New Kid on the Block: The Efficacy of Phytomedicine Extracts Urox® in Reducing Overactive Bladder Symptoms in Rats
The aim of the current study was to determine if phytomedicine (Urox®) would reverse retinyl acetate (RA)–induced changes characteristic of bladder overactivity. There were 60 rats divided into the following 4 groups: I—control, II—received RA to induce detrusor overactivity (DO), III—received Urox...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2022.896624/full |
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| author | Łukasz Zapała Kajetan Juszczak Przemysław Adamczyk Jan Adamowicz Aleksander Ślusarczyk Tomasz Kluz Marcin Misiek Artur Rogowski Magdalena Emilia Grzybowska Klaudia Stangel-Wójcikiewicz Mikołaj Piotr Zaborowski Ewa Poleszak Piotr Radziszewski Andrzej Wróbel |
| author_facet | Łukasz Zapała Kajetan Juszczak Przemysław Adamczyk Jan Adamowicz Aleksander Ślusarczyk Tomasz Kluz Marcin Misiek Artur Rogowski Magdalena Emilia Grzybowska Klaudia Stangel-Wójcikiewicz Mikołaj Piotr Zaborowski Ewa Poleszak Piotr Radziszewski Andrzej Wróbel |
| author_sort | Łukasz Zapała |
| collection | DOAJ |
| description | The aim of the current study was to determine if phytomedicine (Urox®) would reverse retinyl acetate (RA)–induced changes characteristic of bladder overactivity. There were 60 rats divided into the following 4 groups: I—control, II—received RA to induce detrusor overactivity (DO), III—received Urox (840 mg daily for 14 days), and IV—received combination of RA and Urox®. The cystometry was performed 2 days after the last dose of Urox®. Next, urothelium thickness and biochemical parameter measurements were performed. In group IV, a decrease in basal pressure and detrusor overactivity index was noted when compared to group II. Furthermore, in group IV the following parameters were increased: threshold pressure, voided volume, intercontraction interval, and bladder compliance in comparison with group II. There were significant elevations in c-Fos expression in the neuronal voiding centers in group II, while the expression of c-Fos in group IV was normalized. No significant changes in the values of the analyzed biomarkers in group III were found, while in group II, an elevation in BDNF, NGF, CGRP, ATP, Rho kinase, malondialdehyde, 3-nitrotyrosine, TRPV1, OCT-3, and VAChT and then a decrease in E-cadherin and Z01 were found. A successful restoration of all the abovementioned biomarkers’ levels was observed in group IV. Phytomedicine extracts (Urox®) were found to be potent in reversing RA-induced changes in several cystometric and biochemical parameters that are determinants of overactive bladder (OAB). The actions of Urox® were proved to be dependent on several factors, such as growth factors and several OAB biomarkers but not pro-inflammatory cytokines. |
| first_indexed | 2024-04-12T13:43:21Z |
| format | Article |
| id | doaj.art-cea8de62e4ec4e048a7228eddfb88e00 |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-04-12T13:43:21Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-cea8de62e4ec4e048a7228eddfb88e002022-12-22T03:30:47ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-06-01910.3389/fmolb.2022.896624896624New Kid on the Block: The Efficacy of Phytomedicine Extracts Urox® in Reducing Overactive Bladder Symptoms in RatsŁukasz Zapała0Kajetan Juszczak1Przemysław Adamczyk2Jan Adamowicz3Aleksander Ślusarczyk4Tomasz Kluz5Marcin Misiek6Artur Rogowski7Magdalena Emilia Grzybowska8Klaudia Stangel-Wójcikiewicz9Mikołaj Piotr Zaborowski10Ewa Poleszak11Piotr Radziszewski12Andrzej Wróbel13Clinic of General, Oncological and Functional Urology, Medical University of Warsaw, Warsaw, PolandChair of Urology and Andrology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Bydgoszcz, PolandDepartment of General and Oncologic Urology, Nicolaus Copernicus Hospital, Torun, PolandClinic of General and Oncologic Urology, Collegium Medicum of Nicolaus Copernicus University, Bydgoszcz, PolandClinic of General, Oncological and Functional Urology, Medical University of Warsaw, Warsaw, PolandDepartment of Gynecology, Gynecological Oncology and Obstetrics, Institute of Medical Sciences, Medical College of Rzeszow University, Rzeszow, PolandDepartment of Gynecologic Oncology, Holy Cross Cancer Center, Kielce, PolandDepartment of Minimally Invasive and Endoscopic Gynecology, Military Institute of Medicine, Legionowo Hospital, Legionowo, PolandDepartment of Gynecology, Gynecological Oncology and Gynecological Endocrinology, Medical University of Gdansk, Gdansk, PolandDepartment of Gynecology and Oncology, Jagiellonian University Medical College, Cracow, Poland0Department of Gynecology, Obstetrics and Gynecologic Oncology, Division of Gynecologic Oncology, Poznan University of Medical Sciences, Poznań, Poland1Laboratory of Preclinical Testing, Chair and Department of Applied and Social Pharmacy, Medical University of Lublin, Lublin, PolandClinic of General, Oncological and Functional Urology, Medical University of Warsaw, Warsaw, Poland2Second Department of Gynecology, Medical University of Lublin, Lublin, PolandThe aim of the current study was to determine if phytomedicine (Urox®) would reverse retinyl acetate (RA)–induced changes characteristic of bladder overactivity. There were 60 rats divided into the following 4 groups: I—control, II—received RA to induce detrusor overactivity (DO), III—received Urox (840 mg daily for 14 days), and IV—received combination of RA and Urox®. The cystometry was performed 2 days after the last dose of Urox®. Next, urothelium thickness and biochemical parameter measurements were performed. In group IV, a decrease in basal pressure and detrusor overactivity index was noted when compared to group II. Furthermore, in group IV the following parameters were increased: threshold pressure, voided volume, intercontraction interval, and bladder compliance in comparison with group II. There were significant elevations in c-Fos expression in the neuronal voiding centers in group II, while the expression of c-Fos in group IV was normalized. No significant changes in the values of the analyzed biomarkers in group III were found, while in group II, an elevation in BDNF, NGF, CGRP, ATP, Rho kinase, malondialdehyde, 3-nitrotyrosine, TRPV1, OCT-3, and VAChT and then a decrease in E-cadherin and Z01 were found. A successful restoration of all the abovementioned biomarkers’ levels was observed in group IV. Phytomedicine extracts (Urox®) were found to be potent in reversing RA-induced changes in several cystometric and biochemical parameters that are determinants of overactive bladder (OAB). The actions of Urox® were proved to be dependent on several factors, such as growth factors and several OAB biomarkers but not pro-inflammatory cytokines.https://www.frontiersin.org/articles/10.3389/fmolb.2022.896624/fullphytomedicineoveractive bladderUroxherbal drugsbiomarkers |
| spellingShingle | Łukasz Zapała Kajetan Juszczak Przemysław Adamczyk Jan Adamowicz Aleksander Ślusarczyk Tomasz Kluz Marcin Misiek Artur Rogowski Magdalena Emilia Grzybowska Klaudia Stangel-Wójcikiewicz Mikołaj Piotr Zaborowski Ewa Poleszak Piotr Radziszewski Andrzej Wróbel New Kid on the Block: The Efficacy of Phytomedicine Extracts Urox® in Reducing Overactive Bladder Symptoms in Rats Frontiers in Molecular Biosciences phytomedicine overactive bladder Urox herbal drugs biomarkers |
| title | New Kid on the Block: The Efficacy of Phytomedicine Extracts Urox® in Reducing Overactive Bladder Symptoms in Rats |
| title_full | New Kid on the Block: The Efficacy of Phytomedicine Extracts Urox® in Reducing Overactive Bladder Symptoms in Rats |
| title_fullStr | New Kid on the Block: The Efficacy of Phytomedicine Extracts Urox® in Reducing Overactive Bladder Symptoms in Rats |
| title_full_unstemmed | New Kid on the Block: The Efficacy of Phytomedicine Extracts Urox® in Reducing Overactive Bladder Symptoms in Rats |
| title_short | New Kid on the Block: The Efficacy of Phytomedicine Extracts Urox® in Reducing Overactive Bladder Symptoms in Rats |
| title_sort | new kid on the block the efficacy of phytomedicine extracts urox r in reducing overactive bladder symptoms in rats |
| topic | phytomedicine overactive bladder Urox herbal drugs biomarkers |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2022.896624/full |
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