Effects of high-fiber food on gut microbiology and energy metabolism in Eothenomys miletus at different altitudes
Intestinal microorganisms assist the host in digesting complex and difficultly decomposed foods; expand the host’s dietary ecological niche. In order to investigate the effect of high-fiber food on intestinal microorganisms of Eothenomys miletus at different altitudes, exploring the regional differe...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1264109/full |
| _version_ | 1797730767550283776 |
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| author | Wei Zhang Ting Jia Hao Zhang Wanlong Zhu Wanlong Zhu Wanlong Zhu |
| author_facet | Wei Zhang Ting Jia Hao Zhang Wanlong Zhu Wanlong Zhu Wanlong Zhu |
| author_sort | Wei Zhang |
| collection | DOAJ |
| description | Intestinal microorganisms assist the host in digesting complex and difficultly decomposed foods; expand the host’s dietary ecological niche. In order to investigate the effect of high-fiber food on intestinal microorganisms of Eothenomys miletus at different altitudes, exploring the regional differences of intestinal microorganisms and their roles in body mass regulation, we collected E. miletus from Dali (DL) and Xianggelila (XGLL), which were divided into control group, high-fiber group fed with high-fiber diet for 7 days, and refeeding group fed with standard diet for 14 days after high-fiber diet. Using 16S rRNA gene sequencing technology combined with physiological methods, we analyzed the gut microbial diversity, abundance, community structure and related physiological indicators of each group, and explored the effects of high-fiber foods and regions on the diversity, structure of gut microorganisms and physiological indicators. The results showed that high-fiber food affected the food intake and metabolic rate of E. miletus, which also showed regional differences. The intestinal microorganisms of E. miletus obtained energy through the enrichment of fiber degrading bacteria under the condition of high-fiber food, while producing short-chain fatty acids, which participated in processes such as energy metabolism or immune regulation. Moreover, it also affected the colonization of intestinal microorganisms. High-fiber food promoted the enrichment of probiotics in the intestinal microbiota of E. miletus, but pathogenic bacteria also appeared. Therefore, the changes in the composition and diversity of gut microbiota in E. miletus provided important guarantees for their adaptation to high fiber food environments in winter. |
| first_indexed | 2024-03-12T11:49:02Z |
| format | Article |
| id | doaj.art-ce9edc7a671842d38268697826e8866c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T11:49:02Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ce9edc7a671842d38268697826e8866c2023-08-31T11:40:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-08-011410.3389/fmicb.2023.12641091264109Effects of high-fiber food on gut microbiology and energy metabolism in Eothenomys miletus at different altitudesWei Zhang0Ting Jia1Hao Zhang2Wanlong Zhu3Wanlong Zhu4Wanlong Zhu5Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of Yunnan Province Higher Institutes College, School of Life Sciences, Yunnan Normal University, Kunming, ChinaKey Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of Yunnan Province Higher Institutes College, School of Life Sciences, Yunnan Normal University, Kunming, ChinaKey Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of Yunnan Province Higher Institutes College, School of Life Sciences, Yunnan Normal University, Kunming, ChinaKey Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of Yunnan Province Higher Institutes College, School of Life Sciences, Yunnan Normal University, Kunming, ChinaEngineering Research Center of Sustainable Development and Utilization of Biomass Energy Ministry of Education, Kunming, ChinaKey Laboratory of Yunnan Province for Biomass Energy and Environment Biotechnology, Kunming, ChinaIntestinal microorganisms assist the host in digesting complex and difficultly decomposed foods; expand the host’s dietary ecological niche. In order to investigate the effect of high-fiber food on intestinal microorganisms of Eothenomys miletus at different altitudes, exploring the regional differences of intestinal microorganisms and their roles in body mass regulation, we collected E. miletus from Dali (DL) and Xianggelila (XGLL), which were divided into control group, high-fiber group fed with high-fiber diet for 7 days, and refeeding group fed with standard diet for 14 days after high-fiber diet. Using 16S rRNA gene sequencing technology combined with physiological methods, we analyzed the gut microbial diversity, abundance, community structure and related physiological indicators of each group, and explored the effects of high-fiber foods and regions on the diversity, structure of gut microorganisms and physiological indicators. The results showed that high-fiber food affected the food intake and metabolic rate of E. miletus, which also showed regional differences. The intestinal microorganisms of E. miletus obtained energy through the enrichment of fiber degrading bacteria under the condition of high-fiber food, while producing short-chain fatty acids, which participated in processes such as energy metabolism or immune regulation. Moreover, it also affected the colonization of intestinal microorganisms. High-fiber food promoted the enrichment of probiotics in the intestinal microbiota of E. miletus, but pathogenic bacteria also appeared. Therefore, the changes in the composition and diversity of gut microbiota in E. miletus provided important guarantees for their adaptation to high fiber food environments in winter.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1264109/fullEothenomys miletusintestinal microbiotahigh-fiber foodsbody mass regulationadaptation |
| spellingShingle | Wei Zhang Ting Jia Hao Zhang Wanlong Zhu Wanlong Zhu Wanlong Zhu Effects of high-fiber food on gut microbiology and energy metabolism in Eothenomys miletus at different altitudes Frontiers in Microbiology Eothenomys miletus intestinal microbiota high-fiber foods body mass regulation adaptation |
| title | Effects of high-fiber food on gut microbiology and energy metabolism in Eothenomys miletus at different altitudes |
| title_full | Effects of high-fiber food on gut microbiology and energy metabolism in Eothenomys miletus at different altitudes |
| title_fullStr | Effects of high-fiber food on gut microbiology and energy metabolism in Eothenomys miletus at different altitudes |
| title_full_unstemmed | Effects of high-fiber food on gut microbiology and energy metabolism in Eothenomys miletus at different altitudes |
| title_short | Effects of high-fiber food on gut microbiology and energy metabolism in Eothenomys miletus at different altitudes |
| title_sort | effects of high fiber food on gut microbiology and energy metabolism in eothenomys miletus at different altitudes |
| topic | Eothenomys miletus intestinal microbiota high-fiber foods body mass regulation adaptation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1264109/full |
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