mTOR signaling pathway regulates embryonic development and rapid growth of triploid crucian carp
Triploid crucian carp is a new breed of aquaculture with rapid growth characteristics. However, its growth regulation mechanism has not yet been elucidated. In this study, our results show that the expression levels of mTOR signaling pathway-related genes (AKT1, AKT2, AKT3, mTOR, 4E-BP1 and S6K1) ar...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S235251342300399X |
| _version_ | 1797388770501197824 |
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| author | Zhen Huang Liuye Dai Fangyuan Peng Lingwei Tang Xuejing Wang Jiayan Chen Jinhui Liu Wen Fu Liangyue Peng Wenbin Liu Yamei Xiao |
| author_facet | Zhen Huang Liuye Dai Fangyuan Peng Lingwei Tang Xuejing Wang Jiayan Chen Jinhui Liu Wen Fu Liangyue Peng Wenbin Liu Yamei Xiao |
| author_sort | Zhen Huang |
| collection | DOAJ |
| description | Triploid crucian carp is a new breed of aquaculture with rapid growth characteristics. However, its growth regulation mechanism has not yet been elucidated. In this study, our results show that the expression levels of mTOR signaling pathway-related genes (AKT1, AKT2, AKT3, mTOR, 4E-BP1 and S6K1) are higher in the muscle and intestine tissues of triploid crucian carp than those of diploid red crucian carp. Embryos of triploid crucian carp exhibit a faster growth rate comparing to red crucian carp. And in muscle effect stage, the mRNA levels of mTOR signaling pathway related genes in triploid crucian carp are higher than red crucian carp, except for AKT2. Inhibition of mTOR activity with rapamycin leads to slower embryonic development and the eliminated expression of mTOR in the embryos of neurula, muscle effect, and eye pigmentation stages. Conversely, overexpression of the upstream gene AKT2 can promote the development of zebrafish embryos and affect the expression of mTOR signaling pathway. These data highlight the significant role of mTOR signaling pathway in regulating fish embryonic development and rapid growth, and might offer new insights and avenues for exploring fish embryonic development, improving fish growth. |
| first_indexed | 2024-03-08T22:45:37Z |
| format | Article |
| id | doaj.art-ceb53f9f00e14729961ef62d799a6726 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-08T22:45:37Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-ceb53f9f00e14729961ef62d799a67262023-12-17T06:40:00ZengElsevierAquaculture Reports2352-51342023-12-0133101860mTOR signaling pathway regulates embryonic development and rapid growth of triploid crucian carpZhen Huang0Liuye Dai1Fangyuan Peng2Lingwei Tang3Xuejing Wang4Jiayan Chen5Jinhui Liu6Wen Fu7Liangyue Peng8Wenbin Liu9Yamei Xiao10State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha 410081, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Changsha 410081, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha 410081, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Changsha 410081, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha 410081, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Changsha 410081, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha 410081, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Changsha 410081, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha 410081, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Changsha 410081, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha 410081, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Changsha 410081, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha 410081, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Changsha 410081, China; College of Life Sciences, Hunan Normal University, Changsha 410081, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha 410081, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Changsha 410081, China; College of Life Sciences, Hunan Normal University, Changsha 410081, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha 410081, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Changsha 410081, China; College of Life Sciences, Hunan Normal University, Changsha 410081, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha 410081, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Changsha 410081, China; College of Life Sciences, Hunan Normal University, Changsha 410081, China; Corresponding authors at: State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha 410081, China.State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha 410081, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Changsha 410081, China; College of Life Sciences, Hunan Normal University, Changsha 410081, China; Corresponding authors at: State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha 410081, China.Triploid crucian carp is a new breed of aquaculture with rapid growth characteristics. However, its growth regulation mechanism has not yet been elucidated. In this study, our results show that the expression levels of mTOR signaling pathway-related genes (AKT1, AKT2, AKT3, mTOR, 4E-BP1 and S6K1) are higher in the muscle and intestine tissues of triploid crucian carp than those of diploid red crucian carp. Embryos of triploid crucian carp exhibit a faster growth rate comparing to red crucian carp. And in muscle effect stage, the mRNA levels of mTOR signaling pathway related genes in triploid crucian carp are higher than red crucian carp, except for AKT2. Inhibition of mTOR activity with rapamycin leads to slower embryonic development and the eliminated expression of mTOR in the embryos of neurula, muscle effect, and eye pigmentation stages. Conversely, overexpression of the upstream gene AKT2 can promote the development of zebrafish embryos and affect the expression of mTOR signaling pathway. These data highlight the significant role of mTOR signaling pathway in regulating fish embryonic development and rapid growth, and might offer new insights and avenues for exploring fish embryonic development, improving fish growth.http://www.sciencedirect.com/science/article/pii/S235251342300399XTriploid crucian carpmTOR signaling pathwayEmbryonic developmentRapid growth |
| spellingShingle | Zhen Huang Liuye Dai Fangyuan Peng Lingwei Tang Xuejing Wang Jiayan Chen Jinhui Liu Wen Fu Liangyue Peng Wenbin Liu Yamei Xiao mTOR signaling pathway regulates embryonic development and rapid growth of triploid crucian carp Aquaculture Reports Triploid crucian carp mTOR signaling pathway Embryonic development Rapid growth |
| title | mTOR signaling pathway regulates embryonic development and rapid growth of triploid crucian carp |
| title_full | mTOR signaling pathway regulates embryonic development and rapid growth of triploid crucian carp |
| title_fullStr | mTOR signaling pathway regulates embryonic development and rapid growth of triploid crucian carp |
| title_full_unstemmed | mTOR signaling pathway regulates embryonic development and rapid growth of triploid crucian carp |
| title_short | mTOR signaling pathway regulates embryonic development and rapid growth of triploid crucian carp |
| title_sort | mtor signaling pathway regulates embryonic development and rapid growth of triploid crucian carp |
| topic | Triploid crucian carp mTOR signaling pathway Embryonic development Rapid growth |
| url | http://www.sciencedirect.com/science/article/pii/S235251342300399X |
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