Antioxidant, Antimicrobial, and Bioactive Potential of Two New Haloarchaeal Strains Isolated from Odiel Salterns (Southwest Spain)
The need to survive in extreme environments has furnished haloarchaea with a series of components specially adapted to work in such conditions. The possible application of these molecules in the pharmaceutical and industrial fields has received increasing attention; however, many potential bioactivi...
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MDPI AG
2020-09-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/9/9/298 |
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| author | Patricia Gómez-Villegas Javier Vigara Marta Vila João Varela Luísa Barreira Rosa Léon |
| author_facet | Patricia Gómez-Villegas Javier Vigara Marta Vila João Varela Luísa Barreira Rosa Léon |
| author_sort | Patricia Gómez-Villegas |
| collection | DOAJ |
| description | The need to survive in extreme environments has furnished haloarchaea with a series of components specially adapted to work in such conditions. The possible application of these molecules in the pharmaceutical and industrial fields has received increasing attention; however, many potential bioactivities of haloarchaea are still poorly explored. In this paper, we describe the isolation and identification of two new haloarchaeal strains from the saltern ponds located in the marshlands of the Odiel River, in the southwest of Spain, as well as the in vitro assessment of their antioxidant, antimicrobial, and bioactive properties. The acetone extract obtained from the new isolated <i>Haloarcula</i> strain exhibited the highest antioxidant activity, while the acetone extracts from both isolated strains demonstrated a strong antimicrobial activity, especially against other halophilic microorganisms. Moreover, these extracts showed a remarkable ability to inhibit the enzyme cyclooxygenase-2 and to activate the melanogenic enzyme tyrosinase, indicating their potential against chronic inflammation and skin pigmentation disorders. Finally, the aqueous protein-rich extracts obtained from both haloarchaea exhibited an important inhibitory effect on the activity of the acetylcholinesterase enzyme, involved in the hydrolysis of cholinergic neurotransmitters and related to several neurological diseases. |
| first_indexed | 2024-03-10T16:13:01Z |
| format | Article |
| id | doaj.art-cee0fb758310403384ae432371da566b |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T16:13:01Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-cee0fb758310403384ae432371da566b2023-11-20T14:18:08ZengMDPI AGBiology2079-77372020-09-019929810.3390/biology9090298Antioxidant, Antimicrobial, and Bioactive Potential of Two New Haloarchaeal Strains Isolated from Odiel Salterns (Southwest Spain)Patricia Gómez-Villegas0Javier Vigara1Marta Vila2João Varela3Luísa Barreira4Rosa Léon5Laboratory of Biochemistry, Department of Chemistry, University of Huelva, Avda. de las Fuerzas Armadas s/n, 21071 Huelva, SpainLaboratory of Biochemistry, Department of Chemistry, University of Huelva, Avda. de las Fuerzas Armadas s/n, 21071 Huelva, SpainLaboratory of Biochemistry, Department of Chemistry, University of Huelva, Avda. de las Fuerzas Armadas s/n, 21071 Huelva, SpainCentre of Marine Sciences, University of Algarve, Campus of Gambelas, 8005-139 Faro, PortugalCentre of Marine Sciences, University of Algarve, Campus of Gambelas, 8005-139 Faro, PortugalLaboratory of Biochemistry, Department of Chemistry, University of Huelva, Avda. de las Fuerzas Armadas s/n, 21071 Huelva, SpainThe need to survive in extreme environments has furnished haloarchaea with a series of components specially adapted to work in such conditions. The possible application of these molecules in the pharmaceutical and industrial fields has received increasing attention; however, many potential bioactivities of haloarchaea are still poorly explored. In this paper, we describe the isolation and identification of two new haloarchaeal strains from the saltern ponds located in the marshlands of the Odiel River, in the southwest of Spain, as well as the in vitro assessment of their antioxidant, antimicrobial, and bioactive properties. The acetone extract obtained from the new isolated <i>Haloarcula</i> strain exhibited the highest antioxidant activity, while the acetone extracts from both isolated strains demonstrated a strong antimicrobial activity, especially against other halophilic microorganisms. Moreover, these extracts showed a remarkable ability to inhibit the enzyme cyclooxygenase-2 and to activate the melanogenic enzyme tyrosinase, indicating their potential against chronic inflammation and skin pigmentation disorders. Finally, the aqueous protein-rich extracts obtained from both haloarchaea exhibited an important inhibitory effect on the activity of the acetylcholinesterase enzyme, involved in the hydrolysis of cholinergic neurotransmitters and related to several neurological diseases.https://www.mdpi.com/2079-7737/9/9/298antioxidantanti-inflammatoryantimicrobialbioactive substanceshaloarchaea |
| spellingShingle | Patricia Gómez-Villegas Javier Vigara Marta Vila João Varela Luísa Barreira Rosa Léon Antioxidant, Antimicrobial, and Bioactive Potential of Two New Haloarchaeal Strains Isolated from Odiel Salterns (Southwest Spain) Biology antioxidant anti-inflammatory antimicrobial bioactive substances haloarchaea |
| title | Antioxidant, Antimicrobial, and Bioactive Potential of Two New Haloarchaeal Strains Isolated from Odiel Salterns (Southwest Spain) |
| title_full | Antioxidant, Antimicrobial, and Bioactive Potential of Two New Haloarchaeal Strains Isolated from Odiel Salterns (Southwest Spain) |
| title_fullStr | Antioxidant, Antimicrobial, and Bioactive Potential of Two New Haloarchaeal Strains Isolated from Odiel Salterns (Southwest Spain) |
| title_full_unstemmed | Antioxidant, Antimicrobial, and Bioactive Potential of Two New Haloarchaeal Strains Isolated from Odiel Salterns (Southwest Spain) |
| title_short | Antioxidant, Antimicrobial, and Bioactive Potential of Two New Haloarchaeal Strains Isolated from Odiel Salterns (Southwest Spain) |
| title_sort | antioxidant antimicrobial and bioactive potential of two new haloarchaeal strains isolated from odiel salterns southwest spain |
| topic | antioxidant anti-inflammatory antimicrobial bioactive substances haloarchaea |
| url | https://www.mdpi.com/2079-7737/9/9/298 |
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