Performance of Spectrophotometric and Fluorometric DNA Quantification Methods

Performance of Spectrophotometric and Fluorometric DNA Quantification Methods

Accurate DNA quantification is a highly important method within molecular biology. Methods widely used to quantify DNA are UV spectrometry and fluorometry. In this research, seven different DNA samples and one blank (MilliQ ultrapure water) were quantified by three analysts using one spectrophotomet...

Full description

Bibliographic Details
Main Authors:	Brigitte Bruijns, Tina Hoekema, Lisa Oomens, Roald Tiggelaar, Han Gardeniers
Format:	Article
Language:	English
Published:	MDPI AG 2022-09-01
Series:	Analytica
Subjects:	DNA quantification absorbance fluorescence
Online Access:	https://www.mdpi.com/2673-4532/3/3/25

Similar Items

A Microfluidic Approach for Biosensing DNA within Forensics
by: Brigitte Bruijns, et al.
Published: (2020-10-01)

Dataset of the absorption, emission and excitation spectra and fluorescence intensity graphs of fluorescent cyanine dyes for the quantification of low amounts of dsDNA
by: Brigitte Bruijns, et al.
Published: (2017-02-01)

A Systematic Review on Commercially Available Integrated Systems for Forensic DNA Analysis
by: Brigitte Bruijns, et al.
Published: (2023-01-01)

A manual of fluorometric and spectrophotometric experiments/
by: Gillespie, Allesia M
Published: (1985)

Quantification of Hypochlorite in Water Using the Nutritional Food Additive Pyridoxamine
by: Kamilla M. S. Kaarsholm, et al.
Published: (2021-12-01)

Cyclic Olefin Copolymer Microfluidic Devices for Forensic Applications
by: Brigitte Bruijns, et al.
Published: (2019-07-01)

Automated quantification of fluorescence and morphological changes in pretreated wood cells by fluorescence macroscopy
by: Edwige Audibert, et al.
Published: (2023-02-01)

FDA authorized molecular point-of-care SARS-CoV-2 tests: A critical review on principles, systems and clinical performances
by: Brigitte Bruijns, et al.
Published: (2022-09-01)

High Performance Liquid Chromatography (HPLC) with Fluorescence Detection for Quantification of Steroids in Clinical, Pharmaceutical, and Environmental Samples: A Review
by: Fatima Hameedat, et al.
Published: (2022-03-01)

Validation of Rapid Enzymatic Quantification of Acetic Acid in Vinegar on Automated Spectrophotometric System
by: Irene Dini, et al.
Published: (2020-06-01)

DNA Dyes—Highly Sensitive Reporters of Cell Quantification: Comparison with Other Cell Quantification Methods
by: Anna Ligasová, et al.
Published: (2021-09-01)

Comparation of Spectrophotometric and Fluorometric Measurements of Chlorophyll-a
by: Carlos Rivera R., et al.
Published: (2005-07-01)

Development of a fluorescent quantification strip assay for the detection of lead
by: Shaozhen Li, et al.
Published: (2020-01-01)

Effect of the Matrix and Target on the Accurate Quantification of Genomic and Plasmid DNA by Digital Polymerase Chain Reaction
by: Nengwu Si, et al.
Published: (2023-01-01)

A time-series approach to random number generation: Using recurrence quantification analysis to capture executive behavior
by: Wouter eOomens, et al.
Published: (2015-06-01)

Intrinsic Fluorometric Reporters of Pteridine Reductase 1, a Target for Antiparasitic Agents
by: Stefania Ferrari, et al.
Published: (2022-05-01)

Automatic Quantification of Cardiomyocyte Dimensions and Connexin 43 Lateralization in Fluorescence Images
by: Antoni Oliver-Gelabert, et al.
Published: (2020-09-01)

Promising Perspectives for Detection, Identification, and Quantification of Plant Pathogenic Fungi and Oomycetes through Targeting Mitochondrial DNA
by: Tomasz Kulik, et al.
Published: (2020-04-01)

Comparison of UV spectrometry and fluorometry-based methods for quantification of cell-free DNA in red cell components
by: Dheeraj Khetan, et al.
Published: (2019-01-01)

An inexpensive, customizable microscopy system for the automated quantification and characterization of multiple adherent cell types
by: Vishwaratn Asthana, et al.
Published: (2018-06-01)

Quantification of sulfur distribution on rubber surfaces by means of μ-X-ray fluorescence analysis
by: S. Frosch, et al.
Published: (2023-11-01)

Making the Most of Lateral Flow Immunochromatographic Tests: An Efficient Protocol to Recover DNA
by: Sara C. Zapico, et al.
Published: (2024-01-01)

Quantification of knowledge content of a high impact innovation: recombinant DNA
by: Hung Tseng, et al.
Published: (2019-08-01)

Control of Energy Transfer Between Pyrene‐ and Perylene‐Nucleosides by the Sequence of DNA‐Templated Supramolecular Assemblies
by: Sara Müller, et al.
Published: (2020-04-01)

UV-Vis Spectrophotometric Analysis of DNA Retrieval for DNA Storage Applications
by: Aamir Minhas-Khan, et al.
Published: (2021-09-01)

Inconsistent virus DNA quantification results in clinical hepatitis B and the verification by genotyping and sequencing: a case report
by: Chen Hanxiang, et al.
Published: (2020-12-01)

Use of Lateral Flow Assays in Forensics
by: Brigitte Bruijns, et al.
Published: (2023-07-01)

A Dihydropyridine Derivative as a Highly Selective Fluorometric Probe for Quantification of Au<sup>3+</sup> Residue in Gold Nanoparticle Solution
by: Waroton Paisuwan, et al.
Published: (2022-12-01)

Evaluation of a ddPCR Commercial Assay for the Absolute Quantification of the Monkeypox Virus West Africa in Clinical Samples
by: Elena Pomari, et al.
Published: (2023-04-01)

Molecular Imaging and Quantification of Smooth Muscle Cell and Aortic Tissue Calcification In Vitro and Ex Vivo with a Fluorescent Hydroxyapatite-Specific Probe
by: Anna Greco, et al.
Published: (2022-09-01)

Fluorometric Quantification of Total Cell-Free DNA as a Prognostic Biomarker in Non-Small-Cell Lung Cancer Patients Treated with Immune Checkpoint Blockade
by: Javier Oliver, et al.
Published: (2023-06-01)

Extracting the invisible: obtaining high quality DNA is a challenging task in small arthropods
by: Andrea Lienhard, et al.
Published: (2019-04-01)

Fluorometric Detection of Oil Traces in a Sea Water Column
by: Emilia Baszanowska, et al.
Published: (2022-03-01)

Definiteness-indefiniteness category and logical quantification
by: Violetta Koseska-Toszewa
Published: (2015-11-01)

Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification?
by: CRISTIAN GALLARDO-ESCÁRATE, et al.
Published: (2007-01-01)

Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification?
by: CRISTIAN GALLARDO-ESCÁRATE, et al.
Published: (2007-01-01)

Mitochondrial DNA quantification correlates with the developmental potential of human euploid blastocysts but not with that of mosaic blastocysts
by: Wen Luo, et al.
Published: (2023-06-01)

Absolute quantification of DNA damage response proteins
by: Shun Matsuda, et al.
Published: (2023-12-01)

A DNA/RNA hybrid fluorescent probe for high-throughput quantification of the activity of human apurinic/apyrimidinic endonuclease 1 in subcellular extracts
by: Peng Lu, et al.
Published: (2023-09-01)

Quantification of HEV RNA by Droplet Digital PCR
by: Florence Nicot, et al.
Published: (2016-08-01)