Evaluation of diagnostic reliability of micronuclei in potentially malignant disorders of oral cavity
Context: Micronucleus (MN) has been proved to be an important biomarker of genomic damage. MN scoring in oral exfoliated epithelial cells has been used in the past as a biomarker to identify different preneoplastic conditions much earlier than the manifestations of clinical features. However, no att...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-01-01
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| Series: | CHRISMED Journal of Health and Research |
| Subjects: | |
| Online Access: | http://www.cjhr.org/article.asp?issn=2348-3334;year=2014;volume=1;issue=1;spage=15;epage=20;aulast=Grover |
| _version_ | 1818508422797066240 |
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| author | Sonal Grover Mujib BR Ahmed Neethu Telagi Anil B Shivappa K P Nithin |
| author_facet | Sonal Grover Mujib BR Ahmed Neethu Telagi Anil B Shivappa K P Nithin |
| author_sort | Sonal Grover |
| collection | DOAJ |
| description | Context: Micronucleus (MN) has been proved to be an important biomarker of genomic damage. MN scoring in oral exfoliated epithelial cells has been used in the past as a biomarker to identify different preneoplastic conditions much earlier than the manifestations of clinical features. However, no attention has been, until now, given to determine the overall accuracy of MN assay in diagnosing potentially malignant disorders (PMDs). Aim: The present study was being conducted to evaluate the diagnostic accuracy of MN assay in PMDs of oral cavity. Materials and Methods: Cytosmears from 30 controls with healthy mucosa and 45 cases as patients with suspicious lesions, clinically diagnosed as PMDs of the oral cavity were taken and separately stained with Papanicolaou (Pap) stain and haematoxylin and eosin (H and E) stain. MN frequency was evaluated and based on the 50 th percentile of all the MN (%) frequency values obtained for both the groups combined; a cut off value was calculated independently for each stain. Results: The sensitivity and specificity with Pap stain was found to be 84% and 93%, respectively, with diagnostic accuracy of 88%, whereas with H and E stain, sensitivity of 89% and specificity of 100% with diagnostic accuracy of 93% was observed. Conclusion: MN assay in oral exfoliated epithelial cells can be conveniently used as a biomarker for the screening of PMDs of oral cavity. |
| first_indexed | 2024-12-10T22:31:41Z |
| format | Article |
| id | doaj.art-ceb0c3474fb4442d9790f871d9c04084 |
| institution | Directory Open Access Journal |
| issn | 2348-3334 2348-506X |
| language | English |
| last_indexed | 2024-12-10T22:31:41Z |
| publishDate | 2014-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | CHRISMED Journal of Health and Research |
| spelling | doaj.art-ceb0c3474fb4442d9790f871d9c040842022-12-22T01:31:02ZengWolters Kluwer Medknow PublicationsCHRISMED Journal of Health and Research2348-33342348-506X2014-01-0111152010.4103/2348-3334.126776Evaluation of diagnostic reliability of micronuclei in potentially malignant disorders of oral cavitySonal GroverMujib BR AhmedNeethu TelagiAnil B ShivappaK P NithinContext: Micronucleus (MN) has been proved to be an important biomarker of genomic damage. MN scoring in oral exfoliated epithelial cells has been used in the past as a biomarker to identify different preneoplastic conditions much earlier than the manifestations of clinical features. However, no attention has been, until now, given to determine the overall accuracy of MN assay in diagnosing potentially malignant disorders (PMDs). Aim: The present study was being conducted to evaluate the diagnostic accuracy of MN assay in PMDs of oral cavity. Materials and Methods: Cytosmears from 30 controls with healthy mucosa and 45 cases as patients with suspicious lesions, clinically diagnosed as PMDs of the oral cavity were taken and separately stained with Papanicolaou (Pap) stain and haematoxylin and eosin (H and E) stain. MN frequency was evaluated and based on the 50 th percentile of all the MN (%) frequency values obtained for both the groups combined; a cut off value was calculated independently for each stain. Results: The sensitivity and specificity with Pap stain was found to be 84% and 93%, respectively, with diagnostic accuracy of 88%, whereas with H and E stain, sensitivity of 89% and specificity of 100% with diagnostic accuracy of 93% was observed. Conclusion: MN assay in oral exfoliated epithelial cells can be conveniently used as a biomarker for the screening of PMDs of oral cavity.http://www.cjhr.org/article.asp?issn=2348-3334;year=2014;volume=1;issue=1;spage=15;epage=20;aulast=GroverMicronucleuspotentially malignant disorderpapanicolaou stainhaematoxylin and eosin stain |
| spellingShingle | Sonal Grover Mujib BR Ahmed Neethu Telagi Anil B Shivappa K P Nithin Evaluation of diagnostic reliability of micronuclei in potentially malignant disorders of oral cavity CHRISMED Journal of Health and Research Micronucleus potentially malignant disorder papanicolaou stain haematoxylin and eosin stain |
| title | Evaluation of diagnostic reliability of micronuclei in potentially malignant disorders of oral cavity |
| title_full | Evaluation of diagnostic reliability of micronuclei in potentially malignant disorders of oral cavity |
| title_fullStr | Evaluation of diagnostic reliability of micronuclei in potentially malignant disorders of oral cavity |
| title_full_unstemmed | Evaluation of diagnostic reliability of micronuclei in potentially malignant disorders of oral cavity |
| title_short | Evaluation of diagnostic reliability of micronuclei in potentially malignant disorders of oral cavity |
| title_sort | evaluation of diagnostic reliability of micronuclei in potentially malignant disorders of oral cavity |
| topic | Micronucleus potentially malignant disorder papanicolaou stain haematoxylin and eosin stain |
| url | http://www.cjhr.org/article.asp?issn=2348-3334;year=2014;volume=1;issue=1;spage=15;epage=20;aulast=Grover |
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