Application of Extracellular Polymeric Substances during Cultivation of Microalgae Biomass
Extracellular polymeric substances (EPS) produced by microorganisms contain polymers that are used for the bioflocculation of microalgae; however, these polymers are also organic compounds that might be used as carbon sources. The study analyzed two strategies for the introduction of EPS for <i&g...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/13/19/10796 |
| _version_ | 1797576207412232192 |
|---|---|
| author | Paulina Rusanowska Marcin Zieliński Magda Dudek Marcin Dębowski |
| author_facet | Paulina Rusanowska Marcin Zieliński Magda Dudek Marcin Dębowski |
| author_sort | Paulina Rusanowska |
| collection | DOAJ |
| description | Extracellular polymeric substances (EPS) produced by microorganisms contain polymers that are used for the bioflocculation of microalgae; however, these polymers are also organic compounds that might be used as carbon sources. The study analyzed two strategies for the introduction of EPS for <i>Tetraselmis subcordiformis</i>, <i>Chlorella</i> sp., and <i>Arthrospira platensis</i> biomass harvesting. In the first variant, EPS in the dose of 100 mg TOC/g were added to the photobioreactor every other day from the beginning of the cultivation, while in the second variant, EPS in the two doses of 100 mg TOC/g and 300 mg TOC/g were only added at the end of cultivation. In the first variant, the results proved that microalgae/cyanobacteria can use the EPS as external carbon sources. The cultures were characterized by a faster increase in biomass concentration, which contained less chlorophyll. However, the EPS content did not change. In the second variant, the addition of EPS did not affect the EPS content and the sedimentation of the <i>Chlorella</i> sp. biomass. The biomass of <i>T. subcordiformis</i> was characterized by a much better sedimentation coefficient. The greatest differences were observed in the <i>A. platensis</i> culture: the biomass concentration increased from 1.2 ± 0.2 g/L to 1.9 ± 0.2 g/L, EPS content increased by 16%, and sedimentation efficiency increased to 72%. |
| first_indexed | 2024-03-10T21:49:57Z |
| format | Article |
| id | doaj.art-ced553e281134eae87787406170a44d4 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T21:49:57Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-ced553e281134eae87787406170a44d42023-11-19T14:04:20ZengMDPI AGApplied Sciences2076-34172023-09-0113191079610.3390/app131910796Application of Extracellular Polymeric Substances during Cultivation of Microalgae BiomassPaulina Rusanowska0Marcin Zieliński1Magda Dudek2Marcin Dębowski3Department of Environmental Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, 10-720 Olsztyn, PolandDepartment of Environmental Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, 10-720 Olsztyn, PolandDepartment of Environmental Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, 10-720 Olsztyn, PolandDepartment of Environmental Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, 10-720 Olsztyn, PolandExtracellular polymeric substances (EPS) produced by microorganisms contain polymers that are used for the bioflocculation of microalgae; however, these polymers are also organic compounds that might be used as carbon sources. The study analyzed two strategies for the introduction of EPS for <i>Tetraselmis subcordiformis</i>, <i>Chlorella</i> sp., and <i>Arthrospira platensis</i> biomass harvesting. In the first variant, EPS in the dose of 100 mg TOC/g were added to the photobioreactor every other day from the beginning of the cultivation, while in the second variant, EPS in the two doses of 100 mg TOC/g and 300 mg TOC/g were only added at the end of cultivation. In the first variant, the results proved that microalgae/cyanobacteria can use the EPS as external carbon sources. The cultures were characterized by a faster increase in biomass concentration, which contained less chlorophyll. However, the EPS content did not change. In the second variant, the addition of EPS did not affect the EPS content and the sedimentation of the <i>Chlorella</i> sp. biomass. The biomass of <i>T. subcordiformis</i> was characterized by a much better sedimentation coefficient. The greatest differences were observed in the <i>A. platensis</i> culture: the biomass concentration increased from 1.2 ± 0.2 g/L to 1.9 ± 0.2 g/L, EPS content increased by 16%, and sedimentation efficiency increased to 72%.https://www.mdpi.com/2076-3417/13/19/10796biomass harvestingEPSbioflocculationmixotrophic growth |
| spellingShingle | Paulina Rusanowska Marcin Zieliński Magda Dudek Marcin Dębowski Application of Extracellular Polymeric Substances during Cultivation of Microalgae Biomass Applied Sciences biomass harvesting EPS bioflocculation mixotrophic growth |
| title | Application of Extracellular Polymeric Substances during Cultivation of Microalgae Biomass |
| title_full | Application of Extracellular Polymeric Substances during Cultivation of Microalgae Biomass |
| title_fullStr | Application of Extracellular Polymeric Substances during Cultivation of Microalgae Biomass |
| title_full_unstemmed | Application of Extracellular Polymeric Substances during Cultivation of Microalgae Biomass |
| title_short | Application of Extracellular Polymeric Substances during Cultivation of Microalgae Biomass |
| title_sort | application of extracellular polymeric substances during cultivation of microalgae biomass |
| topic | biomass harvesting EPS bioflocculation mixotrophic growth |
| url | https://www.mdpi.com/2076-3417/13/19/10796 |
| work_keys_str_mv | AT paulinarusanowska applicationofextracellularpolymericsubstancesduringcultivationofmicroalgaebiomass AT marcinzielinski applicationofextracellularpolymericsubstancesduringcultivationofmicroalgaebiomass AT magdadudek applicationofextracellularpolymericsubstancesduringcultivationofmicroalgaebiomass AT marcindebowski applicationofextracellularpolymericsubstancesduringcultivationofmicroalgaebiomass |