Enzymatic Hydrolysis of Textile and Cardboard Waste as a Glucose Source for the Production of Limonene in <i>Escherichia coli</i>
Cellulose containing textiles (cotton) and cardboard/carton waste represent a large reservoir of untapped organic carbon. These wastes have enormous potential for use as carbon feedstock in industrial biotechnological processes. Essentially, cotton/cardboard (CC) waste is pure cellulose (with some a...
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MDPI AG
2022-09-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/12/9/1423 |
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| author | Žiga Zebec Mojca Poberžnik Aleksandra Lobnik |
| author_facet | Žiga Zebec Mojca Poberžnik Aleksandra Lobnik |
| author_sort | Žiga Zebec |
| collection | DOAJ |
| description | Cellulose containing textiles (cotton) and cardboard/carton waste represent a large reservoir of untapped organic carbon. These wastes have enormous potential for use as carbon feedstock in industrial biotechnological processes. Essentially, cotton/cardboard (CC) waste is pure cellulose (with some additives) in the form of polymerised glucose consisting of β-(1→4)-linked D-glucose subunits. One of the largest and most diverse classes of natural chemicals that can be produced from glucose are terpenes with a wide range of applications as flavours, fragrances, pharmaceuticals, biopesticides, and biofuels. Here we have investigated the bioconversion of CC waste into the exemplary terpene limonene as a proof of concept. Six different CC waste streams were enzymatically hydrolysed and used to produce limonene using the <i>Escherichia coli</i> (<i>E. coli</i>) microbial cell factory. The D-glucose content in the CC hydrolysate (glucose juice) was determined and then metabolised by <i>E. coli</i> via a manipulated heterogeneous biolipid synthesis pathway (the mevalonate pathway) to produce limonene. This study represents an important proof of concept for the production of terpenes from hydrolysed CC waste streams. |
| first_indexed | 2024-03-09T23:22:51Z |
| format | Article |
| id | doaj.art-ce9dfc9bb4714a3e9a803c2aae47928f |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T23:22:51Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-ce9dfc9bb4714a3e9a803c2aae47928f2023-11-23T17:23:54ZengMDPI AGLife2075-17292022-09-01129142310.3390/life12091423Enzymatic Hydrolysis of Textile and Cardboard Waste as a Glucose Source for the Production of Limonene in <i>Escherichia coli</i>Žiga Zebec0Mojca Poberžnik1Aleksandra Lobnik2Faculty of Mechanical Engineering, University of Maribor, Smetanova Ul. 17, 2000 Maribor, SloveniaIOS d.o.o., Beloruska Ul. 7, 2000 Maribor, SloveniaFaculty of Mechanical Engineering, University of Maribor, Smetanova Ul. 17, 2000 Maribor, SloveniaCellulose containing textiles (cotton) and cardboard/carton waste represent a large reservoir of untapped organic carbon. These wastes have enormous potential for use as carbon feedstock in industrial biotechnological processes. Essentially, cotton/cardboard (CC) waste is pure cellulose (with some additives) in the form of polymerised glucose consisting of β-(1→4)-linked D-glucose subunits. One of the largest and most diverse classes of natural chemicals that can be produced from glucose are terpenes with a wide range of applications as flavours, fragrances, pharmaceuticals, biopesticides, and biofuels. Here we have investigated the bioconversion of CC waste into the exemplary terpene limonene as a proof of concept. Six different CC waste streams were enzymatically hydrolysed and used to produce limonene using the <i>Escherichia coli</i> (<i>E. coli</i>) microbial cell factory. The D-glucose content in the CC hydrolysate (glucose juice) was determined and then metabolised by <i>E. coli</i> via a manipulated heterogeneous biolipid synthesis pathway (the mevalonate pathway) to produce limonene. This study represents an important proof of concept for the production of terpenes from hydrolysed CC waste streams.https://www.mdpi.com/2075-1729/12/9/1423cotton/cardboard (CC) wasteenzymatic hydrolysisglucose juicesynthetic biologylimonene |
| spellingShingle | Žiga Zebec Mojca Poberžnik Aleksandra Lobnik Enzymatic Hydrolysis of Textile and Cardboard Waste as a Glucose Source for the Production of Limonene in <i>Escherichia coli</i> Life cotton/cardboard (CC) waste enzymatic hydrolysis glucose juice synthetic biology limonene |
| title | Enzymatic Hydrolysis of Textile and Cardboard Waste as a Glucose Source for the Production of Limonene in <i>Escherichia coli</i> |
| title_full | Enzymatic Hydrolysis of Textile and Cardboard Waste as a Glucose Source for the Production of Limonene in <i>Escherichia coli</i> |
| title_fullStr | Enzymatic Hydrolysis of Textile and Cardboard Waste as a Glucose Source for the Production of Limonene in <i>Escherichia coli</i> |
| title_full_unstemmed | Enzymatic Hydrolysis of Textile and Cardboard Waste as a Glucose Source for the Production of Limonene in <i>Escherichia coli</i> |
| title_short | Enzymatic Hydrolysis of Textile and Cardboard Waste as a Glucose Source for the Production of Limonene in <i>Escherichia coli</i> |
| title_sort | enzymatic hydrolysis of textile and cardboard waste as a glucose source for the production of limonene in i escherichia coli i |
| topic | cotton/cardboard (CC) waste enzymatic hydrolysis glucose juice synthetic biology limonene |
| url | https://www.mdpi.com/2075-1729/12/9/1423 |
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