Optimization of the CYP inhibition assay using LC-MS/MS
The data presented in this article are related to the research article entitled “Cytochrome P450 inhibition activities of non-standardized botanical products” [1], in which the possible CYP inhibitory properties of botanical products were investigated. This article describes the optimization and bio...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | MethodsX |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016122002072 |
| _version_ | 1811201278295932928 |
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| author | Muhammad Asyraf Abduraman Nor Hidayah Mustafa Nik Soriani Yaacob Azimah Amanah Mei Lan Tan |
| author_facet | Muhammad Asyraf Abduraman Nor Hidayah Mustafa Nik Soriani Yaacob Azimah Amanah Mei Lan Tan |
| author_sort | Muhammad Asyraf Abduraman |
| collection | DOAJ |
| description | The data presented in this article are related to the research article entitled “Cytochrome P450 inhibition activities of non-standardized botanical products” [1], in which the possible CYP inhibitory properties of botanical products were investigated. This article describes the optimization and bioanalytical method validation of the CYP (Cytochrome P450 inhibition assay) inhibition assays, namely, phenacetin O-deethylase assay, testosterone 6β-hydroxylase assay, felodipine dehydrogenase assay and midazolam 1’-hydroxylase assay using LC-MS/MS. |
| first_indexed | 2024-04-12T02:19:25Z |
| format | Article |
| id | doaj.art-cea3a781c5f447be9e2d85f3770d2e3f |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-04-12T02:19:25Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-cea3a781c5f447be9e2d85f3770d2e3f2022-12-22T03:52:10ZengElsevierMethodsX2215-01612022-01-019101827Optimization of the CYP inhibition assay using LC-MS/MSMuhammad Asyraf Abduraman0Nor Hidayah Mustafa1Nik Soriani Yaacob2Azimah Amanah3Mei Lan Tan4School of Pharmaceutical Sciences, Universiti Sains Malaysia, Pulau Pinang 11800, MalaysiaDrug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, MalaysiaSchool of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan 16150, MalaysiaMalaysian Institute of Pharmaceuticals & Nutraceuticals (IPharm), National Institutes of Biotechnology Malaysia (NIBM), Pulau Pinang 11700, MalaysiaSchool of Pharmaceutical Sciences, Universiti Sains Malaysia, Pulau Pinang 11800, Malaysia; Corresponding author.The data presented in this article are related to the research article entitled “Cytochrome P450 inhibition activities of non-standardized botanical products” [1], in which the possible CYP inhibitory properties of botanical products were investigated. This article describes the optimization and bioanalytical method validation of the CYP (Cytochrome P450 inhibition assay) inhibition assays, namely, phenacetin O-deethylase assay, testosterone 6β-hydroxylase assay, felodipine dehydrogenase assay and midazolam 1’-hydroxylase assay using LC-MS/MS.http://www.sciencedirect.com/science/article/pii/S2215016122002072Cytochrome P450 inhibition assay |
| spellingShingle | Muhammad Asyraf Abduraman Nor Hidayah Mustafa Nik Soriani Yaacob Azimah Amanah Mei Lan Tan Optimization of the CYP inhibition assay using LC-MS/MS MethodsX Cytochrome P450 inhibition assay |
| title | Optimization of the CYP inhibition assay using LC-MS/MS |
| title_full | Optimization of the CYP inhibition assay using LC-MS/MS |
| title_fullStr | Optimization of the CYP inhibition assay using LC-MS/MS |
| title_full_unstemmed | Optimization of the CYP inhibition assay using LC-MS/MS |
| title_short | Optimization of the CYP inhibition assay using LC-MS/MS |
| title_sort | optimization of the cyp inhibition assay using lc ms ms |
| topic | Cytochrome P450 inhibition assay |
| url | http://www.sciencedirect.com/science/article/pii/S2215016122002072 |
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