E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway
IntroductionColon cancer is one of the most prevalent malignancies and causes of cancer-related deaths worldwide. Thus, further research is required to explicate the latent molecular mechanisms and look for novel biomarkers. E2F3 has been confirmed to be an oncogene in a variety of cancers. However,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2023.1203712/full |
| _version_ | 1797791545002295296 |
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| author | Qingkun Gao Ke An Zhe Lv Yanzhao Wang Changmin Ding Wensheng Huang |
| author_facet | Qingkun Gao Ke An Zhe Lv Yanzhao Wang Changmin Ding Wensheng Huang |
| author_sort | Qingkun Gao |
| collection | DOAJ |
| description | IntroductionColon cancer is one of the most prevalent malignancies and causes of cancer-related deaths worldwide. Thus, further research is required to explicate the latent molecular mechanisms and look for novel biomarkers. E2F3 has been confirmed to be an oncogene in a variety of cancers. However, the particular regulation of E2F3 in colon cancer needs further investigation.MethodsThe self-renewal ability was detected through a sphere formation assay. The tumorigenic ability was measured through nude mice in vivo assay. The protein expression of genes was examined through a Western blot. The expression of E2F3 in tumor tissues was detected through an IHC assay. The resistance to cisplatin was assessed through the CCK-8 assay. The cell migration and invasion abilities were measured after upregulating or suppressing E2F3 through the Transwell assay.ResultsResults uncovered that E2F3 was upregulated in spheroid cells. In addition, E2F3 facilitates stemness in colon cancer. Moreover, E2F3 facilitated colon cancer cell migration and invasion. Finally, it was revealed that E2F3 affected the STAT3 pathway to modulate stemness in colon cancer. E2F3 served as a promoter regulator in colon cancer, aggravating tumorigenesis and stemness in colon cancer progression through the STAT3 pathway.ConclusionE2F3 may be a useful biomarker for anticancer treatment in colon cancer. |
| first_indexed | 2024-03-13T02:20:22Z |
| format | Article |
| id | doaj.art-ced59e5faef7499995afb3394ac4e218 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-03-13T02:20:22Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-ced59e5faef7499995afb3394ac4e2182023-06-30T10:24:11ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2023-06-011310.3389/fonc.2023.12037121203712E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathwayQingkun GaoKe AnZhe LvYanzhao WangChangmin DingWensheng HuangIntroductionColon cancer is one of the most prevalent malignancies and causes of cancer-related deaths worldwide. Thus, further research is required to explicate the latent molecular mechanisms and look for novel biomarkers. E2F3 has been confirmed to be an oncogene in a variety of cancers. However, the particular regulation of E2F3 in colon cancer needs further investigation.MethodsThe self-renewal ability was detected through a sphere formation assay. The tumorigenic ability was measured through nude mice in vivo assay. The protein expression of genes was examined through a Western blot. The expression of E2F3 in tumor tissues was detected through an IHC assay. The resistance to cisplatin was assessed through the CCK-8 assay. The cell migration and invasion abilities were measured after upregulating or suppressing E2F3 through the Transwell assay.ResultsResults uncovered that E2F3 was upregulated in spheroid cells. In addition, E2F3 facilitates stemness in colon cancer. Moreover, E2F3 facilitated colon cancer cell migration and invasion. Finally, it was revealed that E2F3 affected the STAT3 pathway to modulate stemness in colon cancer. E2F3 served as a promoter regulator in colon cancer, aggravating tumorigenesis and stemness in colon cancer progression through the STAT3 pathway.ConclusionE2F3 may be a useful biomarker for anticancer treatment in colon cancer.https://www.frontiersin.org/articles/10.3389/fonc.2023.1203712/fullE2F3stemnessSTAT3 pathwaycolon cancercancer |
| spellingShingle | Qingkun Gao Ke An Zhe Lv Yanzhao Wang Changmin Ding Wensheng Huang E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway Frontiers in Oncology E2F3 stemness STAT3 pathway colon cancer cancer |
| title | E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway |
| title_full | E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway |
| title_fullStr | E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway |
| title_full_unstemmed | E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway |
| title_short | E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway |
| title_sort | e2f3 accelerates the stemness of colon cancer cells by activating the stat3 pathway |
| topic | E2F3 stemness STAT3 pathway colon cancer cancer |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2023.1203712/full |
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