Development and Validation of an Allele-Specific Marker for Resistance to Bacterial Halo Blight in <i>Coffea arabica</i>
Bacterial halo blight (BHB) is a bacterial disease, caused by <i>Pseudomonas syringae</i> pv. <i>garcae</i>, which has been gaining prominence in the main coffee-producing regions. Chemical control of this disease increases production costs and is environmentally undesirable....
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/12/3178 |
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| author | Caroline Ariyoshi Gustavo Hiroshi Sera Lucas Mateus Rivero Rodrigues Filipe Gimenez Carvalho Luciana Harumi Shigueoka Ana Ester Socatelli Mendonça Carlos Theodoro Motta Pereira Suzete Aparecida Lanza Destéfano Luiz Filipe Protasio Pereira |
| author_facet | Caroline Ariyoshi Gustavo Hiroshi Sera Lucas Mateus Rivero Rodrigues Filipe Gimenez Carvalho Luciana Harumi Shigueoka Ana Ester Socatelli Mendonça Carlos Theodoro Motta Pereira Suzete Aparecida Lanza Destéfano Luiz Filipe Protasio Pereira |
| author_sort | Caroline Ariyoshi |
| collection | DOAJ |
| description | Bacterial halo blight (BHB) is a bacterial disease, caused by <i>Pseudomonas syringae</i> pv. <i>garcae</i>, which has been gaining prominence in the main coffee-producing regions. Chemical control of this disease increases production costs and is environmentally undesirable. In this scenario, the development of new cultivars resistant to BHB is the most economical and sustainable alternative. Marker-Assisted Selection (MAS) is an appropriate strategy to assist breeding programs for resistant genotype selection. In a previous Genome-Wide Association Study (GWAS) for <i>C. arabica</i> and <i>P. syringae</i> pv. <i>garcae</i> interaction, we identified a locus, probably linked to qualitative resistance to the pathogen. In this work, we developed and validated a pair of Allele-Specific-Polymerase Chain Reaction (AS-PCR) primers for this locus in <i>C. arabica</i> breeding populations. This pair of AS-PCR primers, called Psg_QL1, was tested both in a backcross (BC) (<i>n</i> = 38) and in an F2 population (<i>n</i> = 138) segregating for resistance to BHB. The linkage between the Psg_QL1 marker and qualitative resistance showed an accuracy of 93.75%. Our results demonstrated that the Psg_QL1 marker can be applied in MAS in a robust, simple, fast, and low-cost way. |
| first_indexed | 2024-03-09T17:25:19Z |
| format | Article |
| id | doaj.art-cec2e5261d3c4bc288a53fc240c68ca4 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T17:25:19Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-cec2e5261d3c4bc288a53fc240c68ca42023-11-24T12:47:46ZengMDPI AGAgronomy2073-43952022-12-011212317810.3390/agronomy12123178Development and Validation of an Allele-Specific Marker for Resistance to Bacterial Halo Blight in <i>Coffea arabica</i>Caroline Ariyoshi0Gustavo Hiroshi Sera1Lucas Mateus Rivero Rodrigues2Filipe Gimenez Carvalho3Luciana Harumi Shigueoka4Ana Ester Socatelli Mendonça5Carlos Theodoro Motta Pereira6Suzete Aparecida Lanza Destéfano7Luiz Filipe Protasio Pereira8Programa de Pós-graduação em Genética e Biologia Molecular, Departamento de Ciências Biológicas, Universidade Estadual de Londrina, Londrina 86057970, BrazilÁrea de Melhoramento Genético e Propagação de Plantas, Instituto de Desenvolvimento Rural do Paraná, Londrina 86047902, BrazilCentro de Café Alcides Carvalho, Instituto Agronômico, Campinas 13001970, BrazilÁrea de Melhoramento Genético e Propagação de Plantas, Instituto de Desenvolvimento Rural do Paraná, Londrina 86047902, BrazilÁrea de Melhoramento Genético e Propagação de Plantas, Instituto de Desenvolvimento Rural do Paraná, Londrina 86047902, BrazilÁrea de Melhoramento Genético e Propagação de Plantas, Instituto de Desenvolvimento Rural do Paraná, Londrina 86047902, BrazilÁrea de Melhoramento Genético e Propagação de Plantas, Instituto de Desenvolvimento Rural do Paraná, Londrina 86047902, BrazilInstituto Biológico, Campinas 13012970, BrazilPrograma de Pós-graduação em Genética e Biologia Molecular, Departamento de Ciências Biológicas, Universidade Estadual de Londrina, Londrina 86057970, BrazilBacterial halo blight (BHB) is a bacterial disease, caused by <i>Pseudomonas syringae</i> pv. <i>garcae</i>, which has been gaining prominence in the main coffee-producing regions. Chemical control of this disease increases production costs and is environmentally undesirable. In this scenario, the development of new cultivars resistant to BHB is the most economical and sustainable alternative. Marker-Assisted Selection (MAS) is an appropriate strategy to assist breeding programs for resistant genotype selection. In a previous Genome-Wide Association Study (GWAS) for <i>C. arabica</i> and <i>P. syringae</i> pv. <i>garcae</i> interaction, we identified a locus, probably linked to qualitative resistance to the pathogen. In this work, we developed and validated a pair of Allele-Specific-Polymerase Chain Reaction (AS-PCR) primers for this locus in <i>C. arabica</i> breeding populations. This pair of AS-PCR primers, called Psg_QL1, was tested both in a backcross (BC) (<i>n</i> = 38) and in an F2 population (<i>n</i> = 138) segregating for resistance to BHB. The linkage between the Psg_QL1 marker and qualitative resistance showed an accuracy of 93.75%. Our results demonstrated that the Psg_QL1 marker can be applied in MAS in a robust, simple, fast, and low-cost way.https://www.mdpi.com/2073-4395/12/12/3178AS-PCR primerscoffeeMarker Assisted Selectioncoffee breeding |
| spellingShingle | Caroline Ariyoshi Gustavo Hiroshi Sera Lucas Mateus Rivero Rodrigues Filipe Gimenez Carvalho Luciana Harumi Shigueoka Ana Ester Socatelli Mendonça Carlos Theodoro Motta Pereira Suzete Aparecida Lanza Destéfano Luiz Filipe Protasio Pereira Development and Validation of an Allele-Specific Marker for Resistance to Bacterial Halo Blight in <i>Coffea arabica</i> Agronomy AS-PCR primers coffee Marker Assisted Selection coffee breeding |
| title | Development and Validation of an Allele-Specific Marker for Resistance to Bacterial Halo Blight in <i>Coffea arabica</i> |
| title_full | Development and Validation of an Allele-Specific Marker for Resistance to Bacterial Halo Blight in <i>Coffea arabica</i> |
| title_fullStr | Development and Validation of an Allele-Specific Marker for Resistance to Bacterial Halo Blight in <i>Coffea arabica</i> |
| title_full_unstemmed | Development and Validation of an Allele-Specific Marker for Resistance to Bacterial Halo Blight in <i>Coffea arabica</i> |
| title_short | Development and Validation of an Allele-Specific Marker for Resistance to Bacterial Halo Blight in <i>Coffea arabica</i> |
| title_sort | development and validation of an allele specific marker for resistance to bacterial halo blight in i coffea arabica i |
| topic | AS-PCR primers coffee Marker Assisted Selection coffee breeding |
| url | https://www.mdpi.com/2073-4395/12/12/3178 |
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