The Role of Hydrochar in Promoting Methane Production from Anaerobic Digestion with Different Inocula
Carbon materials, including hydrochar, can promote methane production from anaerobic digestion, and many previous studies have investigated the mechanism by analyzing changes in the microbial community. Based on the fact that the major functional microorganisms have varied in different studies, in o...
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MDPI AG
2023-04-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/5/433 |
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| author | Jieyi Sun Shicheng Zhang Gang Luo |
| author_facet | Jieyi Sun Shicheng Zhang Gang Luo |
| author_sort | Jieyi Sun |
| collection | DOAJ |
| description | Carbon materials, including hydrochar, can promote methane production from anaerobic digestion, and many previous studies have investigated the mechanism by analyzing changes in the microbial community. Based on the fact that the major functional microorganisms have varied in different studies, in order to investigate the effect of inocula on the final microbial composition and to compare the promotion effects of hydrochar on different microorganisms, this study introduced inocula from three distinct sources to anaerobic sequential batch reactors and analyzed the microbial community using 16S rRNA gene sequencing. Hydrochar showed significant promotion effects for all three of the inocula, by increasing microbial activity at high acid concentrations and/or by reducing butyrate accumulation. The dominant microorganisms in all reactors were <i>Clostridium</i> and <i>Methanosarcina</i>, and hydrochar increased the proportion of acetoclastic methanogens. The bacteria which were promoted by hydrochar (e.g., <i>Aminicenantales</i>, <i>Anaerolineaceae</i> and <i>SBR1031</i> in anaerobic granular sludge and sludge digestate, as well as <i>Lachnospiraceae</i> in food waste digestate) only accounted for around 10%. The bacteria found to be involved in DIET in earlier studies were lacking in this study. It was speculated that hydrochar acted as an electron intermediate and supported microbial aggregation, while the possibility that hydrochar promoted DIET cannot be ruled out. |
| first_indexed | 2024-03-11T03:46:05Z |
| format | Article |
| id | doaj.art-cecc1dd0be3d4109a05a13e034148fbb |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T03:46:05Z |
| publishDate | 2023-04-01 |
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| spelling | doaj.art-cecc1dd0be3d4109a05a13e034148fbb2023-11-18T01:17:43ZengMDPI AGFermentation2311-56372023-04-019543310.3390/fermentation9050433The Role of Hydrochar in Promoting Methane Production from Anaerobic Digestion with Different InoculaJieyi Sun0Shicheng Zhang1Gang Luo2Department of Environment Science and Engineering, Fudan University, Shanghai 200433, ChinaDepartment of Environment Science and Engineering, Fudan University, Shanghai 200433, ChinaDepartment of Environment Science and Engineering, Fudan University, Shanghai 200433, ChinaCarbon materials, including hydrochar, can promote methane production from anaerobic digestion, and many previous studies have investigated the mechanism by analyzing changes in the microbial community. Based on the fact that the major functional microorganisms have varied in different studies, in order to investigate the effect of inocula on the final microbial composition and to compare the promotion effects of hydrochar on different microorganisms, this study introduced inocula from three distinct sources to anaerobic sequential batch reactors and analyzed the microbial community using 16S rRNA gene sequencing. Hydrochar showed significant promotion effects for all three of the inocula, by increasing microbial activity at high acid concentrations and/or by reducing butyrate accumulation. The dominant microorganisms in all reactors were <i>Clostridium</i> and <i>Methanosarcina</i>, and hydrochar increased the proportion of acetoclastic methanogens. The bacteria which were promoted by hydrochar (e.g., <i>Aminicenantales</i>, <i>Anaerolineaceae</i> and <i>SBR1031</i> in anaerobic granular sludge and sludge digestate, as well as <i>Lachnospiraceae</i> in food waste digestate) only accounted for around 10%. The bacteria found to be involved in DIET in earlier studies were lacking in this study. It was speculated that hydrochar acted as an electron intermediate and supported microbial aggregation, while the possibility that hydrochar promoted DIET cannot be ruled out.https://www.mdpi.com/2311-5637/9/5/433anaerobic digestionhydrocharmicrobial analysis |
| spellingShingle | Jieyi Sun Shicheng Zhang Gang Luo The Role of Hydrochar in Promoting Methane Production from Anaerobic Digestion with Different Inocula Fermentation anaerobic digestion hydrochar microbial analysis |
| title | The Role of Hydrochar in Promoting Methane Production from Anaerobic Digestion with Different Inocula |
| title_full | The Role of Hydrochar in Promoting Methane Production from Anaerobic Digestion with Different Inocula |
| title_fullStr | The Role of Hydrochar in Promoting Methane Production from Anaerobic Digestion with Different Inocula |
| title_full_unstemmed | The Role of Hydrochar in Promoting Methane Production from Anaerobic Digestion with Different Inocula |
| title_short | The Role of Hydrochar in Promoting Methane Production from Anaerobic Digestion with Different Inocula |
| title_sort | role of hydrochar in promoting methane production from anaerobic digestion with different inocula |
| topic | anaerobic digestion hydrochar microbial analysis |
| url | https://www.mdpi.com/2311-5637/9/5/433 |
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