Identification of Photosynthesis Characteristics and Chlorophyll Metabolism in Leaves of <i>Citrus</i> Cultivar (<i>Harumi</i>) with Varying Degrees of Chlorosis
In autumn and spring, citrus leaves with a <i>Ponkan</i> (<i>Citrus reticulata</i> Blanco cv. Ponkan) genetic background (<i>Harumi</i>, <i>Daya</i>, etc.) are prone to abnormal physiological chlorosis. The effects of different degrees of chlorosis (no...
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/9/8394 |
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| author | Bo Xiong Ling Li Qin Li Huiqiong Mao Lixinyi Wang Yuhui Bie Xin Zeng Ling Liao Xun Wang Honghong Deng Mingfei Zhang Guochao Sun Zhihui Wang |
| author_facet | Bo Xiong Ling Li Qin Li Huiqiong Mao Lixinyi Wang Yuhui Bie Xin Zeng Ling Liao Xun Wang Honghong Deng Mingfei Zhang Guochao Sun Zhihui Wang |
| author_sort | Bo Xiong |
| collection | DOAJ |
| description | In autumn and spring, citrus leaves with a <i>Ponkan</i> (<i>Citrus reticulata</i> Blanco cv. Ponkan) genetic background (<i>Harumi</i>, <i>Daya</i>, etc.) are prone to abnormal physiological chlorosis. The effects of different degrees of chlorosis (normal, mild, moderate and severe) on photosynthesis and the chlorophyll metabolism of leaves of <i>Citrus</i> cultivar (<i>Harumi</i>) were studied via field experiment. Compared with severe chlorotic leaves, the results showed that chlorosis could break leaf metabolism balance, including reduced chlorophyll content, photosynthetic parameters, antioxidant enzyme activity and enzyme activity related to chlorophyll synthesis, increased catalase and decreased enzyme activity. In addition, the content of chlorophyll synthesis precursors showed an overall downward trend expected for uroporphyrinogen III. Furthermore, the relative expression of genes for chlorophyll synthesis (<i>HEMA1</i>, <i>HEME2</i>, <i>HEMG1</i> and <i>CHLH</i>) was down-regulated to some extent and chlorophyll degradation (<i>CAO</i>, <i>CLH</i>, <i>PPH</i>, <i>PAO</i> and <i>SGR</i>) showed the opposite trend with increased chlorosis. Changes in degradation were more significant. In general, the chlorosis of <i>Harumi</i> leaves might be related to the blocked transformation of uroporphyrinogen III (Urogen III) to coproporphyrinogen III (Coprogen III), the weakening of antioxidant enzyme system activity, the weakening of chlorophyll synthesis and the enhancement in degradation. |
| first_indexed | 2024-03-11T04:16:33Z |
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| language | English |
| last_indexed | 2024-03-11T04:16:33Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-cee1b95afcd84e019f9a7b1e409e0af02023-11-17T23:09:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-01249839410.3390/ijms24098394Identification of Photosynthesis Characteristics and Chlorophyll Metabolism in Leaves of <i>Citrus</i> Cultivar (<i>Harumi</i>) with Varying Degrees of ChlorosisBo Xiong0Ling Li1Qin Li2Huiqiong Mao3Lixinyi Wang4Yuhui Bie5Xin Zeng6Ling Liao7Xun Wang8Honghong Deng9Mingfei Zhang10Guochao Sun11Zhihui Wang12College of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaIn autumn and spring, citrus leaves with a <i>Ponkan</i> (<i>Citrus reticulata</i> Blanco cv. Ponkan) genetic background (<i>Harumi</i>, <i>Daya</i>, etc.) are prone to abnormal physiological chlorosis. The effects of different degrees of chlorosis (normal, mild, moderate and severe) on photosynthesis and the chlorophyll metabolism of leaves of <i>Citrus</i> cultivar (<i>Harumi</i>) were studied via field experiment. Compared with severe chlorotic leaves, the results showed that chlorosis could break leaf metabolism balance, including reduced chlorophyll content, photosynthetic parameters, antioxidant enzyme activity and enzyme activity related to chlorophyll synthesis, increased catalase and decreased enzyme activity. In addition, the content of chlorophyll synthesis precursors showed an overall downward trend expected for uroporphyrinogen III. Furthermore, the relative expression of genes for chlorophyll synthesis (<i>HEMA1</i>, <i>HEME2</i>, <i>HEMG1</i> and <i>CHLH</i>) was down-regulated to some extent and chlorophyll degradation (<i>CAO</i>, <i>CLH</i>, <i>PPH</i>, <i>PAO</i> and <i>SGR</i>) showed the opposite trend with increased chlorosis. Changes in degradation were more significant. In general, the chlorosis of <i>Harumi</i> leaves might be related to the blocked transformation of uroporphyrinogen III (Urogen III) to coproporphyrinogen III (Coprogen III), the weakening of antioxidant enzyme system activity, the weakening of chlorophyll synthesis and the enhancement in degradation.https://www.mdpi.com/1422-0067/24/9/8394<i>Citrus</i>chlorosisphotosynthetic characteristicschlorophyll synthesischlorophyll degradation |
| spellingShingle | Bo Xiong Ling Li Qin Li Huiqiong Mao Lixinyi Wang Yuhui Bie Xin Zeng Ling Liao Xun Wang Honghong Deng Mingfei Zhang Guochao Sun Zhihui Wang Identification of Photosynthesis Characteristics and Chlorophyll Metabolism in Leaves of <i>Citrus</i> Cultivar (<i>Harumi</i>) with Varying Degrees of Chlorosis International Journal of Molecular Sciences <i>Citrus</i> chlorosis photosynthetic characteristics chlorophyll synthesis chlorophyll degradation |
| title | Identification of Photosynthesis Characteristics and Chlorophyll Metabolism in Leaves of <i>Citrus</i> Cultivar (<i>Harumi</i>) with Varying Degrees of Chlorosis |
| title_full | Identification of Photosynthesis Characteristics and Chlorophyll Metabolism in Leaves of <i>Citrus</i> Cultivar (<i>Harumi</i>) with Varying Degrees of Chlorosis |
| title_fullStr | Identification of Photosynthesis Characteristics and Chlorophyll Metabolism in Leaves of <i>Citrus</i> Cultivar (<i>Harumi</i>) with Varying Degrees of Chlorosis |
| title_full_unstemmed | Identification of Photosynthesis Characteristics and Chlorophyll Metabolism in Leaves of <i>Citrus</i> Cultivar (<i>Harumi</i>) with Varying Degrees of Chlorosis |
| title_short | Identification of Photosynthesis Characteristics and Chlorophyll Metabolism in Leaves of <i>Citrus</i> Cultivar (<i>Harumi</i>) with Varying Degrees of Chlorosis |
| title_sort | identification of photosynthesis characteristics and chlorophyll metabolism in leaves of i citrus i cultivar i harumi i with varying degrees of chlorosis |
| topic | <i>Citrus</i> chlorosis photosynthetic characteristics chlorophyll synthesis chlorophyll degradation |
| url | https://www.mdpi.com/1422-0067/24/9/8394 |
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