Antibacterial and anti-corona virus (229E) activity of Nigella sativa oil combined with photodynamic therapy based on methylene blue in wound infection: in vitro and in vivo study
Abstract Microbial skin infections, antibiotic resistance, and poor wound healing are major problems, and new treatments are needed. Our study targeted solving this problem with Nigella sativa (NS) oil and photodynamic therapy based on methylene blue (MB-PDT). Antibacterial activity and minimum inhi...
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-03018-1 |
| _version_ | 1797453709295222784 |
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| author | Sahar E. Abo-Neima Mostafa M. El-Sheekh Mayasar I. Al-Zaban Abeer I. M. EL-Sayed |
| author_facet | Sahar E. Abo-Neima Mostafa M. El-Sheekh Mayasar I. Al-Zaban Abeer I. M. EL-Sayed |
| author_sort | Sahar E. Abo-Neima |
| collection | DOAJ |
| description | Abstract Microbial skin infections, antibiotic resistance, and poor wound healing are major problems, and new treatments are needed. Our study targeted solving this problem with Nigella sativa (NS) oil and photodynamic therapy based on methylene blue (MB-PDT). Antibacterial activity and minimum inhibitory concentration (MIC) were determined via agar well diffusion assay and broth microdilution, respectively. Transmission electron microscopy (TEM) proved deformations in Staphylococcus aureus ATCC 6538. Gas chromatography–mass spectrometry identified useful compounds that were suggested to be responsible for the potency of the oil. NS oil was tested as an antivirus against low pathogenic coronavirus (229E). Therapies examined, MB-PDT, NS, and MB-PDT + NS oil, to accelerate wound healing. The antibacterial efficacy against S. aureus was promising, with a MIC of 12.5% and TEM showing injured cells treated with NS oil. This oil inhibited 229E virus up to 42.85% and 32.14%. All tested therapies were successful in accelerating wound healing. The most successful was combined therapy (MB-PDT + NS oil), with a faster healing time. The combined therapy (MB-PDT + NS oil) reduced bacterial counts, which may be a key factor in accelerating wound healing. Skin wound histology was investigated; blood hematology and biochemical analysis did not change significantly after the safe combination treatment. A combination treatment could facilitate healing in a simple and inexpensive way in the future. Based on the results of the in vitro and in vivo studies, it was determined that NS oil had antibacterial and anti-corona virus activity when used in conjunction with photodynamic treatment based on methylene blue to treat wound infections. |
| first_indexed | 2024-03-09T15:26:42Z |
| format | Article |
| id | doaj.art-ced42ad55a4746f9af4cac8571da3abc |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-09T15:26:42Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-ced42ad55a4746f9af4cac8571da3abc2023-11-26T12:30:23ZengBMCBMC Microbiology1471-21802023-09-0123111810.1186/s12866-023-03018-1Antibacterial and anti-corona virus (229E) activity of Nigella sativa oil combined with photodynamic therapy based on methylene blue in wound infection: in vitro and in vivo studySahar E. Abo-Neima0Mostafa M. El-Sheekh1Mayasar I. Al-Zaban2Abeer I. M. EL-Sayed3Physics Department, Faculty of Science, Damanhour UniversityBotany Department, Faculty of Science, Tanta UniversityDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman UniversityBotany and Microbiology Department, Faculty of Science, Damanhour UniversityAbstract Microbial skin infections, antibiotic resistance, and poor wound healing are major problems, and new treatments are needed. Our study targeted solving this problem with Nigella sativa (NS) oil and photodynamic therapy based on methylene blue (MB-PDT). Antibacterial activity and minimum inhibitory concentration (MIC) were determined via agar well diffusion assay and broth microdilution, respectively. Transmission electron microscopy (TEM) proved deformations in Staphylococcus aureus ATCC 6538. Gas chromatography–mass spectrometry identified useful compounds that were suggested to be responsible for the potency of the oil. NS oil was tested as an antivirus against low pathogenic coronavirus (229E). Therapies examined, MB-PDT, NS, and MB-PDT + NS oil, to accelerate wound healing. The antibacterial efficacy against S. aureus was promising, with a MIC of 12.5% and TEM showing injured cells treated with NS oil. This oil inhibited 229E virus up to 42.85% and 32.14%. All tested therapies were successful in accelerating wound healing. The most successful was combined therapy (MB-PDT + NS oil), with a faster healing time. The combined therapy (MB-PDT + NS oil) reduced bacterial counts, which may be a key factor in accelerating wound healing. Skin wound histology was investigated; blood hematology and biochemical analysis did not change significantly after the safe combination treatment. A combination treatment could facilitate healing in a simple and inexpensive way in the future. Based on the results of the in vitro and in vivo studies, it was determined that NS oil had antibacterial and anti-corona virus activity when used in conjunction with photodynamic treatment based on methylene blue to treat wound infections.https://doi.org/10.1186/s12866-023-03018-1Anti-coronavirus (229E)Antibacterial activityGas chromatographyLow-intensity laser therapyNigella sativaPhotodynamic therapy |
| spellingShingle | Sahar E. Abo-Neima Mostafa M. El-Sheekh Mayasar I. Al-Zaban Abeer I. M. EL-Sayed Antibacterial and anti-corona virus (229E) activity of Nigella sativa oil combined with photodynamic therapy based on methylene blue in wound infection: in vitro and in vivo study BMC Microbiology Anti-coronavirus (229E) Antibacterial activity Gas chromatography Low-intensity laser therapy Nigella sativa Photodynamic therapy |
| title | Antibacterial and anti-corona virus (229E) activity of Nigella sativa oil combined with photodynamic therapy based on methylene blue in wound infection: in vitro and in vivo study |
| title_full | Antibacterial and anti-corona virus (229E) activity of Nigella sativa oil combined with photodynamic therapy based on methylene blue in wound infection: in vitro and in vivo study |
| title_fullStr | Antibacterial and anti-corona virus (229E) activity of Nigella sativa oil combined with photodynamic therapy based on methylene blue in wound infection: in vitro and in vivo study |
| title_full_unstemmed | Antibacterial and anti-corona virus (229E) activity of Nigella sativa oil combined with photodynamic therapy based on methylene blue in wound infection: in vitro and in vivo study |
| title_short | Antibacterial and anti-corona virus (229E) activity of Nigella sativa oil combined with photodynamic therapy based on methylene blue in wound infection: in vitro and in vivo study |
| title_sort | antibacterial and anti corona virus 229e activity of nigella sativa oil combined with photodynamic therapy based on methylene blue in wound infection in vitro and in vivo study |
| topic | Anti-coronavirus (229E) Antibacterial activity Gas chromatography Low-intensity laser therapy Nigella sativa Photodynamic therapy |
| url | https://doi.org/10.1186/s12866-023-03018-1 |
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