Evaluation of Two Serological Assays for Diagnosing Zika Virus Infection
Zika virus (ZIKV) emerged and spread rapidly in South American countries during 2015. Efforts to diagnose ZIKV infection using serological tools were challenging in dengue-endemic areas because of antigenic similarities between both viruses. Here, we assessed the performance of an in-house developed...
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MDPI AG
2021-09-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/11/9/1696 |
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| author | Steev Loyola Alfredo Huaman Dina Popuche Elizabeth Castillo Julia S. Ampuero Maria Silva Carolina Guevara Douglas M. Watts |
| author_facet | Steev Loyola Alfredo Huaman Dina Popuche Elizabeth Castillo Julia S. Ampuero Maria Silva Carolina Guevara Douglas M. Watts |
| author_sort | Steev Loyola |
| collection | DOAJ |
| description | Zika virus (ZIKV) emerged and spread rapidly in South American countries during 2015. Efforts to diagnose ZIKV infection using serological tools were challenging in dengue-endemic areas because of antigenic similarities between both viruses. Here, we assessed the performance of an in-house developed IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) and the plaque reduction neutralization test (PRNT) to diagnose ZIKV infection. Acute and convalescent paired serum samples from 51 patients who presented with clinical symptoms suggestive of an arbovirus illness in dengue-endemic areas of Honduras, Venezuela, Colombia and Peru were used in the assessment. Samples were tested for ZIKV, dengue and chikungunya virus using a variety of laboratory techniques. The results for the ZIKV-RNA screening and seroconversion detected by the microneutralization test were used to construct a composite reference standard. The overall sensitivity and specificity for the MAC-ELISA were 93.5% and 100.0%, respectively. Contrastingly, the overall sensitivity and specificity for the PRNT were 96.8% and 95.0%, respectively. Restricting the analysis according to IgM or neutralizing antibodies against dengue, the performances of both serological assays were adequate. The findings of this study reveal that the MAC-ELISA and PRNT would provide initial reliable laboratory diagnostic assays for ZIKV infection in dengue-endemic areas. |
| first_indexed | 2024-03-10T07:45:52Z |
| format | Article |
| id | doaj.art-cea2b9219beb4adaa5d2c1f8f1073d66 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T07:45:52Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-cea2b9219beb4adaa5d2c1f8f1073d662023-11-22T12:40:59ZengMDPI AGDiagnostics2075-44182021-09-01119169610.3390/diagnostics11091696Evaluation of Two Serological Assays for Diagnosing Zika Virus InfectionSteev Loyola0Alfredo Huaman1Dina Popuche2Elizabeth Castillo3Julia S. Ampuero4Maria Silva5Carolina Guevara6Douglas M. Watts7U.S. Naval Medical Research Unit No. 6 (NAMRU-6), Lima 07006, PeruU.S. Naval Medical Research Unit No. 6 (NAMRU-6), Lima 07006, PeruU.S. Naval Medical Research Unit No. 6 (NAMRU-6), Lima 07006, PeruU.S. Naval Medical Research Unit No. 6 (NAMRU-6), Lima 07006, PeruU.S. Naval Medical Research Unit No. 6 (NAMRU-6), Lima 07006, PeruU.S. Naval Medical Research Unit No. 6 (NAMRU-6), Lima 07006, PeruU.S. Naval Medical Research Unit No. 6 (NAMRU-6), Lima 07006, PeruDepartment of Biological Sciences, University of Texas at El Paso, El Paso, TX 79968, USAZika virus (ZIKV) emerged and spread rapidly in South American countries during 2015. Efforts to diagnose ZIKV infection using serological tools were challenging in dengue-endemic areas because of antigenic similarities between both viruses. Here, we assessed the performance of an in-house developed IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) and the plaque reduction neutralization test (PRNT) to diagnose ZIKV infection. Acute and convalescent paired serum samples from 51 patients who presented with clinical symptoms suggestive of an arbovirus illness in dengue-endemic areas of Honduras, Venezuela, Colombia and Peru were used in the assessment. Samples were tested for ZIKV, dengue and chikungunya virus using a variety of laboratory techniques. The results for the ZIKV-RNA screening and seroconversion detected by the microneutralization test were used to construct a composite reference standard. The overall sensitivity and specificity for the MAC-ELISA were 93.5% and 100.0%, respectively. Contrastingly, the overall sensitivity and specificity for the PRNT were 96.8% and 95.0%, respectively. Restricting the analysis according to IgM or neutralizing antibodies against dengue, the performances of both serological assays were adequate. The findings of this study reveal that the MAC-ELISA and PRNT would provide initial reliable laboratory diagnostic assays for ZIKV infection in dengue-endemic areas.https://www.mdpi.com/2075-4418/11/9/1696Zika virusenzyme-linked immunosorbent assayneutralization tests |
| spellingShingle | Steev Loyola Alfredo Huaman Dina Popuche Elizabeth Castillo Julia S. Ampuero Maria Silva Carolina Guevara Douglas M. Watts Evaluation of Two Serological Assays for Diagnosing Zika Virus Infection Diagnostics Zika virus enzyme-linked immunosorbent assay neutralization tests |
| title | Evaluation of Two Serological Assays for Diagnosing Zika Virus Infection |
| title_full | Evaluation of Two Serological Assays for Diagnosing Zika Virus Infection |
| title_fullStr | Evaluation of Two Serological Assays for Diagnosing Zika Virus Infection |
| title_full_unstemmed | Evaluation of Two Serological Assays for Diagnosing Zika Virus Infection |
| title_short | Evaluation of Two Serological Assays for Diagnosing Zika Virus Infection |
| title_sort | evaluation of two serological assays for diagnosing zika virus infection |
| topic | Zika virus enzyme-linked immunosorbent assay neutralization tests |
| url | https://www.mdpi.com/2075-4418/11/9/1696 |
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