ANTIMICROBIALACTIVITY OF THE LACTIC ACID BACTERIAAND CAROTOVORICENES COMPLEX AGAINST THE SOFT ROT CAUSATIVE AGENT DURING THE STORAGE
Aim. To determine correlation between the storage conditions and the activity of complex from Erwinia carotovora ОНУ320 carotovoricnes and Lactobacillus plantarum ONU87 active against phytopathogenic erwinias. Materials and methods. The complex consists of carotovoricines (defective phage particles)...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Odessa I. I. Mechnikov National University
2017-03-01
|
| Series: | Mìkrobìologìâ ì Bìotehnologìâ |
| Subjects: | |
| Online Access: | http://mbt.onu.edu.ua/article/view/96325 |
| _version_ | 1818914966378381312 |
|---|---|
| author | К. Д. Крилова Ж. Ю. Сергєєва О. В. Басюл |
| author_facet | К. Д. Крилова Ж. Ю. Сергєєва О. В. Басюл |
| author_sort | К. Д. Крилова |
| collection | DOAJ |
| description | Aim. To determine correlation between the storage conditions and the activity of complex from Erwinia carotovora ОНУ320 carotovoricnes and Lactobacillus plantarum ONU87 active against phytopathogenic erwinias. Materials and methods. The complex consists of carotovoricines (defective phage particles) from the strain E. carotovora ОНУ320, obtained by spontaneous induction, and of bacteria L. plantarum ONU87 (cells titer – 105 CFU/ml) in 1:1. The complex and each component separately were stored for 31 days at -20, 4 and 20 °C. Stability control of the individual components of the mixture (bacteriocins killer activity and lactobacilli cells titer determination) was performed during all term of storage. Carotovoricines killer activity was determined according to size and diameter of lysing zones. L. plantarum ОНУ87 viability was determined as titer after the growth on MRS agar. Results. For the first time the optimal storage conditions for the mixture of L. plantarum ONU87 live cells and E. carotovora ОНУ320 carotovoricines were determibed. The negative influence of L. plantarum ONU87 strain on the activity of carotovoricine mixture component was shown, if it was stored at temperature 20 and 4 °C. Conclusions. The decrease of carotovoricines activity after the storage at 4 and 20 °С with lactobacilli possibly occurs because of destabilization of proteins that form defective phage tails and theirs selfcontraction. |
| first_indexed | 2024-12-19T23:54:47Z |
| format | Article |
| id | doaj.art-ced5707166134330aa82c0723be8180e |
| institution | Directory Open Access Journal |
| issn | 2076-0558 2307-4663 |
| language | English |
| last_indexed | 2024-12-19T23:54:47Z |
| publishDate | 2017-03-01 |
| publisher | Odessa I. I. Mechnikov National University |
| record_format | Article |
| series | Mìkrobìologìâ ì Bìotehnologìâ |
| spelling | doaj.art-ced5707166134330aa82c0723be8180e2022-12-21T20:01:02ZengOdessa I. I. Mechnikov National UniversityMìkrobìologìâ ì Bìotehnologìâ2076-05582307-46632017-03-0101(37)859310.18524/2307-4663.2017.1(37).9632596325ANTIMICROBIALACTIVITY OF THE LACTIC ACID BACTERIAAND CAROTOVORICENES COMPLEX AGAINST THE SOFT ROT CAUSATIVE AGENT DURING THE STORAGEК. Д. Крилова0Ж. Ю. Сергєєва1О. В. Басюл2Одеський національний університет імені І.І. МечниковаОдеський національний університет імені І.І. МечниковаОдеський національний університет імені І.І. МечниковаAim. To determine correlation between the storage conditions and the activity of complex from Erwinia carotovora ОНУ320 carotovoricnes and Lactobacillus plantarum ONU87 active against phytopathogenic erwinias. Materials and methods. The complex consists of carotovoricines (defective phage particles) from the strain E. carotovora ОНУ320, obtained by spontaneous induction, and of bacteria L. plantarum ONU87 (cells titer – 105 CFU/ml) in 1:1. The complex and each component separately were stored for 31 days at -20, 4 and 20 °C. Stability control of the individual components of the mixture (bacteriocins killer activity and lactobacilli cells titer determination) was performed during all term of storage. Carotovoricines killer activity was determined according to size and diameter of lysing zones. L. plantarum ОНУ87 viability was determined as titer after the growth on MRS agar. Results. For the first time the optimal storage conditions for the mixture of L. plantarum ONU87 live cells and E. carotovora ОНУ320 carotovoricines were determibed. The negative influence of L. plantarum ONU87 strain on the activity of carotovoricine mixture component was shown, if it was stored at temperature 20 and 4 °C. Conclusions. The decrease of carotovoricines activity after the storage at 4 and 20 °С with lactobacilli possibly occurs because of destabilization of proteins that form defective phage tails and theirs selfcontraction.http://mbt.onu.edu.ua/article/view/96325зберіганняфітопатогенибактеріоцинerwinia carotovoralactobacillus plantarum |
| spellingShingle | К. Д. Крилова Ж. Ю. Сергєєва О. В. Басюл ANTIMICROBIALACTIVITY OF THE LACTIC ACID BACTERIAAND CAROTOVORICENES COMPLEX AGAINST THE SOFT ROT CAUSATIVE AGENT DURING THE STORAGE Mìkrobìologìâ ì Bìotehnologìâ зберігання фітопатогени бактеріоцин erwinia carotovora lactobacillus plantarum |
| title | ANTIMICROBIALACTIVITY OF THE LACTIC ACID BACTERIAAND CAROTOVORICENES COMPLEX AGAINST THE SOFT ROT CAUSATIVE AGENT DURING THE STORAGE |
| title_full | ANTIMICROBIALACTIVITY OF THE LACTIC ACID BACTERIAAND CAROTOVORICENES COMPLEX AGAINST THE SOFT ROT CAUSATIVE AGENT DURING THE STORAGE |
| title_fullStr | ANTIMICROBIALACTIVITY OF THE LACTIC ACID BACTERIAAND CAROTOVORICENES COMPLEX AGAINST THE SOFT ROT CAUSATIVE AGENT DURING THE STORAGE |
| title_full_unstemmed | ANTIMICROBIALACTIVITY OF THE LACTIC ACID BACTERIAAND CAROTOVORICENES COMPLEX AGAINST THE SOFT ROT CAUSATIVE AGENT DURING THE STORAGE |
| title_short | ANTIMICROBIALACTIVITY OF THE LACTIC ACID BACTERIAAND CAROTOVORICENES COMPLEX AGAINST THE SOFT ROT CAUSATIVE AGENT DURING THE STORAGE |
| title_sort | antimicrobialactivity of the lactic acid bacteriaand carotovoricenes complex against the soft rot causative agent during the storage |
| topic | зберігання фітопатогени бактеріоцин erwinia carotovora lactobacillus plantarum |
| url | http://mbt.onu.edu.ua/article/view/96325 |
| work_keys_str_mv | AT kdkrilova antimicrobialactivityofthelacticacidbacteriaandcarotovoricenescomplexagainstthesoftrotcausativeagentduringthestorage AT žûsergêêva antimicrobialactivityofthelacticacidbacteriaandcarotovoricenescomplexagainstthesoftrotcausativeagentduringthestorage AT ovbasûl antimicrobialactivityofthelacticacidbacteriaandcarotovoricenescomplexagainstthesoftrotcausativeagentduringthestorage |