Placenta-Specific miR-125b Overexpression Leads to Increased Rates of Pregnancy Loss in Mice
Pregnancy loss (PL) is one of the common complications that women can experience during pregnancy, with an occurrence rate of 1 to 5%. The potential causes of pregnancy loss are unclear, with no effective treatment modalities being available. It has been previously reported that the level of miR-125...
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MDPI AG
2022-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/2/943 |
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| author | Fen Sun Hui Cai Lunbo Tan Dezhe Qin Jian Zhang Jinlian Hua Xiujun Fan Sha Peng |
| author_facet | Fen Sun Hui Cai Lunbo Tan Dezhe Qin Jian Zhang Jinlian Hua Xiujun Fan Sha Peng |
| author_sort | Fen Sun |
| collection | DOAJ |
| description | Pregnancy loss (PL) is one of the common complications that women can experience during pregnancy, with an occurrence rate of 1 to 5%. The potential causes of pregnancy loss are unclear, with no effective treatment modalities being available. It has been previously reported that the level of miR-125b was significantly increased in placentas of PL patients. However, the role of miR-125b in the development of PL still remains unknown. In the current study, an miR-125b placenta-specific over-expression model was constructed by lentiviral transfecting zona-free mouse embryos followed by embryo transfer. On gestation day 15, it was observed that the placenta was significantly smaller in the miR-125b placenta-specific overexpression group than the control group. Additionally, the abortion rate of the miR-125b placenta-specific overexpression group was markedly higher than in the control group. The blood vessel diameter was larger in the miR-125b-overexpressing specific placenta. In addition, miR-125b-overexpressing HTR8 and JEG3 cell lines were also generated to analyze the migration and invasion ability of trophoblasts. The results showed that miR-125b overexpression significantly suppressed the migration and invasion ability of HTR8 and JEG3 cells. Overall, our results demonstrated that miR-125b can affect embryo implantation through modulating placenta angiogenesis and trophoblast cell invasion capacity that can lead to PL. |
| first_indexed | 2024-03-10T01:17:29Z |
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| id | doaj.art-ced85ea0109946ebb4bc6165b774527b |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:17:29Z |
| publishDate | 2022-01-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ced85ea0109946ebb4bc6165b774527b2023-11-23T14:06:58ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-01-0123294310.3390/ijms23020943Placenta-Specific miR-125b Overexpression Leads to Increased Rates of Pregnancy Loss in MiceFen Sun0Hui Cai1Lunbo Tan2Dezhe Qin3Jian Zhang4Jinlian Hua5Xiujun Fan6Sha Peng7Shaanxi Centre of Stem Cells Engineering & Technology, College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang 712100, ChinaShaanxi Centre of Stem Cells Engineering & Technology, College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang 712100, ChinaLaboratory of Reproductive Health, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, ChinaShaanxi Centre of Stem Cells Engineering & Technology, College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang 712100, ChinaCenter for Reproduction and Health Development, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, ChinaShaanxi Centre of Stem Cells Engineering & Technology, College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang 712100, ChinaCenter for Reproduction and Health Development, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, ChinaShaanxi Centre of Stem Cells Engineering & Technology, College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang 712100, ChinaPregnancy loss (PL) is one of the common complications that women can experience during pregnancy, with an occurrence rate of 1 to 5%. The potential causes of pregnancy loss are unclear, with no effective treatment modalities being available. It has been previously reported that the level of miR-125b was significantly increased in placentas of PL patients. However, the role of miR-125b in the development of PL still remains unknown. In the current study, an miR-125b placenta-specific over-expression model was constructed by lentiviral transfecting zona-free mouse embryos followed by embryo transfer. On gestation day 15, it was observed that the placenta was significantly smaller in the miR-125b placenta-specific overexpression group than the control group. Additionally, the abortion rate of the miR-125b placenta-specific overexpression group was markedly higher than in the control group. The blood vessel diameter was larger in the miR-125b-overexpressing specific placenta. In addition, miR-125b-overexpressing HTR8 and JEG3 cell lines were also generated to analyze the migration and invasion ability of trophoblasts. The results showed that miR-125b overexpression significantly suppressed the migration and invasion ability of HTR8 and JEG3 cells. Overall, our results demonstrated that miR-125b can affect embryo implantation through modulating placenta angiogenesis and trophoblast cell invasion capacity that can lead to PL.https://www.mdpi.com/1422-0067/23/2/943pregnancy lossangiogenesisHTR8 cellsJEG3 cells<i>VEGF</i> |
| spellingShingle | Fen Sun Hui Cai Lunbo Tan Dezhe Qin Jian Zhang Jinlian Hua Xiujun Fan Sha Peng Placenta-Specific miR-125b Overexpression Leads to Increased Rates of Pregnancy Loss in Mice International Journal of Molecular Sciences pregnancy loss angiogenesis HTR8 cells JEG3 cells <i>VEGF</i> |
| title | Placenta-Specific miR-125b Overexpression Leads to Increased Rates of Pregnancy Loss in Mice |
| title_full | Placenta-Specific miR-125b Overexpression Leads to Increased Rates of Pregnancy Loss in Mice |
| title_fullStr | Placenta-Specific miR-125b Overexpression Leads to Increased Rates of Pregnancy Loss in Mice |
| title_full_unstemmed | Placenta-Specific miR-125b Overexpression Leads to Increased Rates of Pregnancy Loss in Mice |
| title_short | Placenta-Specific miR-125b Overexpression Leads to Increased Rates of Pregnancy Loss in Mice |
| title_sort | placenta specific mir 125b overexpression leads to increased rates of pregnancy loss in mice |
| topic | pregnancy loss angiogenesis HTR8 cells JEG3 cells <i>VEGF</i> |
| url | https://www.mdpi.com/1422-0067/23/2/943 |
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