Disentangling the abundance and structure of Vibrio communities in a semi-enclosed Bay with mariculture (Dongshan Bay, Southern China)
The genus Vibrio contains a diverse group of heterotrophic bacteria, which are members of ubiquitous and abundant microbial communities in coastal ecosystems. Vibrio has been frequently found in a wide range of marine environments either by employing Vibrio-specific 16S rRNA sequencing or culturing...
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| Language: | English |
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Elsevier
2021-01-01
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| Series: | Computational and Structural Biotechnology Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2001037021003305 |
| _version_ | 1818974415340175360 |
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| author | Wei Xu Wenzhen Lin Zhichao Wang Yuanhao Gao Yu Luo Hans-Peter Grossart Ying Guo Qiancheng Gao Lixing Huang Zhuhua Luo |
| author_facet | Wei Xu Wenzhen Lin Zhichao Wang Yuanhao Gao Yu Luo Hans-Peter Grossart Ying Guo Qiancheng Gao Lixing Huang Zhuhua Luo |
| author_sort | Wei Xu |
| collection | DOAJ |
| description | The genus Vibrio contains a diverse group of heterotrophic bacteria, which are members of ubiquitous and abundant microbial communities in coastal ecosystems. Vibrio has been frequently found in a wide range of marine environments either by employing Vibrio-specific 16S rRNA sequencing or culturing methods. A combination of molecular and cultivation-dependent methods was developed to more precisely discriminate between different members of the genus Vibrio in seawater. This newly developed assay was subsequently applied to characterize Vibrio community composition in surface water at 18 mariculture sites. It Substantially improved the taxonomic resolution of Vibrio species when compared to traditional 16S rRNA analysis. Our qPCR and cultivation analyses revealed that average Vibrio abundance (Vibrio 16S rRNA gene copy numbers: 3.46 × 106 to 6.70 × 106 copies L−1) and live cell numbers (5.65 × 104–5.75 × 105 cfu mL−1) are significantly related to pH. Total bacteria and Vibrio-specific 16S rRNA metabarcode sequenceing resulted in a total of 10 and 32 operational taxonomic units (OTUs), respectively, and 15 Vibrio species were identified by targeted cultivation of Vibrio strains, with Vibrio fortis and V. brasiliensis dominating in the mariculture areas. The purpose of this study was to combine several analytical methods to improve current sequence-based Vibrio community surveys, and to prove for the effectiveness of this methodological approach comprehensively testing for Vibrio dynamics in different coastal environments. |
| first_indexed | 2024-12-20T15:39:42Z |
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| id | doaj.art-ceac4a1a663d4486978cefa589512087 |
| institution | Directory Open Access Journal |
| issn | 2001-0370 |
| language | English |
| last_indexed | 2024-12-20T15:39:42Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Computational and Structural Biotechnology Journal |
| spelling | doaj.art-ceac4a1a663d4486978cefa5895120872022-12-21T19:35:16ZengElsevierComputational and Structural Biotechnology Journal2001-03702021-01-011943814393Disentangling the abundance and structure of Vibrio communities in a semi-enclosed Bay with mariculture (Dongshan Bay, Southern China)Wei Xu0Wenzhen Lin1Zhichao Wang2Yuanhao Gao3Yu Luo4Hans-Peter Grossart5Ying Guo6Qiancheng Gao7Lixing Huang8Zhuhua Luo9Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China; Fujian Key Laboratory of Subtropical Plant Physiology and Biochemistry, Fujian Institute of Subtropical Botany, Xiamen 361002, China; School of Advanced Manufacturing, Fuzhou University, Jinjiang 362200, ChinaFujian Key Laboratory of Subtropical Plant Physiology and Biochemistry, Fujian Institute of Subtropical Botany, Xiamen 361002, ChinaKey Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, ChinaKey Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, ChinaKey Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China; School of Advanced Manufacturing, Fuzhou University, Jinjiang 362200, ChinaDepartment of Experimental Limnology, Leibniz Institute of Freshwater Ecology and Inland Fisheries, Stechlin 16775, Germany; Institute of Biochemistry and Biology, Postdam University, Potsdam 14469, GermanyFujian Key Laboratory of Subtropical Plant Physiology and Biochemistry, Fujian Institute of Subtropical Botany, Xiamen 361002, ChinaFisheries College, Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Jimei University, Xiamen 361021, ChinaFisheries College, Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Jimei University, Xiamen 361021, China; Corresponding authors at: Fisheries College, Jimei University, 43 Yindou Street, Xiamen, Fujian 361021, China (L. Huang). Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, 178 Daxue Road, Xiamen 361005, China (Zhuhua Luo).Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China; School of Advanced Manufacturing, Fuzhou University, Jinjiang 362200, China; School of Marine Sciences, Nanjing University of Information Science & Technology, Nanjing 210044, China; Corresponding authors at: Fisheries College, Jimei University, 43 Yindou Street, Xiamen, Fujian 361021, China (L. Huang). Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, 178 Daxue Road, Xiamen 361005, China (Zhuhua Luo).The genus Vibrio contains a diverse group of heterotrophic bacteria, which are members of ubiquitous and abundant microbial communities in coastal ecosystems. Vibrio has been frequently found in a wide range of marine environments either by employing Vibrio-specific 16S rRNA sequencing or culturing methods. A combination of molecular and cultivation-dependent methods was developed to more precisely discriminate between different members of the genus Vibrio in seawater. This newly developed assay was subsequently applied to characterize Vibrio community composition in surface water at 18 mariculture sites. It Substantially improved the taxonomic resolution of Vibrio species when compared to traditional 16S rRNA analysis. Our qPCR and cultivation analyses revealed that average Vibrio abundance (Vibrio 16S rRNA gene copy numbers: 3.46 × 106 to 6.70 × 106 copies L−1) and live cell numbers (5.65 × 104–5.75 × 105 cfu mL−1) are significantly related to pH. Total bacteria and Vibrio-specific 16S rRNA metabarcode sequenceing resulted in a total of 10 and 32 operational taxonomic units (OTUs), respectively, and 15 Vibrio species were identified by targeted cultivation of Vibrio strains, with Vibrio fortis and V. brasiliensis dominating in the mariculture areas. The purpose of this study was to combine several analytical methods to improve current sequence-based Vibrio community surveys, and to prove for the effectiveness of this methodological approach comprehensively testing for Vibrio dynamics in different coastal environments.http://www.sciencedirect.com/science/article/pii/S200103702100330516S rRNACfuVibrio diversityVibrio abundanceMaricultureDongshan bay |
| spellingShingle | Wei Xu Wenzhen Lin Zhichao Wang Yuanhao Gao Yu Luo Hans-Peter Grossart Ying Guo Qiancheng Gao Lixing Huang Zhuhua Luo Disentangling the abundance and structure of Vibrio communities in a semi-enclosed Bay with mariculture (Dongshan Bay, Southern China) Computational and Structural Biotechnology Journal 16S rRNA Cfu Vibrio diversity Vibrio abundance Mariculture Dongshan bay |
| title | Disentangling the abundance and structure of Vibrio communities in a semi-enclosed Bay with mariculture (Dongshan Bay, Southern China) |
| title_full | Disentangling the abundance and structure of Vibrio communities in a semi-enclosed Bay with mariculture (Dongshan Bay, Southern China) |
| title_fullStr | Disentangling the abundance and structure of Vibrio communities in a semi-enclosed Bay with mariculture (Dongshan Bay, Southern China) |
| title_full_unstemmed | Disentangling the abundance and structure of Vibrio communities in a semi-enclosed Bay with mariculture (Dongshan Bay, Southern China) |
| title_short | Disentangling the abundance and structure of Vibrio communities in a semi-enclosed Bay with mariculture (Dongshan Bay, Southern China) |
| title_sort | disentangling the abundance and structure of vibrio communities in a semi enclosed bay with mariculture dongshan bay southern china |
| topic | 16S rRNA Cfu Vibrio diversity Vibrio abundance Mariculture Dongshan bay |
| url | http://www.sciencedirect.com/science/article/pii/S2001037021003305 |
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