Hawthorn Procyanidins Regulate Lipid Metabolism through the AMPK/SREBP-1c Signaling Pathway
Objective: To investigate the effect of hawthorn procyanidins (HPC) on lipid metabolism and its mechanism by regulating the AMP-activated protein kinase/sterol regulatory element binding protein-1c (AMPK/SREBP-1c) signaling pathway. Methods: Sixty rats were randomly divided into six groups (n = 10)...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2023-08-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-15-015.pdf |
| _version_ | 1797685195743166464 |
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| author | MI Wei, YU Min, LI Ning, SHI Tala, CHEN Caiyun |
| author_facet | MI Wei, YU Min, LI Ning, SHI Tala, CHEN Caiyun |
| author_sort | MI Wei, YU Min, LI Ning, SHI Tala, CHEN Caiyun |
| collection | DOAJ |
| description | Objective: To investigate the effect of hawthorn procyanidins (HPC) on lipid metabolism and its mechanism by regulating the AMP-activated protein kinase/sterol regulatory element binding protein-1c (AMPK/SREBP-1c) signaling pathway. Methods: Sixty rats were randomly divided into six groups (n = 10) blank control (BC), hyperlipidemia model (HM), low-, medium-, and high-dose HPC (HPC-LD, HPC-MD, and HPC-HD) and fenofibrate positive control (FP). The contents of triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) in the serum and liver homogenate were measured by commercial kits. Hematoxylin eosin (HE) staining was used to observe hepatic histopathological changes. The mRNA expression levels of AMPK, SREBP-1c, glycerol-3-phosphate acyltransferase-1 (GPAT1), acetyl CoA carboxylase (ACC), fatty acid synthase (FAS), carnitine palmitoyltransterase-1 (CPT1) and peroxisome proliferator activated receptor γ coactivator-1α (PGC-1α) in the liver were measured by quantitative real-time polymerase chain reaction (PCR). Western blotting was used to detect the protein expression of p-AMPK, SREBP-1c, GPAT1, ACC, FAS, CPT1 and PGC-1α in the liver. Results: After seven weeks of feeding on a high-fat diet, body mass, liver mass and liver index significantly increased compared with the control group fed on a basal diet (P < 0.05 or < 0.01). Compared with the HM group, the contents of TG, TC and LDL-C in serum and liver homogenate in the HPC-HD and FP groups significantly decreased, and the content of HDL-C significantly increased (P < 0.01); the effect of HPC on lipid content in serum and liver homogenate was dose-dependent. After prophylactic intervention with HPC, the steatosis of hepatocytes was gradually improved with an increase in the dosage of HPC. Compared with the HM group, the AMPK mRNA expression and p-AMPK protein expression in liver tissues in the HPC-HD and FP groups significantly increased (P < 0.01), and the mRNA and protein expression of SREBP-1c, GPAT1, ACC and FAS significantly decreased (P < 0.01), and the mRNA and protein expression of CPT1 and PGC-1α significantly increased in the liver (P < 0.01). Conclusion: HPC can effectively improve lipid metabolism in hyperlipidemic rats, which may be related to the regulation of the AMPK/SREBP-1c signaling pathway. |
| first_indexed | 2024-03-12T00:41:37Z |
| format | Article |
| id | doaj.art-cecc4ce4a91141a6b514a7a2fc722658 |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-03-12T00:41:37Z |
| publishDate | 2023-08-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-cecc4ce4a91141a6b514a7a2fc7226582023-09-15T07:43:31ZengChina Food Publishing CompanyShipin Kexue1002-66302023-08-01441512913610.7506/spkx1002-6630-20220823-281Hawthorn Procyanidins Regulate Lipid Metabolism through the AMPK/SREBP-1c Signaling PathwayMI Wei, YU Min, LI Ning, SHI Tala, CHEN Caiyun0(1. School of Public Health and Management, Binzhou Medical University, Yantai 264003, China;2. School of Pharmacy, Binzhou Medical College, Yantai 264003, China)Objective: To investigate the effect of hawthorn procyanidins (HPC) on lipid metabolism and its mechanism by regulating the AMP-activated protein kinase/sterol regulatory element binding protein-1c (AMPK/SREBP-1c) signaling pathway. Methods: Sixty rats were randomly divided into six groups (n = 10) blank control (BC), hyperlipidemia model (HM), low-, medium-, and high-dose HPC (HPC-LD, HPC-MD, and HPC-HD) and fenofibrate positive control (FP). The contents of triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) in the serum and liver homogenate were measured by commercial kits. Hematoxylin eosin (HE) staining was used to observe hepatic histopathological changes. The mRNA expression levels of AMPK, SREBP-1c, glycerol-3-phosphate acyltransferase-1 (GPAT1), acetyl CoA carboxylase (ACC), fatty acid synthase (FAS), carnitine palmitoyltransterase-1 (CPT1) and peroxisome proliferator activated receptor γ coactivator-1α (PGC-1α) in the liver were measured by quantitative real-time polymerase chain reaction (PCR). Western blotting was used to detect the protein expression of p-AMPK, SREBP-1c, GPAT1, ACC, FAS, CPT1 and PGC-1α in the liver. Results: After seven weeks of feeding on a high-fat diet, body mass, liver mass and liver index significantly increased compared with the control group fed on a basal diet (P < 0.05 or < 0.01). Compared with the HM group, the contents of TG, TC and LDL-C in serum and liver homogenate in the HPC-HD and FP groups significantly decreased, and the content of HDL-C significantly increased (P < 0.01); the effect of HPC on lipid content in serum and liver homogenate was dose-dependent. After prophylactic intervention with HPC, the steatosis of hepatocytes was gradually improved with an increase in the dosage of HPC. Compared with the HM group, the AMPK mRNA expression and p-AMPK protein expression in liver tissues in the HPC-HD and FP groups significantly increased (P < 0.01), and the mRNA and protein expression of SREBP-1c, GPAT1, ACC and FAS significantly decreased (P < 0.01), and the mRNA and protein expression of CPT1 and PGC-1α significantly increased in the liver (P < 0.01). Conclusion: HPC can effectively improve lipid metabolism in hyperlipidemic rats, which may be related to the regulation of the AMPK/SREBP-1c signaling pathway.https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-15-015.pdfhawthorn procyanidins; hyperlipidemia; amp-activated protein kinase (ampk); sterol regulatory element binding protein-1c (srebp-1c) |
| spellingShingle | MI Wei, YU Min, LI Ning, SHI Tala, CHEN Caiyun Hawthorn Procyanidins Regulate Lipid Metabolism through the AMPK/SREBP-1c Signaling Pathway Shipin Kexue hawthorn procyanidins; hyperlipidemia; amp-activated protein kinase (ampk); sterol regulatory element binding protein-1c (srebp-1c) |
| title | Hawthorn Procyanidins Regulate Lipid Metabolism through the AMPK/SREBP-1c Signaling Pathway |
| title_full | Hawthorn Procyanidins Regulate Lipid Metabolism through the AMPK/SREBP-1c Signaling Pathway |
| title_fullStr | Hawthorn Procyanidins Regulate Lipid Metabolism through the AMPK/SREBP-1c Signaling Pathway |
| title_full_unstemmed | Hawthorn Procyanidins Regulate Lipid Metabolism through the AMPK/SREBP-1c Signaling Pathway |
| title_short | Hawthorn Procyanidins Regulate Lipid Metabolism through the AMPK/SREBP-1c Signaling Pathway |
| title_sort | hawthorn procyanidins regulate lipid metabolism through the ampk srebp 1c signaling pathway |
| topic | hawthorn procyanidins; hyperlipidemia; amp-activated protein kinase (ampk); sterol regulatory element binding protein-1c (srebp-1c) |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-15-015.pdf |
| work_keys_str_mv | AT miweiyuminliningshitalachencaiyun hawthornprocyanidinsregulatelipidmetabolismthroughtheampksrebp1csignalingpathway |