Biosignatures for Parkinson's disease and atypical parkinsonian disorders patients.
Diagnosis of Parkinson' disease (PD) carries a high misdiagnosis rate due to failure to recognize atypical parkinsonian disorders (APD). Usually by the time of diagnosis greater than 60% of the neurons in the substantia nigra are dead. Therefore, early detection would be beneficial so that ther...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22952715/pdf/?tool=EBI |
| _version_ | 1818742608371908608 |
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| author | Judith A Potashkin Jose A Santiago Bernard M Ravina Arthur Watts Alexey A Leontovich |
| author_facet | Judith A Potashkin Jose A Santiago Bernard M Ravina Arthur Watts Alexey A Leontovich |
| author_sort | Judith A Potashkin |
| collection | DOAJ |
| description | Diagnosis of Parkinson' disease (PD) carries a high misdiagnosis rate due to failure to recognize atypical parkinsonian disorders (APD). Usually by the time of diagnosis greater than 60% of the neurons in the substantia nigra are dead. Therefore, early detection would be beneficial so that therapeutic intervention may be initiated early in the disease process. We used splice variant-specific microarrays to identify mRNAs whose expression is altered in peripheral blood of early-stage PD patients compared to healthy and neurodegenerative disease controls. Quantitative polymerase chain reaction assays were used to validate splice variant transcripts in independent sample sets. Here we report a PD signature used to classify blinded samples with 90% sensitivity and 94% specificity and an APD signature that resulted in a diagnosis with 95% sensitivity and 94% specificity. This study provides the first discriminant functions with coherent diagnostic signatures for PD and APD. Analysis of the PD biomarkers identified a regulatory network with nodes centered on the transcription factors HNF4A and TNF, which have been implicated in insulin regulation. |
| first_indexed | 2024-12-18T02:15:13Z |
| format | Article |
| id | doaj.art-cea12965f5354884b9830decd12e6ed5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-18T02:15:13Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cea12965f5354884b9830decd12e6ed52022-12-21T21:24:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0178e4359510.1371/journal.pone.0043595Biosignatures for Parkinson's disease and atypical parkinsonian disorders patients.Judith A PotashkinJose A SantiagoBernard M RavinaArthur WattsAlexey A LeontovichDiagnosis of Parkinson' disease (PD) carries a high misdiagnosis rate due to failure to recognize atypical parkinsonian disorders (APD). Usually by the time of diagnosis greater than 60% of the neurons in the substantia nigra are dead. Therefore, early detection would be beneficial so that therapeutic intervention may be initiated early in the disease process. We used splice variant-specific microarrays to identify mRNAs whose expression is altered in peripheral blood of early-stage PD patients compared to healthy and neurodegenerative disease controls. Quantitative polymerase chain reaction assays were used to validate splice variant transcripts in independent sample sets. Here we report a PD signature used to classify blinded samples with 90% sensitivity and 94% specificity and an APD signature that resulted in a diagnosis with 95% sensitivity and 94% specificity. This study provides the first discriminant functions with coherent diagnostic signatures for PD and APD. Analysis of the PD biomarkers identified a regulatory network with nodes centered on the transcription factors HNF4A and TNF, which have been implicated in insulin regulation.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22952715/pdf/?tool=EBI |
| spellingShingle | Judith A Potashkin Jose A Santiago Bernard M Ravina Arthur Watts Alexey A Leontovich Biosignatures for Parkinson's disease and atypical parkinsonian disorders patients. PLoS ONE |
| title | Biosignatures for Parkinson's disease and atypical parkinsonian disorders patients. |
| title_full | Biosignatures for Parkinson's disease and atypical parkinsonian disorders patients. |
| title_fullStr | Biosignatures for Parkinson's disease and atypical parkinsonian disorders patients. |
| title_full_unstemmed | Biosignatures for Parkinson's disease and atypical parkinsonian disorders patients. |
| title_short | Biosignatures for Parkinson's disease and atypical parkinsonian disorders patients. |
| title_sort | biosignatures for parkinson s disease and atypical parkinsonian disorders patients |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22952715/pdf/?tool=EBI |
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