ANALYSIS OF ENDOPLASMIC RETICULUM OF TOBACCO CELLS USING CONFOCAL MICROSCOPY
Image analysis techniques for preprocessing, segmentation and estimation of geometrical characteristics of fiber-like structures from 2-D or 3-D images captured by a confocal microscope are presented. Methods are demonstrated on fiber-like biological structure: endoplasmic reticulum (ER) of tobacco...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Slovenian Society for Stereology and Quantitative Image Analysis
2011-05-01
|
| Series: | Image Analysis and Stereology |
| Subjects: | |
| Online Access: | http://www.ias-iss.org/ojs/IAS/article/view/785 |
| _version_ | 1819143126825041920 |
|---|---|
| author | Barbora Radochová Jiří Janáček Kateřina Schwarzerová Erna Demjénová Zoltán Tomori Petr Karen Lucie Kubínová |
| author_facet | Barbora Radochová Jiří Janáček Kateřina Schwarzerová Erna Demjénová Zoltán Tomori Petr Karen Lucie Kubínová |
| author_sort | Barbora Radochová |
| collection | DOAJ |
| description | Image analysis techniques for preprocessing, segmentation and estimation of geometrical characteristics of fiber-like structures from 2-D or 3-D images captured by a confocal microscope are presented. Methods are demonstrated on fiber-like biological structure: endoplasmic reticulum (ER) of tobacco cells. In the presented analysis of 2-D images of ER before and after the treatment of latrunculin B, ER and ER tubules were segmented and the area density of ER as well as the length density of ER tubules in the cell cortical layer were estimated by automatic image analysis algorithms. Images of 3-D arrangement of ER were reconstructed and rendered by various visualization techniques. |
| first_indexed | 2024-12-22T12:21:17Z |
| format | Article |
| id | doaj.art-cee352f12a9e450aadfdcb54a539e4a5 |
| institution | Directory Open Access Journal |
| issn | 1580-3139 1854-5165 |
| language | English |
| last_indexed | 2024-12-22T12:21:17Z |
| publishDate | 2011-05-01 |
| publisher | Slovenian Society for Stereology and Quantitative Image Analysis |
| record_format | Article |
| series | Image Analysis and Stereology |
| spelling | doaj.art-cee352f12a9e450aadfdcb54a539e4a52022-12-21T18:25:58ZengSlovenian Society for Stereology and Quantitative Image AnalysisImage Analysis and Stereology1580-31391854-51652011-05-0124318118510.5566/ias.v24.p181-185757ANALYSIS OF ENDOPLASMIC RETICULUM OF TOBACCO CELLS USING CONFOCAL MICROSCOPYBarbora RadochováJiří JanáčekKateřina SchwarzerováErna DemjénováZoltán TomoriPetr KarenLucie KubínováImage analysis techniques for preprocessing, segmentation and estimation of geometrical characteristics of fiber-like structures from 2-D or 3-D images captured by a confocal microscope are presented. Methods are demonstrated on fiber-like biological structure: endoplasmic reticulum (ER) of tobacco cells. In the presented analysis of 2-D images of ER before and after the treatment of latrunculin B, ER and ER tubules were segmented and the area density of ER as well as the length density of ER tubules in the cell cortical layer were estimated by automatic image analysis algorithms. Images of 3-D arrangement of ER were reconstructed and rendered by various visualization techniques.http://www.ias-iss.org/ojs/IAS/article/view/785capillariesconfocal microscopyendoplasmic reticulumfibersimage analysislength density |
| spellingShingle | Barbora Radochová Jiří Janáček Kateřina Schwarzerová Erna Demjénová Zoltán Tomori Petr Karen Lucie Kubínová ANALYSIS OF ENDOPLASMIC RETICULUM OF TOBACCO CELLS USING CONFOCAL MICROSCOPY Image Analysis and Stereology capillaries confocal microscopy endoplasmic reticulum fibers image analysis length density |
| title | ANALYSIS OF ENDOPLASMIC RETICULUM OF TOBACCO CELLS USING CONFOCAL MICROSCOPY |
| title_full | ANALYSIS OF ENDOPLASMIC RETICULUM OF TOBACCO CELLS USING CONFOCAL MICROSCOPY |
| title_fullStr | ANALYSIS OF ENDOPLASMIC RETICULUM OF TOBACCO CELLS USING CONFOCAL MICROSCOPY |
| title_full_unstemmed | ANALYSIS OF ENDOPLASMIC RETICULUM OF TOBACCO CELLS USING CONFOCAL MICROSCOPY |
| title_short | ANALYSIS OF ENDOPLASMIC RETICULUM OF TOBACCO CELLS USING CONFOCAL MICROSCOPY |
| title_sort | analysis of endoplasmic reticulum of tobacco cells using confocal microscopy |
| topic | capillaries confocal microscopy endoplasmic reticulum fibers image analysis length density |
| url | http://www.ias-iss.org/ojs/IAS/article/view/785 |
| work_keys_str_mv | AT barboraradochova analysisofendoplasmicreticulumoftobaccocellsusingconfocalmicroscopy AT jirijanacek analysisofendoplasmicreticulumoftobaccocellsusingconfocalmicroscopy AT katerinaschwarzerova analysisofendoplasmicreticulumoftobaccocellsusingconfocalmicroscopy AT ernademjenova analysisofendoplasmicreticulumoftobaccocellsusingconfocalmicroscopy AT zoltantomori analysisofendoplasmicreticulumoftobaccocellsusingconfocalmicroscopy AT petrkaren analysisofendoplasmicreticulumoftobaccocellsusingconfocalmicroscopy AT luciekubinova analysisofendoplasmicreticulumoftobaccocellsusingconfocalmicroscopy |