Developmental Competence of Domestic Cat Vitrified Oocytes in 3D Enriched Culture Conditions
Cryoinjuries severely affect the competence of vitrified oocytes (VOs) to develop into embryos after warming. The use of culture conditions that provide physical and chemical support and resemble the in vivo microenvironment in which oocytes develop, such as 3D scaffolds and coculture systems, might...
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MDPI AG
2019-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/9/6/329 |
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| author | Martina Colombo Maria Giorgia Morselli Mariana Riboli Tavares Maricy Apparicio Gaia Cecilia Luvoni |
| author_facet | Martina Colombo Maria Giorgia Morselli Mariana Riboli Tavares Maricy Apparicio Gaia Cecilia Luvoni |
| author_sort | Martina Colombo |
| collection | DOAJ |
| description | Cryoinjuries severely affect the competence of vitrified oocytes (VOs) to develop into embryos after warming. The use of culture conditions that provide physical and chemical support and resemble the in vivo microenvironment in which oocytes develop, such as 3D scaffolds and coculture systems, might be useful to improve VOs outcomes. In this study, an enriched culture system of 3D barium alginate microcapsules was employed for the in vitro embryo production of domestic cat VOs. Cryotop vitrified-warmed oocytes were in vitro matured for 24 h in the 3D system with or without fresh cumulus-oocyte complexes (COCs) in coculture, whereas a control group of VOs was cultured in traditional 2D microdrops of medium. After in vitro fertilization, presumptive embryos were cultured in 3D or 2D systems according to the maturation conditions. Vitrified oocytes were able to mature and develop into embryos in 3D microcapsules (17.42 ± 11.83%) as well as in 2D microdrops (14.96 ± 8.80%), but the coculture with companion COCs in 3D resulted in similar proportions of VOs embryo development (18.39 ± 16.67%; <i>p</i> = 1.00), although COCs presence allowed for blastocyst formation (0.95 ± 2.52%). In conclusion, embryos until late developmental stages were obtained from cat VOs, and 3D microcapsules were comparable to 2D microdrops, but improvements in post-warming conditions are still needed. |
| first_indexed | 2024-12-22T01:24:04Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-12-22T01:24:04Z |
| publishDate | 2019-06-01 |
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| series | Animals |
| spelling | doaj.art-ceac405c49f845cfacb3fe3ed4da6d1d2022-12-21T18:43:39ZengMDPI AGAnimals2076-26152019-06-019632910.3390/ani9060329ani9060329Developmental Competence of Domestic Cat Vitrified Oocytes in 3D Enriched Culture ConditionsMartina Colombo0Maria Giorgia Morselli1Mariana Riboli Tavares2Maricy Apparicio3Gaia Cecilia Luvoni4Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare “Carlo Cantoni”, Università degli Studi di Milano, Via Celoria, 10, 20133 Milano, ItalyDipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare “Carlo Cantoni”, Università degli Studi di Milano, Via Celoria, 10, 20133 Milano, ItalyDepartamento de Medicina Veterinária Preventiva e Reprodução Animal, Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal 14884-900, BrazilDepartamento de Medicina Veterinária Preventiva e Reprodução Animal, Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal 14884-900, BrazilDipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare “Carlo Cantoni”, Università degli Studi di Milano, Via Celoria, 10, 20133 Milano, ItalyCryoinjuries severely affect the competence of vitrified oocytes (VOs) to develop into embryos after warming. The use of culture conditions that provide physical and chemical support and resemble the in vivo microenvironment in which oocytes develop, such as 3D scaffolds and coculture systems, might be useful to improve VOs outcomes. In this study, an enriched culture system of 3D barium alginate microcapsules was employed for the in vitro embryo production of domestic cat VOs. Cryotop vitrified-warmed oocytes were in vitro matured for 24 h in the 3D system with or without fresh cumulus-oocyte complexes (COCs) in coculture, whereas a control group of VOs was cultured in traditional 2D microdrops of medium. After in vitro fertilization, presumptive embryos were cultured in 3D or 2D systems according to the maturation conditions. Vitrified oocytes were able to mature and develop into embryos in 3D microcapsules (17.42 ± 11.83%) as well as in 2D microdrops (14.96 ± 8.80%), but the coculture with companion COCs in 3D resulted in similar proportions of VOs embryo development (18.39 ± 16.67%; <i>p</i> = 1.00), although COCs presence allowed for blastocyst formation (0.95 ± 2.52%). In conclusion, embryos until late developmental stages were obtained from cat VOs, and 3D microcapsules were comparable to 2D microdrops, but improvements in post-warming conditions are still needed.https://www.mdpi.com/2076-2615/9/6/329alginate microcapsuleblastocystcryopreservationfelinein vitro culturethree-dimensional |
| spellingShingle | Martina Colombo Maria Giorgia Morselli Mariana Riboli Tavares Maricy Apparicio Gaia Cecilia Luvoni Developmental Competence of Domestic Cat Vitrified Oocytes in 3D Enriched Culture Conditions Animals alginate microcapsule blastocyst cryopreservation feline in vitro culture three-dimensional |
| title | Developmental Competence of Domestic Cat Vitrified Oocytes in 3D Enriched Culture Conditions |
| title_full | Developmental Competence of Domestic Cat Vitrified Oocytes in 3D Enriched Culture Conditions |
| title_fullStr | Developmental Competence of Domestic Cat Vitrified Oocytes in 3D Enriched Culture Conditions |
| title_full_unstemmed | Developmental Competence of Domestic Cat Vitrified Oocytes in 3D Enriched Culture Conditions |
| title_short | Developmental Competence of Domestic Cat Vitrified Oocytes in 3D Enriched Culture Conditions |
| title_sort | developmental competence of domestic cat vitrified oocytes in 3d enriched culture conditions |
| topic | alginate microcapsule blastocyst cryopreservation feline in vitro culture three-dimensional |
| url | https://www.mdpi.com/2076-2615/9/6/329 |
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