Non‐invasive omics analysis delineates molecular changes in water‐only fasting and its sex‐discriminating features in metabolic syndrome patients
Abstract Fasting has been grown in popularity with multiple potential benefits. However, very few studies dynamically monitor physiological and pathological changes during long‐term fasting using noninvasive methods. In the present study, we recruited 37 individuals with metabolic syndrome to engage...
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| Format: | Article |
| Language: | English |
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Wiley
2023-12-01
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| Series: | MedComm |
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| Online Access: | https://doi.org/10.1002/mco2.393 |
| _version_ | 1797373570566848512 |
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| author | Yanyu Jiang Zhimei Tang Xiaogang Zhu Biying Xiao Hechuan Tian Xingxing Lei Huan Peng Jun Qin Yanmei Zhang Robert M. Hoffman Xiaorong Hu Qiu Chen Guang Ji Lijun Jia |
| author_facet | Yanyu Jiang Zhimei Tang Xiaogang Zhu Biying Xiao Hechuan Tian Xingxing Lei Huan Peng Jun Qin Yanmei Zhang Robert M. Hoffman Xiaorong Hu Qiu Chen Guang Ji Lijun Jia |
| author_sort | Yanyu Jiang |
| collection | DOAJ |
| description | Abstract Fasting has been grown in popularity with multiple potential benefits. However, very few studies dynamically monitor physiological and pathological changes during long‐term fasting using noninvasive methods. In the present study, we recruited 37 individuals with metabolic syndrome to engage in a 5‐day water‐only fasting regimen, and simultaneously captured the molecular alterations through urinary proteomics and metabolomics. Our findings reveal that water‐only fasting significantly mitigated metabolic syndrome‐related risk markers, such as body weight, body mass index, abdominal circumference, blood pressure, and fasting blood glucose levels in metabolic syndrome patients. Indicators of liver and renal function remained within the normal range, with the exception of uric acid. Notably, inflammatory response was inhibited during the water‐only fasting period, as evidenced by a decrease in the human monocyte differentiation antigen CD14. Intriguingly, glycolysis, tricarboxylic acid cycle, and oxidative phosphorylation underwent a sex‐dependent reprogramming throughout the fasting period, whereby males exhibited a greater upregulation of carbohydrate metabolism‐related enzymes than females. This disparity may be attributed to evolutionary pressures. Collectively, our study sheds light on the beneficial physiological effects and novel dynamic molecular features associated with fasting in individuals with metabolic syndrome using noninvasive methods. |
| first_indexed | 2024-03-08T18:52:24Z |
| format | Article |
| id | doaj.art-cebe02e949d24cd8a1ee34245b4c5cca |
| institution | Directory Open Access Journal |
| issn | 2688-2663 |
| language | English |
| last_indexed | 2024-03-08T18:52:24Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | MedComm |
| spelling | doaj.art-cebe02e949d24cd8a1ee34245b4c5cca2023-12-28T16:52:34ZengWileyMedComm2688-26632023-12-0146n/an/a10.1002/mco2.393Non‐invasive omics analysis delineates molecular changes in water‐only fasting and its sex‐discriminating features in metabolic syndrome patientsYanyu Jiang0Zhimei Tang1Xiaogang Zhu2Biying Xiao3Hechuan Tian4Xingxing Lei5Huan Peng6Jun Qin7Yanmei Zhang8Robert M. Hoffman9Xiaorong Hu10Qiu Chen11Guang Ji12Lijun Jia13Cancer Institute Longhua Hospital Shanghai University of Traditional Chinese Medicine Shanghai ChinaHospital of Chengdu University of Traditional Chinese Medicine Sichuan ChinaDujiangyan Diabetes Hospital Sichuan ChinaCancer Institute Longhua Hospital Shanghai University of Traditional Chinese Medicine Shanghai ChinaCancer Institute Longhua Hospital Shanghai University of Traditional Chinese Medicine Shanghai ChinaHospital of Chengdu University of Traditional Chinese Medicine Sichuan ChinaHospital of Chengdu University of Traditional Chinese Medicine Sichuan ChinaState Key Laboratory of Proteomics Beijing Proteome Research Center National Center for Protein Sciences (Beijing) Beijing Institute of Lifeomics Beijing ChinaDepartment of Laboratory Medicine Huadong Hospital Fudan University Shanghai ChinaDepartment of Surgery University of California San Diego San Diego California USADujiangyan Diabetes Hospital Sichuan ChinaHospital of Chengdu University of Traditional Chinese Medicine Sichuan ChinaInstitute of Digestive Diseases Longhua Hospital Shanghai University of Traditional Chinese Medicine Shanghai ChinaCancer Institute Longhua Hospital Shanghai University of Traditional Chinese Medicine Shanghai ChinaAbstract Fasting has been grown in popularity with multiple potential benefits. However, very few studies dynamically monitor physiological and pathological changes during long‐term fasting using noninvasive methods. In the present study, we recruited 37 individuals with metabolic syndrome to engage in a 5‐day water‐only fasting regimen, and simultaneously captured the molecular alterations through urinary proteomics and metabolomics. Our findings reveal that water‐only fasting significantly mitigated metabolic syndrome‐related risk markers, such as body weight, body mass index, abdominal circumference, blood pressure, and fasting blood glucose levels in metabolic syndrome patients. Indicators of liver and renal function remained within the normal range, with the exception of uric acid. Notably, inflammatory response was inhibited during the water‐only fasting period, as evidenced by a decrease in the human monocyte differentiation antigen CD14. Intriguingly, glycolysis, tricarboxylic acid cycle, and oxidative phosphorylation underwent a sex‐dependent reprogramming throughout the fasting period, whereby males exhibited a greater upregulation of carbohydrate metabolism‐related enzymes than females. This disparity may be attributed to evolutionary pressures. Collectively, our study sheds light on the beneficial physiological effects and novel dynamic molecular features associated with fasting in individuals with metabolic syndrome using noninvasive methods.https://doi.org/10.1002/mco2.393CD14integrative omics analysesmetabolic profilingmetabolic syndromesex‐discriminating molecular featurewater‐only fasting |
| spellingShingle | Yanyu Jiang Zhimei Tang Xiaogang Zhu Biying Xiao Hechuan Tian Xingxing Lei Huan Peng Jun Qin Yanmei Zhang Robert M. Hoffman Xiaorong Hu Qiu Chen Guang Ji Lijun Jia Non‐invasive omics analysis delineates molecular changes in water‐only fasting and its sex‐discriminating features in metabolic syndrome patients MedComm CD14 integrative omics analyses metabolic profiling metabolic syndrome sex‐discriminating molecular feature water‐only fasting |
| title | Non‐invasive omics analysis delineates molecular changes in water‐only fasting and its sex‐discriminating features in metabolic syndrome patients |
| title_full | Non‐invasive omics analysis delineates molecular changes in water‐only fasting and its sex‐discriminating features in metabolic syndrome patients |
| title_fullStr | Non‐invasive omics analysis delineates molecular changes in water‐only fasting and its sex‐discriminating features in metabolic syndrome patients |
| title_full_unstemmed | Non‐invasive omics analysis delineates molecular changes in water‐only fasting and its sex‐discriminating features in metabolic syndrome patients |
| title_short | Non‐invasive omics analysis delineates molecular changes in water‐only fasting and its sex‐discriminating features in metabolic syndrome patients |
| title_sort | non invasive omics analysis delineates molecular changes in water only fasting and its sex discriminating features in metabolic syndrome patients |
| topic | CD14 integrative omics analyses metabolic profiling metabolic syndrome sex‐discriminating molecular feature water‐only fasting |
| url | https://doi.org/10.1002/mco2.393 |
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