Calcium imaging with genetically encoded indicators in behaving primates
Understanding the neural basis of behaviour requires studying brain activity in behaving subjects using complementary techniques that measure neural responses at multiple spatial scales, and developing computational tools for understanding the mapping between these measurements. Here we report the f...
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2016-07-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/16178 |
| _version_ | 1811200200106049536 |
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| author | Eyal Seidemann Yuzhi Chen Yoon Bai Spencer C Chen Preeti Mehta Bridget L Kajs Wilson S Geisler Boris V Zemelman |
| author_facet | Eyal Seidemann Yuzhi Chen Yoon Bai Spencer C Chen Preeti Mehta Bridget L Kajs Wilson S Geisler Boris V Zemelman |
| author_sort | Eyal Seidemann |
| collection | DOAJ |
| description | Understanding the neural basis of behaviour requires studying brain activity in behaving subjects using complementary techniques that measure neural responses at multiple spatial scales, and developing computational tools for understanding the mapping between these measurements. Here we report the first results of widefield imaging of genetically encoded calcium indicator (GCaMP6f) signals from V1 of behaving macaques. This technique provides a robust readout of visual population responses at the columnar scale over multiple mm2 and over several months. To determine the quantitative relation between the widefield GCaMP signals and the locally pooled spiking activity, we developed a computational model that sums the responses of V1 neurons characterized by prior single unit measurements. The measured tuning properties of the GCaMP signals to stimulus contrast, orientation and spatial position closely match the predictions of the model, suggesting that widefield GCaMP signals are linearly related to the summed local spiking activity. |
| first_indexed | 2024-04-12T01:59:38Z |
| format | Article |
| id | doaj.art-cea590d4fa74453cb0e8507ff5135dfb |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:59:38Z |
| publishDate | 2016-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-cea590d4fa74453cb0e8507ff5135dfb2022-12-22T03:52:42ZengeLife Sciences Publications LtdeLife2050-084X2016-07-01510.7554/eLife.16178Calcium imaging with genetically encoded indicators in behaving primatesEyal Seidemann0https://orcid.org/0000-0003-2841-5948Yuzhi Chen1Yoon Bai2Spencer C Chen3Preeti Mehta4Bridget L Kajs5Wilson S Geisler6Boris V Zemelman7Center for Perceptual Systems, University of Texas, Austin, United States; Department of Psychology, University of Texas, Austin, United States; Department of Neuroscience, University of Texas, Austin, United StatesCenter for Perceptual Systems, University of Texas, Austin, United States; Department of Psychology, University of Texas, Austin, United States; Department of Neuroscience, University of Texas, Austin, United StatesCenter for Perceptual Systems, University of Texas, Austin, United States; Department of Psychology, University of Texas, Austin, United States; Department of Neuroscience, University of Texas, Austin, United StatesCenter for Perceptual Systems, University of Texas, Austin, United States; Department of Psychology, University of Texas, Austin, United States; Department of Neuroscience, University of Texas, Austin, United StatesDepartment of Neuroscience, University of Texas, Austin, United States; Center for Learning and Memory, University of Texas, Austin, United StatesDepartment of Neuroscience, University of Texas, Austin, United States; Center for Learning and Memory, University of Texas, Austin, United StatesCenter for Perceptual Systems, University of Texas, Austin, United States; Department of Psychology, University of Texas, Austin, United StatesDepartment of Neuroscience, University of Texas, Austin, United States; Center for Learning and Memory, University of Texas, Austin, United StatesUnderstanding the neural basis of behaviour requires studying brain activity in behaving subjects using complementary techniques that measure neural responses at multiple spatial scales, and developing computational tools for understanding the mapping between these measurements. Here we report the first results of widefield imaging of genetically encoded calcium indicator (GCaMP6f) signals from V1 of behaving macaques. This technique provides a robust readout of visual population responses at the columnar scale over multiple mm2 and over several months. To determine the quantitative relation between the widefield GCaMP signals and the locally pooled spiking activity, we developed a computational model that sums the responses of V1 neurons characterized by prior single unit measurements. The measured tuning properties of the GCaMP signals to stimulus contrast, orientation and spatial position closely match the predictions of the model, suggesting that widefield GCaMP signals are linearly related to the summed local spiking activity.https://elifesciences.org/articles/16178behaving macaquecalcium indicatorwidefield imaging |
| spellingShingle | Eyal Seidemann Yuzhi Chen Yoon Bai Spencer C Chen Preeti Mehta Bridget L Kajs Wilson S Geisler Boris V Zemelman Calcium imaging with genetically encoded indicators in behaving primates eLife behaving macaque calcium indicator widefield imaging |
| title | Calcium imaging with genetically encoded indicators in behaving primates |
| title_full | Calcium imaging with genetically encoded indicators in behaving primates |
| title_fullStr | Calcium imaging with genetically encoded indicators in behaving primates |
| title_full_unstemmed | Calcium imaging with genetically encoded indicators in behaving primates |
| title_short | Calcium imaging with genetically encoded indicators in behaving primates |
| title_sort | calcium imaging with genetically encoded indicators in behaving primates |
| topic | behaving macaque calcium indicator widefield imaging |
| url | https://elifesciences.org/articles/16178 |
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