Partial characterization of amylases of two indigenous Central Amazonian rhizobia strains
Amylase production and partial characterization of crude enzyme preparations from two rhizobia strains (R-926 and R-991) were evaluated. For both the strains, maximal amylase activities were achieved during the early-to-mid- exponential growth phase; both were active over a pH range from 4.5 to 8.5...
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Instituto de Tecnologia do Paraná (Tecpar)
2010-02-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100005 |
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| author | Arlem Nascimento de Oliveira Luiz Antonio de Oliveira Jerusa Souza Andrade |
| author_facet | Arlem Nascimento de Oliveira Luiz Antonio de Oliveira Jerusa Souza Andrade |
| author_sort | Arlem Nascimento de Oliveira |
| collection | DOAJ |
| description | Amylase production and partial characterization of crude enzyme preparations from two rhizobia strains (R-926 and R-991) were evaluated. For both the strains, maximal amylase activities were achieved during the early-to-mid- exponential growth phase; both were active over a pH range from 4.5 to 8.5 and temperature from 30 to 50 ºC. None of the ions studied (K+, Na+, Ca2+, Hg2+, Mg2+, Mn2+, Cu2+ and Zn2+) was required for the catalytic activity of strain R-926; amylase activity of strain R-991 was stimulated in the presence of K+, Hg2+ and Zn2+. The surfactants SDS, Triton X-100 and Tween-80 did not have a pronounced inhibitory effect on enzyme activities; SDS and Tween-80 caused the highest stimulatory effects. Amylase activities from the rhizobia strains were reduced by up to 30% in the presence of EDTA; amylase activity of R-926 was also inhibited by HgCl2, suggesting that Ca2+and cysteine residues could be important for activity of this strain.<br>A produção e parcial caracterização de extratos brutos de amilase de duas estirpes de rizóbio (R-926 e R-991) foram avaliadas. Para ambas as estirpes, as máximas atividades amilolíticas foram obtidas no início/meio da fase exponencial de crescimento. As amilases rizobiais foram ativas numa variação de pH de 4,5 a 8,5 e temperatura de 30 a 50 ºC. Nenhum dos íons testados (K+, Na+, Ca2+, Hg2+, Mg2+, Mn2+, Cu2+ e Zn2+) foi exigido para a atividade catalítica da estirpe R-926. A amilase produzida pelo R991 foi estimulada na presença de K+, Hg2+ e Zn2+. Os surfactantes SDS, Triton X-100 e Tween-80 não exerceram um pronunciado efeito inibitório sobre as atividades enzimáticas, e SDS e Tween-80 causaram os maiores efeitos estimulatórios. A atividade amilolítica rizobial foi reduzida em até 30% na presença de EDTA; a amilase produzida pela estirpe R-926 também foi inibida pelo HgCl2, sugerindo, ao menos em parte, a importância de Ca2+ e resíduos de cisteína na atividade amilolítica dessa estirpe. |
| first_indexed | 2024-04-14T02:33:41Z |
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| id | doaj.art-ced030f4ec454445abfe07a2d02ac510 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-04-14T02:33:41Z |
| publishDate | 2010-02-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
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| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-ced030f4ec454445abfe07a2d02ac5102022-12-22T02:17:36ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242010-02-01531354510.1590/S1516-89132010000100005Partial characterization of amylases of two indigenous Central Amazonian rhizobia strainsArlem Nascimento de OliveiraLuiz Antonio de OliveiraJerusa Souza AndradeAmylase production and partial characterization of crude enzyme preparations from two rhizobia strains (R-926 and R-991) were evaluated. For both the strains, maximal amylase activities were achieved during the early-to-mid- exponential growth phase; both were active over a pH range from 4.5 to 8.5 and temperature from 30 to 50 ºC. None of the ions studied (K+, Na+, Ca2+, Hg2+, Mg2+, Mn2+, Cu2+ and Zn2+) was required for the catalytic activity of strain R-926; amylase activity of strain R-991 was stimulated in the presence of K+, Hg2+ and Zn2+. The surfactants SDS, Triton X-100 and Tween-80 did not have a pronounced inhibitory effect on enzyme activities; SDS and Tween-80 caused the highest stimulatory effects. Amylase activities from the rhizobia strains were reduced by up to 30% in the presence of EDTA; amylase activity of R-926 was also inhibited by HgCl2, suggesting that Ca2+and cysteine residues could be important for activity of this strain.<br>A produção e parcial caracterização de extratos brutos de amilase de duas estirpes de rizóbio (R-926 e R-991) foram avaliadas. Para ambas as estirpes, as máximas atividades amilolíticas foram obtidas no início/meio da fase exponencial de crescimento. As amilases rizobiais foram ativas numa variação de pH de 4,5 a 8,5 e temperatura de 30 a 50 ºC. Nenhum dos íons testados (K+, Na+, Ca2+, Hg2+, Mg2+, Mn2+, Cu2+ e Zn2+) foi exigido para a atividade catalítica da estirpe R-926. A amilase produzida pelo R991 foi estimulada na presença de K+, Hg2+ e Zn2+. Os surfactantes SDS, Triton X-100 e Tween-80 não exerceram um pronunciado efeito inibitório sobre as atividades enzimáticas, e SDS e Tween-80 causaram os maiores efeitos estimulatórios. A atividade amilolítica rizobial foi reduzida em até 30% na presença de EDTA; a amilase produzida pela estirpe R-926 também foi inibida pelo HgCl2, sugerindo, ao menos em parte, a importância de Ca2+ e resíduos de cisteína na atividade amilolítica dessa estirpe.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100005RhizobiumBradyrhizobiumAmylase productionEnzymatic properties |
| spellingShingle | Arlem Nascimento de Oliveira Luiz Antonio de Oliveira Jerusa Souza Andrade Partial characterization of amylases of two indigenous Central Amazonian rhizobia strains Brazilian Archives of Biology and Technology Rhizobium Bradyrhizobium Amylase production Enzymatic properties |
| title | Partial characterization of amylases of two indigenous Central Amazonian rhizobia strains |
| title_full | Partial characterization of amylases of two indigenous Central Amazonian rhizobia strains |
| title_fullStr | Partial characterization of amylases of two indigenous Central Amazonian rhizobia strains |
| title_full_unstemmed | Partial characterization of amylases of two indigenous Central Amazonian rhizobia strains |
| title_short | Partial characterization of amylases of two indigenous Central Amazonian rhizobia strains |
| title_sort | partial characterization of amylases of two indigenous central amazonian rhizobia strains |
| topic | Rhizobium Bradyrhizobium Amylase production Enzymatic properties |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100005 |
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