Reciprocal recurrent selection effects on the genetic structure of tropical maize populations assessed at microsatellite loci
A modified reciprocal recurrent selection (RRS) method, which employed one cycle of high-intensity selection, was applied to two tropical maize (Zea mays L.) populations, BR-105 and BR-106, originating the improved synthetics IG-3 and IG-4, respectively. In the present study the effects of this kind...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2003-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300022 |
| _version_ | 1818581215280627712 |
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| author | Luciana Rossini Pinto Maria Lucia Carneiro Vieira Claudio Lopes de Souza Jr. Anete Pereira de Souza |
| author_facet | Luciana Rossini Pinto Maria Lucia Carneiro Vieira Claudio Lopes de Souza Jr. Anete Pereira de Souza |
| author_sort | Luciana Rossini Pinto |
| collection | DOAJ |
| description | A modified reciprocal recurrent selection (RRS) method, which employed one cycle of high-intensity selection, was applied to two tropical maize (Zea mays L.) populations, BR-105 and BR-106, originating the improved synthetics IG-3 and IG-4, respectively. In the present study the effects of this kind of selection on the genetic structure of these populations and their synthetics were investigated at 30 microsatellite (SSR) loci. A total of 125 alleles were revealed. A reduction in the number of alleles was observed after selection, as well as changes in allele frequencies. In nearly 13% (BR-105) and 7% (BR-106) of the loci evaluated, the changes in allele frequencies were not explained, exclusively due to the effects of genetic drift. The effective population sizes estimated for the synthetics using 30 SSR loci were similar to those theoretically expected after selection. The genetic differentiation (G ST) between the synthetics increased to 77% compared with the original populations. The estimated R ST values, a genetic differentiation measure proper for microsatellite data, were similar to those obtained for G ST. Despite the high level of selection applied, the total gene diversity found in the synthetics allows them to be used in a new RRS cycle. |
| first_indexed | 2024-12-16T07:29:57Z |
| format | Article |
| id | doaj.art-cee3c5390b7d4b19aee1a3696774b5c8 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-16T07:29:57Z |
| publishDate | 2003-01-01 |
| publisher | Sociedade Brasileira de Genética |
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| series | Genetics and Molecular Biology |
| spelling | doaj.art-cee3c5390b7d4b19aee1a3696774b5c82022-12-21T22:39:23ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852003-01-0126335536410.1590/S1415-47572003000300022Reciprocal recurrent selection effects on the genetic structure of tropical maize populations assessed at microsatellite lociLuciana Rossini PintoMaria Lucia Carneiro VieiraClaudio Lopes de Souza Jr.Anete Pereira de SouzaA modified reciprocal recurrent selection (RRS) method, which employed one cycle of high-intensity selection, was applied to two tropical maize (Zea mays L.) populations, BR-105 and BR-106, originating the improved synthetics IG-3 and IG-4, respectively. In the present study the effects of this kind of selection on the genetic structure of these populations and their synthetics were investigated at 30 microsatellite (SSR) loci. A total of 125 alleles were revealed. A reduction in the number of alleles was observed after selection, as well as changes in allele frequencies. In nearly 13% (BR-105) and 7% (BR-106) of the loci evaluated, the changes in allele frequencies were not explained, exclusively due to the effects of genetic drift. The effective population sizes estimated for the synthetics using 30 SSR loci were similar to those theoretically expected after selection. The genetic differentiation (G ST) between the synthetics increased to 77% compared with the original populations. The estimated R ST values, a genetic differentiation measure proper for microsatellite data, were similar to those obtained for G ST. Despite the high level of selection applied, the total gene diversity found in the synthetics allows them to be used in a new RRS cycle.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300022population geneticsplant breedingmolecular markers |
| spellingShingle | Luciana Rossini Pinto Maria Lucia Carneiro Vieira Claudio Lopes de Souza Jr. Anete Pereira de Souza Reciprocal recurrent selection effects on the genetic structure of tropical maize populations assessed at microsatellite loci Genetics and Molecular Biology population genetics plant breeding molecular markers |
| title | Reciprocal recurrent selection effects on the genetic structure of tropical maize populations assessed at microsatellite loci |
| title_full | Reciprocal recurrent selection effects on the genetic structure of tropical maize populations assessed at microsatellite loci |
| title_fullStr | Reciprocal recurrent selection effects on the genetic structure of tropical maize populations assessed at microsatellite loci |
| title_full_unstemmed | Reciprocal recurrent selection effects on the genetic structure of tropical maize populations assessed at microsatellite loci |
| title_short | Reciprocal recurrent selection effects on the genetic structure of tropical maize populations assessed at microsatellite loci |
| title_sort | reciprocal recurrent selection effects on the genetic structure of tropical maize populations assessed at microsatellite loci |
| topic | population genetics plant breeding molecular markers |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300022 |
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