Evaluation of the Effects of Thymoquinone on Oxidative Stress in A549 Lung Cancer Cell Line
Background: Thymoquinone (TQ), an active part of Nigella sativa, has been reported as an anticancer agent. This study aimed to evaluate different anticancer effects of TQ on oxidative stress markers and Peroxiredoxin 4 (P4) in lung cancer A549 cell line. Method: In this experimental study, we used T...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shiraz University of Medical Sciences
2023-04-01
|
| Series: | Middle East Journal of Cancer |
| Subjects: | |
| Online Access: | https://mejc.sums.ac.ir/article_48026_72288e1fbc9491f3b1347aec53fae135.pdf |
| _version_ | 1797846184460550144 |
|---|---|
| author | Reza Farsiabi Iraj Khodadadi Jamshid Karimi Gholamreza Shafiee |
| author_facet | Reza Farsiabi Iraj Khodadadi Jamshid Karimi Gholamreza Shafiee |
| author_sort | Reza Farsiabi |
| collection | DOAJ |
| description | Background: Thymoquinone (TQ), an active part of Nigella sativa, has been reported as an anticancer agent. This study aimed to evaluate different anticancer effects of TQ on oxidative stress markers and Peroxiredoxin 4 (P4) in lung cancer A549 cell line.
Method: In this experimental study, we used TQ concentrations to treat lung A549 cells and determined cell viability by the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay at 12, 24, and 48 h times. The IC50 concentration of TQ was found with MTT assay. We studied the total antioxidant capacity (TAC) and total oxidant status (TOS) using the manual assay, and analyzed catalase (CAT), superoxide dismutases (SOD), and glutathione peroxidase (GPx) activity using the enzyme-linked immunoassay (ELISA) kits. Moreover, the concentration of peroxiredoxin-4 (PRXD4) was measured with the ELISA Kit.
Results: The IC50 of TQ for A549 cells was calculated to be 40 μM concentration for 24 h of incubation. TAC index significantly decreased in the treated cells compared with the controls (P = 0.05), whereas TOS and PRXD4 levels showed a significant increase (P = 0.05). Additionally, the results showed that the CAT, SOD, and GPX activity enzymes significantly decreased in 20, 40, and 60 μM TQ in comparison with the control cells (P = 0.05).
Conclusion: TQ has significant inhibitory effects on A549 cells and could be utilized in novel therapy not only for lung cancer, but also for other tumors. |
| first_indexed | 2024-04-09T17:50:48Z |
| format | Article |
| id | doaj.art-cede92900b8e4966883951b7ee13ad22 |
| institution | Directory Open Access Journal |
| issn | 2008-6709 2008-6687 |
| language | English |
| last_indexed | 2024-04-09T17:50:48Z |
| publishDate | 2023-04-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Middle East Journal of Cancer |
| spelling | doaj.art-cede92900b8e4966883951b7ee13ad222023-04-16T08:23:55ZengShiraz University of Medical SciencesMiddle East Journal of Cancer2008-67092008-66872023-04-0114223124010.30476/mejc.2021.92306.165348026Evaluation of the Effects of Thymoquinone on Oxidative Stress in A549 Lung Cancer Cell LineReza Farsiabi0Iraj Khodadadi1Jamshid Karimi2Gholamreza Shafiee3Department of Clinical Biochemistry, Medicine School, Hamadan University of Medical Sciences, Hamadan, IranDepartment of Clinical Biochemistry, Medicine School, Hamadan University of Medical Sciences, Hamadan, IranDepartment of Clinical Biochemistry, Medicine School, Hamadan University of Medical Sciences, Hamadan, IranDepartment of Clinical Biochemistry, Medicine School, Hamadan University of Medical Sciences, Hamadan, IranBackground: Thymoquinone (TQ), an active part of Nigella sativa, has been reported as an anticancer agent. This study aimed to evaluate different anticancer effects of TQ on oxidative stress markers and Peroxiredoxin 4 (P4) in lung cancer A549 cell line. Method: In this experimental study, we used TQ concentrations to treat lung A549 cells and determined cell viability by the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay at 12, 24, and 48 h times. The IC50 concentration of TQ was found with MTT assay. We studied the total antioxidant capacity (TAC) and total oxidant status (TOS) using the manual assay, and analyzed catalase (CAT), superoxide dismutases (SOD), and glutathione peroxidase (GPx) activity using the enzyme-linked immunoassay (ELISA) kits. Moreover, the concentration of peroxiredoxin-4 (PRXD4) was measured with the ELISA Kit. Results: The IC50 of TQ for A549 cells was calculated to be 40 μM concentration for 24 h of incubation. TAC index significantly decreased in the treated cells compared with the controls (P = 0.05), whereas TOS and PRXD4 levels showed a significant increase (P = 0.05). Additionally, the results showed that the CAT, SOD, and GPX activity enzymes significantly decreased in 20, 40, and 60 μM TQ in comparison with the control cells (P = 0.05). Conclusion: TQ has significant inhibitory effects on A549 cells and could be utilized in novel therapy not only for lung cancer, but also for other tumors.https://mejc.sums.ac.ir/article_48026_72288e1fbc9491f3b1347aec53fae135.pdfthymoquinoneoxidative stressperoxiredoxin 4lung neoplasms |
| spellingShingle | Reza Farsiabi Iraj Khodadadi Jamshid Karimi Gholamreza Shafiee Evaluation of the Effects of Thymoquinone on Oxidative Stress in A549 Lung Cancer Cell Line Middle East Journal of Cancer thymoquinone oxidative stress peroxiredoxin 4 lung neoplasms |
| title | Evaluation of the Effects of Thymoquinone on Oxidative Stress in A549 Lung Cancer Cell Line |
| title_full | Evaluation of the Effects of Thymoquinone on Oxidative Stress in A549 Lung Cancer Cell Line |
| title_fullStr | Evaluation of the Effects of Thymoquinone on Oxidative Stress in A549 Lung Cancer Cell Line |
| title_full_unstemmed | Evaluation of the Effects of Thymoquinone on Oxidative Stress in A549 Lung Cancer Cell Line |
| title_short | Evaluation of the Effects of Thymoquinone on Oxidative Stress in A549 Lung Cancer Cell Line |
| title_sort | evaluation of the effects of thymoquinone on oxidative stress in a549 lung cancer cell line |
| topic | thymoquinone oxidative stress peroxiredoxin 4 lung neoplasms |
| url | https://mejc.sums.ac.ir/article_48026_72288e1fbc9491f3b1347aec53fae135.pdf |
| work_keys_str_mv | AT rezafarsiabi evaluationoftheeffectsofthymoquinoneonoxidativestressina549lungcancercellline AT irajkhodadadi evaluationoftheeffectsofthymoquinoneonoxidativestressina549lungcancercellline AT jamshidkarimi evaluationoftheeffectsofthymoquinoneonoxidativestressina549lungcancercellline AT gholamrezashafiee evaluationoftheeffectsofthymoquinoneonoxidativestressina549lungcancercellline |