Optofluidic Sensor Based on Polymer Optical Microresonators for the Specific, Sensitive and Fast Detection of Chemical and Biochemical Species
The accurate, rapid, and specific detection of DNA strands in solution is becoming increasingly important, especially in biomedical applications such as the trace detection of COVID-19 or cancer diagnosis. In this work we present the design, elaboration and characterization of an optofluidic sensor...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-08-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/23/17/7373 |
| _version_ | 1797581872918691840 |
|---|---|
| author | Nolwenn-Amandine Keriel Camille Delezoide David Chauvin Hafsa Korri-Youssoufi Ngoc Diep Lai Isabelle Ledoux-Rak Chi-Thanh Nguyen |
| author_facet | Nolwenn-Amandine Keriel Camille Delezoide David Chauvin Hafsa Korri-Youssoufi Ngoc Diep Lai Isabelle Ledoux-Rak Chi-Thanh Nguyen |
| author_sort | Nolwenn-Amandine Keriel |
| collection | DOAJ |
| description | The accurate, rapid, and specific detection of DNA strands in solution is becoming increasingly important, especially in biomedical applications such as the trace detection of COVID-19 or cancer diagnosis. In this work we present the design, elaboration and characterization of an optofluidic sensor based on a polymer-based microresonator which shows a quick response time, a low detection limit and good sensitivity. The device is composed of a micro-racetrack waveguide vertically coupled to a bus waveguide and embedded within a microfluidic circuit. The spectral response of the microresonator, in air or immersed in deionised water, shows quality factors up to 72,900 and contrasts up to 0.9. The concentration of DNA strands in water is related to the spectral shift of the microresonator transmission function, as measured at the inflection points of resonance peaks in order to optimize the signal-over-noise ratio. After functionalization by a DNA probe strand on the surface of the microresonator, a specific and real time measurement of the complementary DNA strands in the solution is realized. Additionally, we have inferred the dissociation constant value of the binding equilibrium of the two complementary DNA strands and evidenced a sensitivity of 16.0 pm/µM and a detection limit of 121 nM. |
| first_indexed | 2024-03-10T23:13:48Z |
| format | Article |
| id | doaj.art-ceb4568dfa714395b3fa5f8f077b621e |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T23:13:48Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-ceb4568dfa714395b3fa5f8f077b621e2023-11-19T08:49:01ZengMDPI AGSensors1424-82202023-08-012317737310.3390/s23177373Optofluidic Sensor Based on Polymer Optical Microresonators for the Specific, Sensitive and Fast Detection of Chemical and Biochemical SpeciesNolwenn-Amandine Keriel0Camille Delezoide1David Chauvin2Hafsa Korri-Youssoufi3Ngoc Diep Lai4Isabelle Ledoux-Rak5Chi-Thanh Nguyen6Laboratoire Lumière, Matière et Interfaces (LuMIn), Ecole Normale Superieure Paris Saclay, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 9024, CentraleSupelec, Institut d’Alembert, Université Paris Saclay, 4 Avenue des Sciences, 91190 Gif-sur-Yvette, FranceLaboratoire Lumière, Matière et Interfaces (LuMIn), Ecole Normale Superieure Paris Saclay, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 9024, CentraleSupelec, Institut d’Alembert, Université Paris Saclay, 4 Avenue des Sciences, 91190 Gif-sur-Yvette, FranceLaboratoire Lumière, Matière et Interfaces (LuMIn), Ecole Normale Superieure Paris Saclay, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 9024, CentraleSupelec, Institut d’Alembert, Université Paris Saclay, 4 Avenue des Sciences, 91190 Gif-sur-Yvette, FranceInstitut de Chimie Moléculaire et des Matériaux d’Orsay (ICMMO), Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8182, Université Paris Saclay, 17 Avenue des Sciences, 91400 Orsay, FranceLaboratoire Lumière, Matière et Interfaces (LuMIn), Ecole Normale Superieure Paris Saclay, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 9024, CentraleSupelec, Institut d’Alembert, Université Paris Saclay, 4 Avenue des Sciences, 91190 Gif-sur-Yvette, FranceLaboratoire Lumière, Matière et Interfaces (LuMIn), Ecole Normale Superieure Paris Saclay, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 9024, CentraleSupelec, Institut d’Alembert, Université Paris Saclay, 4 Avenue des Sciences, 91190 Gif-sur-Yvette, FranceLaboratoire Lumière, Matière et Interfaces (LuMIn), Ecole Normale Superieure Paris Saclay, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 9024, CentraleSupelec, Institut d’Alembert, Université Paris Saclay, 4 Avenue des Sciences, 91190 Gif-sur-Yvette, FranceThe accurate, rapid, and specific detection of DNA strands in solution is becoming increasingly important, especially in biomedical applications such as the trace detection of COVID-19 or cancer diagnosis. In this work we present the design, elaboration and characterization of an optofluidic sensor based on a polymer-based microresonator which shows a quick response time, a low detection limit and good sensitivity. The device is composed of a micro-racetrack waveguide vertically coupled to a bus waveguide and embedded within a microfluidic circuit. The spectral response of the microresonator, in air or immersed in deionised water, shows quality factors up to 72,900 and contrasts up to 0.9. The concentration of DNA strands in water is related to the spectral shift of the microresonator transmission function, as measured at the inflection points of resonance peaks in order to optimize the signal-over-noise ratio. After functionalization by a DNA probe strand on the surface of the microresonator, a specific and real time measurement of the complementary DNA strands in the solution is realized. Additionally, we have inferred the dissociation constant value of the binding equilibrium of the two complementary DNA strands and evidenced a sensitivity of 16.0 pm/µM and a detection limit of 121 nM.https://www.mdpi.com/1424-8220/23/17/7373microresonatorDNA detectionoptofluidicspolymer-based waveguides |
| spellingShingle | Nolwenn-Amandine Keriel Camille Delezoide David Chauvin Hafsa Korri-Youssoufi Ngoc Diep Lai Isabelle Ledoux-Rak Chi-Thanh Nguyen Optofluidic Sensor Based on Polymer Optical Microresonators for the Specific, Sensitive and Fast Detection of Chemical and Biochemical Species Sensors microresonator DNA detection optofluidics polymer-based waveguides |
| title | Optofluidic Sensor Based on Polymer Optical Microresonators for the Specific, Sensitive and Fast Detection of Chemical and Biochemical Species |
| title_full | Optofluidic Sensor Based on Polymer Optical Microresonators for the Specific, Sensitive and Fast Detection of Chemical and Biochemical Species |
| title_fullStr | Optofluidic Sensor Based on Polymer Optical Microresonators for the Specific, Sensitive and Fast Detection of Chemical and Biochemical Species |
| title_full_unstemmed | Optofluidic Sensor Based on Polymer Optical Microresonators for the Specific, Sensitive and Fast Detection of Chemical and Biochemical Species |
| title_short | Optofluidic Sensor Based on Polymer Optical Microresonators for the Specific, Sensitive and Fast Detection of Chemical and Biochemical Species |
| title_sort | optofluidic sensor based on polymer optical microresonators for the specific sensitive and fast detection of chemical and biochemical species |
| topic | microresonator DNA detection optofluidics polymer-based waveguides |
| url | https://www.mdpi.com/1424-8220/23/17/7373 |
| work_keys_str_mv | AT nolwennamandinekeriel optofluidicsensorbasedonpolymeropticalmicroresonatorsforthespecificsensitiveandfastdetectionofchemicalandbiochemicalspecies AT camilledelezoide optofluidicsensorbasedonpolymeropticalmicroresonatorsforthespecificsensitiveandfastdetectionofchemicalandbiochemicalspecies AT davidchauvin optofluidicsensorbasedonpolymeropticalmicroresonatorsforthespecificsensitiveandfastdetectionofchemicalandbiochemicalspecies AT hafsakorriyoussoufi optofluidicsensorbasedonpolymeropticalmicroresonatorsforthespecificsensitiveandfastdetectionofchemicalandbiochemicalspecies AT ngocdieplai optofluidicsensorbasedonpolymeropticalmicroresonatorsforthespecificsensitiveandfastdetectionofchemicalandbiochemicalspecies AT isabelleledouxrak optofluidicsensorbasedonpolymeropticalmicroresonatorsforthespecificsensitiveandfastdetectionofchemicalandbiochemicalspecies AT chithanhnguyen optofluidicsensorbasedonpolymeropticalmicroresonatorsforthespecificsensitiveandfastdetectionofchemicalandbiochemicalspecies |