Purification and Characterization of Gigantoxin-4, a New Actinoporin from the Sea Anemone Stichodactyla Gigantea
<p>A new Cytolysin, termed as Gigantoxin-4, was isolated from the sea anemone <i>Stichodactyla gigantea </i>and found to be highly homologous with Cytolysin-3 (HMg III) from <i>Heteractis magnifica</i>, RTX-A from <i>Radianthus macrodactylus</i>, and Stichol...
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| Format: | Article |
| Language: | English |
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Ivyspring International Publisher
2011-01-01
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| Series: | International Journal of Biological Sciences |
| Online Access: | http://www.biolsci.org/v07p0729.htm |
| _version_ | 1828416415642681344 |
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| author | Bo Hu, Wei Guo, Liang-hua Wang, Jian-guang Wang, Xiao-yu Liu, Bing-hua Jiao |
| author_facet | Bo Hu, Wei Guo, Liang-hua Wang, Jian-guang Wang, Xiao-yu Liu, Bing-hua Jiao |
| author_sort | Bo Hu, Wei Guo, Liang-hua Wang, Jian-guang Wang, Xiao-yu Liu, Bing-hua Jiao |
| collection | DOAJ |
| description | <p>A new Cytolysin, termed as Gigantoxin-4, was isolated from the sea anemone <i>Stichodactyla gigantea </i>and found to be highly homologous with Cytolysin-3 (HMg III) from <i>Heteractis magnifica</i>, RTX-A from <i>Radianthus macrodactylus</i>, and Sticholysin-1 (St I) and Sticholysin-2 (St II) from <i>Stichodactyla helianthus</i> (homology 82%, 86%, 82% and 86% respectively). Its 20 N-terminal residues were identified and the full-length cDNA sequence was obtained by reverse transcription-polymerase chain reaction (RT-PCR). Multiple sequence alignments with other Cytolysins of the actinoporin family clearly indicated that Gigantoxin-4 belongs to this protein family. SDS-PAGE electrophoresis showed that this new actinoporin had a molecular mass of about 19 kDa, and possessed a high hemolytic activity to human erythrocytes (HA<sub>50</sub>= 40 ng/ml), which was inhibited by pre-incubation with sphingomyelin (SM) or SM-cholesterol mixtures. Our <i>in vivo</i> experiments showed that Gigantoxin-4 had wide toxicity to the rat cardiovascular system and the respiratory system. A concentration of 30μg/kg Gigantoxin-4, i.v. produced a positive inotropic effect on the rat heart although final cardiovascular failure was inevitable, and 60μg/kg Gigantoxin-4 caused respiratory arrest rapidly resulting in rat death. HE staining indicated pathological changes in various organs and tissues after i.v. administration of Gigantoxin-4.</p> |
| first_indexed | 2024-12-10T14:02:15Z |
| format | Article |
| id | doaj.art-cee7c49a9cff404b9c96f591e65e6b2c |
| institution | Directory Open Access Journal |
| issn | 1449-2288 |
| language | English |
| last_indexed | 2024-12-10T14:02:15Z |
| publishDate | 2011-01-01 |
| publisher | Ivyspring International Publisher |
| record_format | Article |
| series | International Journal of Biological Sciences |
| spelling | doaj.art-cee7c49a9cff404b9c96f591e65e6b2c2022-12-22T01:45:45ZengIvyspring International PublisherInternational Journal of Biological Sciences1449-22882011-01-0176729739Purification and Characterization of Gigantoxin-4, a New Actinoporin from the Sea Anemone Stichodactyla GiganteaBo Hu, Wei Guo, Liang-hua Wang, Jian-guang Wang, Xiao-yu Liu, Bing-hua Jiao<p>A new Cytolysin, termed as Gigantoxin-4, was isolated from the sea anemone <i>Stichodactyla gigantea </i>and found to be highly homologous with Cytolysin-3 (HMg III) from <i>Heteractis magnifica</i>, RTX-A from <i>Radianthus macrodactylus</i>, and Sticholysin-1 (St I) and Sticholysin-2 (St II) from <i>Stichodactyla helianthus</i> (homology 82%, 86%, 82% and 86% respectively). Its 20 N-terminal residues were identified and the full-length cDNA sequence was obtained by reverse transcription-polymerase chain reaction (RT-PCR). Multiple sequence alignments with other Cytolysins of the actinoporin family clearly indicated that Gigantoxin-4 belongs to this protein family. SDS-PAGE electrophoresis showed that this new actinoporin had a molecular mass of about 19 kDa, and possessed a high hemolytic activity to human erythrocytes (HA<sub>50</sub>= 40 ng/ml), which was inhibited by pre-incubation with sphingomyelin (SM) or SM-cholesterol mixtures. Our <i>in vivo</i> experiments showed that Gigantoxin-4 had wide toxicity to the rat cardiovascular system and the respiratory system. A concentration of 30μg/kg Gigantoxin-4, i.v. produced a positive inotropic effect on the rat heart although final cardiovascular failure was inevitable, and 60μg/kg Gigantoxin-4 caused respiratory arrest rapidly resulting in rat death. HE staining indicated pathological changes in various organs and tissues after i.v. administration of Gigantoxin-4.</p>http://www.biolsci.org/v07p0729.htm |
| spellingShingle | Bo Hu, Wei Guo, Liang-hua Wang, Jian-guang Wang, Xiao-yu Liu, Bing-hua Jiao Purification and Characterization of Gigantoxin-4, a New Actinoporin from the Sea Anemone Stichodactyla Gigantea International Journal of Biological Sciences |
| title | Purification and Characterization of Gigantoxin-4, a New Actinoporin from the Sea Anemone Stichodactyla Gigantea |
| title_full | Purification and Characterization of Gigantoxin-4, a New Actinoporin from the Sea Anemone Stichodactyla Gigantea |
| title_fullStr | Purification and Characterization of Gigantoxin-4, a New Actinoporin from the Sea Anemone Stichodactyla Gigantea |
| title_full_unstemmed | Purification and Characterization of Gigantoxin-4, a New Actinoporin from the Sea Anemone Stichodactyla Gigantea |
| title_short | Purification and Characterization of Gigantoxin-4, a New Actinoporin from the Sea Anemone Stichodactyla Gigantea |
| title_sort | purification and characterization of gigantoxin 4 a new actinoporin from the sea anemone stichodactyla gigantea |
| url | http://www.biolsci.org/v07p0729.htm |
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