкомышечные клетки калиевые каналы |
| title | Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells |
| title_full | Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells |
| title_fullStr | Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells |
| title_full_unstemmed | Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells |
| title_short | Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells |
| title_sort | role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells |
| topic | атф гладкомышечные клетки калиевые каналы |
| url | https://bulletin.ssmu.ru/jour/article/view/739 |
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