Genetic Profiles of Escherichia coli Isolated from Indonesian Tempeh Based on Enterobacterial Repetitive Intergenic Consensus- Polymerase Chain Reaction (ERIC-PCR)
Tempeh is a famous Indonesian fermented food derived from soybeans inoculated with Rhizopus sp. Tempeh production varies depend on the producers and often conducted in an uncontrolled condition. This condition could lead to the growth of Escherichia coli which is known as bacterial indicators of env...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Microbiology
2015-06-01
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| Series: | Microbiology Indonesia |
| Subjects: | |
| Online Access: | http://journal.permi.or.id/index.php/mionline/article/view/319 |
| _version_ | 1818359104962297856 |
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| author | QURROTA A’YUN ANTONIUS SUWANTO TATI BARUS |
| author_facet | QURROTA A’YUN ANTONIUS SUWANTO TATI BARUS |
| author_sort | QURROTA A’YUN |
| collection | DOAJ |
| description | Tempeh is a famous Indonesian fermented food derived from soybeans inoculated with Rhizopus sp. Tempeh production varies depend on the producers and often conducted in an uncontrolled condition. This condition could lead to the growth of Escherichia coli which is known as bacterial indicators of environmental hygiene. Some strains of E. coli could induce diarrhea, acute gastroenteritis or gastrointestinal tract infections. The aim of this study was to compare genetic diversity of E. coli isolates from tempeh with medical isolates employing ERIC-PCR method. In this study, 63f and 1387r primers were used to amplify 16S rRNA genes, and ERIC 1R and ERIC 2 primers were used for ERIC-PCR analysis. Tempeh samples were obtained from four producers in Bogor. Thirty-three isolates of E. coli were successfully isolated from tempeh samples produced by only two producers, we could not obtain E. coli isolates from the other two producers. In addition, the same tempeh samples could carry different genotypes of E. coli. On the other hand, the same genotypes could be found in different tempeh samples. Based on phylogenetic tree analysis, E. coli from tempeh could be separated from medical isolates. We showed that E. coli isolates derived from tempeh were genetically different from those of medical or pathogenic isolates. |
| first_indexed | 2024-12-13T20:39:36Z |
| format | Article |
| id | doaj.art-cee656b8932d47a6a86180be01ee098f |
| institution | Directory Open Access Journal |
| issn | 1978-3477 2087-8575 |
| language | English |
| last_indexed | 2024-12-13T20:39:36Z |
| publishDate | 2015-06-01 |
| publisher | Indonesian Society for Microbiology |
| record_format | Article |
| series | Microbiology Indonesia |
| spelling | doaj.art-cee656b8932d47a6a86180be01ee098f2022-12-21T23:32:11ZengIndonesian Society for MicrobiologyMicrobiology Indonesia1978-34772087-85752015-06-0192586410.5454/mi.9.2.2Genetic Profiles of Escherichia coli Isolated from Indonesian Tempeh Based on Enterobacterial Repetitive Intergenic Consensus- Polymerase Chain Reaction (ERIC-PCR)QURROTA A’YUN0ANTONIUS SUWANTO1TATI BARUS2Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor, Darmaga Campus, Bogor 16680, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor, Darmaga Campus, Bogor 16680, IndonesiaDepartment of Biology, Faculty of Biotechnology, Universitas Katolik Atma Jaya, Jalan Jenderal Sudirman No 51, Jakarta 12930, IndonesiaTempeh is a famous Indonesian fermented food derived from soybeans inoculated with Rhizopus sp. Tempeh production varies depend on the producers and often conducted in an uncontrolled condition. This condition could lead to the growth of Escherichia coli which is known as bacterial indicators of environmental hygiene. Some strains of E. coli could induce diarrhea, acute gastroenteritis or gastrointestinal tract infections. The aim of this study was to compare genetic diversity of E. coli isolates from tempeh with medical isolates employing ERIC-PCR method. In this study, 63f and 1387r primers were used to amplify 16S rRNA genes, and ERIC 1R and ERIC 2 primers were used for ERIC-PCR analysis. Tempeh samples were obtained from four producers in Bogor. Thirty-three isolates of E. coli were successfully isolated from tempeh samples produced by only two producers, we could not obtain E. coli isolates from the other two producers. In addition, the same tempeh samples could carry different genotypes of E. coli. On the other hand, the same genotypes could be found in different tempeh samples. Based on phylogenetic tree analysis, E. coli from tempeh could be separated from medical isolates. We showed that E. coli isolates derived from tempeh were genetically different from those of medical or pathogenic isolates.http://journal.permi.or.id/index.php/mionline/article/view/319ERIC-PCREscherichia colitempeh |
| spellingShingle | QURROTA A’YUN ANTONIUS SUWANTO TATI BARUS Genetic Profiles of Escherichia coli Isolated from Indonesian Tempeh Based on Enterobacterial Repetitive Intergenic Consensus- Polymerase Chain Reaction (ERIC-PCR) Microbiology Indonesia ERIC-PCR Escherichia coli tempeh |
| title | Genetic Profiles of Escherichia coli Isolated from Indonesian Tempeh Based on Enterobacterial Repetitive Intergenic Consensus- Polymerase Chain Reaction (ERIC-PCR) |
| title_full | Genetic Profiles of Escherichia coli Isolated from Indonesian Tempeh Based on Enterobacterial Repetitive Intergenic Consensus- Polymerase Chain Reaction (ERIC-PCR) |
| title_fullStr | Genetic Profiles of Escherichia coli Isolated from Indonesian Tempeh Based on Enterobacterial Repetitive Intergenic Consensus- Polymerase Chain Reaction (ERIC-PCR) |
| title_full_unstemmed | Genetic Profiles of Escherichia coli Isolated from Indonesian Tempeh Based on Enterobacterial Repetitive Intergenic Consensus- Polymerase Chain Reaction (ERIC-PCR) |
| title_short | Genetic Profiles of Escherichia coli Isolated from Indonesian Tempeh Based on Enterobacterial Repetitive Intergenic Consensus- Polymerase Chain Reaction (ERIC-PCR) |
| title_sort | genetic profiles of escherichia coli isolated from indonesian tempeh based on enterobacterial repetitive intergenic consensus polymerase chain reaction eric pcr |
| topic | ERIC-PCR Escherichia coli tempeh |
| url | http://journal.permi.or.id/index.php/mionline/article/view/319 |
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