The role of IL17 and IL17RA polymorphisms in lethal pandemic acute viral pneumonia (Influenza A virus H1N1 subtype)
Abstract Background The cytokines play an essential role in acute inflammatory processes, and the IL-17 may be responsible for ambiguous aspects, and the correlation with genetic polymorphisms could improve the search for this critical biomarker. Thus, this study aimed to evaluate the IL-17A and IL-...
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | Surgical and Experimental Pathology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s42047-023-00126-0 |
| _version_ | 1828035405452148736 |
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| author | Vanessa Yumie Salomão Watanabe Liberalesso Marina Luise Viola Azevedo Mineia Alessandra Scaranello Malaquias Caroline Busatta Vaz de Paula Seigo Nagashima Daiane Gavlik de Souza Plínio Cézar Neto Kauana Oliveira Gouveia Larissa Cristina Biscaro Ana Luisa Garcia Giamberardino Gabrielle Tasso Gonçalves Thais Teles Soares Kondo Sonia Maria Raboni Isabelle Weiss Cleber Machado-Souza Lucia de Noronha |
| author_facet | Vanessa Yumie Salomão Watanabe Liberalesso Marina Luise Viola Azevedo Mineia Alessandra Scaranello Malaquias Caroline Busatta Vaz de Paula Seigo Nagashima Daiane Gavlik de Souza Plínio Cézar Neto Kauana Oliveira Gouveia Larissa Cristina Biscaro Ana Luisa Garcia Giamberardino Gabrielle Tasso Gonçalves Thais Teles Soares Kondo Sonia Maria Raboni Isabelle Weiss Cleber Machado-Souza Lucia de Noronha |
| author_sort | Vanessa Yumie Salomão Watanabe Liberalesso |
| collection | DOAJ |
| description | Abstract Background The cytokines play an essential role in acute inflammatory processes, and the IL-17 may be responsible for ambiguous aspects, and the correlation with genetic polymorphisms could improve the search for this critical biomarker. Thus, this study aimed to evaluate the IL-17A and IL-17RA tissue expression and the polymorphisms that codified these proteins in a population that died of pandemic Influenza A virus H1N1 subtype compared to a non-pandemic Influenza virus population. Methods Necropsy lung samples immunohistochemistry was performed to assess the presence of IL-17A and IL-17RA in the pulmonary tissue. Eight single nucleotide polymorphisms were genotyped using TaqMan® technology. Results The Influenza A H1N1 pandemic group had higher tissue expression of IL-17A, higher neutrophil recruitment and shorter survival time between admission and death. Three single nucleotide polymorphisms conferred risk for pandemic influenza A H1N1, the AA genotype of rs3819025 G/A, the CC genotype of rs2241044 A/C, and the TT genotype of rs 2,241,043 C/T. Conclusions One IL17A polymorphism (rs381905) and two IL17RA polymorphisms (rs2241044 and rs2241043) represented biomarkers of worse prognosis in the population infected with pandemic influenza A H1N1. The greater tissue expression of IL-17A shows a Th17 polarization and highlights the aggressiveness of the pandemic influenza virus with its duality in the protection and pathogenesis of the pulmonary infectious process. |
| first_indexed | 2024-04-10T15:45:44Z |
| format | Article |
| id | doaj.art-cebe2c514ce34cac97ccaea4ce034e5c |
| institution | Directory Open Access Journal |
| issn | 2520-8454 |
| language | English |
| last_indexed | 2024-04-10T15:45:44Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Surgical and Experimental Pathology |
| spelling | doaj.art-cebe2c514ce34cac97ccaea4ce034e5c2023-02-12T12:08:16ZengBMCSurgical and Experimental Pathology2520-84542023-02-016111110.1186/s42047-023-00126-0The role of IL17 and IL17RA polymorphisms in lethal pandemic acute viral pneumonia (Influenza A virus H1N1 subtype)Vanessa Yumie Salomão Watanabe Liberalesso0Marina Luise Viola Azevedo1Mineia Alessandra Scaranello Malaquias2Caroline Busatta Vaz de Paula3Seigo Nagashima4Daiane Gavlik de Souza5Plínio Cézar Neto6Kauana Oliveira Gouveia7Larissa Cristina Biscaro8Ana Luisa Garcia Giamberardino9Gabrielle Tasso Gonçalves10Thais Teles Soares Kondo11Sonia Maria Raboni12Isabelle Weiss13Cleber Machado-Souza14Lucia de Noronha15Postgraduation Program in Health Sciences of School of Medicine, Pontifícia Universidade Católica Do ParanáPostgraduation Program in Health Sciences of School of Medicine, Pontifícia Universidade Católica Do ParanáPostgraduation Program in Health Sciences of School of Medicine, Pontifícia Universidade Católica Do ParanáPostgraduation Program in Health Sciences of School of Medicine, Pontifícia Universidade Católica Do ParanáPostgraduation Program in Health Sciences of School of Medicine, Pontifícia Universidade Católica Do ParanáPostgraduation Program in Health Sciences of School of Medicine, Pontifícia Universidade Católica Do ParanáPostgraduation Program in Health Sciences of School of Medicine, Pontifícia Universidade Católica Do ParanáPostgraduation Program in Health Sciences of School of Medicine, Pontifícia Universidade Católica Do ParanáPostgraduation Program in Health Sciences of School of Medicine, Pontifícia Universidade Católica Do ParanáPostgraduation Program in Health Sciences of School of Medicine, Pontifícia Universidade Católica Do ParanáPostgraduation Program in Health Sciences of School of Medicine, Pontifícia Universidade Católica Do ParanáPostgraduation Program in Health Sciences of School of Medicine, Pontifícia Universidade Católica Do ParanáLaboratory of Virology, Hospital de Clínicas, Universidade Federal Do ParanáPostgraduation Program in Biotechnology Applied in Health of Children and Adolescent, Faculdades Pequeno PríncipePostgraduation Program in Biotechnology Applied in Health of Children and Adolescent, Faculdades Pequeno PríncipePostgraduation Program in Health Sciences of School of Medicine, Pontifícia Universidade Católica Do ParanáAbstract Background The cytokines play an essential role in acute inflammatory processes, and the IL-17 may be responsible for ambiguous aspects, and the correlation with genetic polymorphisms could improve the search for this critical biomarker. Thus, this study aimed to evaluate the IL-17A and IL-17RA tissue expression and the polymorphisms that codified these proteins in a population that died of pandemic Influenza A virus H1N1 subtype compared to a non-pandemic Influenza virus population. Methods Necropsy lung samples immunohistochemistry was performed to assess the presence of IL-17A and IL-17RA in the pulmonary tissue. Eight single nucleotide polymorphisms were genotyped using TaqMan® technology. Results The Influenza A H1N1 pandemic group had higher tissue expression of IL-17A, higher neutrophil recruitment and shorter survival time between admission and death. Three single nucleotide polymorphisms conferred risk for pandemic influenza A H1N1, the AA genotype of rs3819025 G/A, the CC genotype of rs2241044 A/C, and the TT genotype of rs 2,241,043 C/T. Conclusions One IL17A polymorphism (rs381905) and two IL17RA polymorphisms (rs2241044 and rs2241043) represented biomarkers of worse prognosis in the population infected with pandemic influenza A H1N1. The greater tissue expression of IL-17A shows a Th17 polarization and highlights the aggressiveness of the pandemic influenza virus with its duality in the protection and pathogenesis of the pulmonary infectious process.https://doi.org/10.1186/s42047-023-00126-0Influenza A virus H1N1 subtypeInfluenzaImmunohistochemistryPolymorphismIL17AIL17RA |
| spellingShingle | Vanessa Yumie Salomão Watanabe Liberalesso Marina Luise Viola Azevedo Mineia Alessandra Scaranello Malaquias Caroline Busatta Vaz de Paula Seigo Nagashima Daiane Gavlik de Souza Plínio Cézar Neto Kauana Oliveira Gouveia Larissa Cristina Biscaro Ana Luisa Garcia Giamberardino Gabrielle Tasso Gonçalves Thais Teles Soares Kondo Sonia Maria Raboni Isabelle Weiss Cleber Machado-Souza Lucia de Noronha The role of IL17 and IL17RA polymorphisms in lethal pandemic acute viral pneumonia (Influenza A virus H1N1 subtype) Surgical and Experimental Pathology Influenza A virus H1N1 subtype Influenza Immunohistochemistry Polymorphism IL17A IL17RA |
| title | The role of IL17 and IL17RA polymorphisms in lethal pandemic acute viral pneumonia (Influenza A virus H1N1 subtype) |
| title_full | The role of IL17 and IL17RA polymorphisms in lethal pandemic acute viral pneumonia (Influenza A virus H1N1 subtype) |
| title_fullStr | The role of IL17 and IL17RA polymorphisms in lethal pandemic acute viral pneumonia (Influenza A virus H1N1 subtype) |
| title_full_unstemmed | The role of IL17 and IL17RA polymorphisms in lethal pandemic acute viral pneumonia (Influenza A virus H1N1 subtype) |
| title_short | The role of IL17 and IL17RA polymorphisms in lethal pandemic acute viral pneumonia (Influenza A virus H1N1 subtype) |
| title_sort | role of il17 and il17ra polymorphisms in lethal pandemic acute viral pneumonia influenza a virus h1n1 subtype |
| topic | Influenza A virus H1N1 subtype Influenza Immunohistochemistry Polymorphism IL17A IL17RA |
| url | https://doi.org/10.1186/s42047-023-00126-0 |
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