Effects of Two Distinct Psychoactive Microbes, <i>Lacticaseibacillus rhamnosus</i> JB-1 and <i>Limosilactobacillus reuteri</i> 6475, on Circulating and Hippocampal mRNA in Male Mice
Discovery of the microbiota-gut–brain axis has led to proposed microbe-based therapeutic strategies in mental health, including the use of mood-altering bacterial species, termed psychobiotics. However, we still have limited understanding of the key signaling pathways engaged by specific organisms i...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/17/9653 |
| _version_ | 1797495346115379200 |
|---|---|
| author | Sandor Haas-Neill Eiko Iwashita Anna Dvorkin-Gheva Paul Forsythe |
| author_facet | Sandor Haas-Neill Eiko Iwashita Anna Dvorkin-Gheva Paul Forsythe |
| author_sort | Sandor Haas-Neill |
| collection | DOAJ |
| description | Discovery of the microbiota-gut–brain axis has led to proposed microbe-based therapeutic strategies in mental health, including the use of mood-altering bacterial species, termed psychobiotics. However, we still have limited understanding of the key signaling pathways engaged by specific organisms in modulating brain function, and evidence suggests that bacteria with broadly similar neuroactive and immunomodulatory actions can drive different behavioral outcomes. We sought to identify pathways distinguishing two psychoactive bacterial strains that seemingly engage similar gut–brain signaling pathways but have distinct effects on behaviour. We used RNAseq to identify mRNAs differentially expressed in the blood and hippocampus of mice following <i>Lacticaseibacillus rhamnosus</i> JB-1, and <i>Limosilactobacillus reuteri</i> 6475 treatment and performed Gene Set Enrichment Analysis (GSEA) to identify enrichment in pathway activity. <i>L. rhamnosus</i>, but not <i>L. reuteri</i> treatment altered several pathways in the blood and hippocampus, and the <i>rhamnosus</i> could be clearly distinguished based on mRNA profile. In particular, <i>L. rhamnosus</i> treatment modulated the activity of interferon signaling, JAK/STAT, and TNF-alpha via NF-KB pathways. Our results highlight that psychobiotics can induce complex changes in host gene expression, andin understanding these changes, we may help fine-tune selection of psychobiotics for treating mood disorders. |
| first_indexed | 2024-03-10T01:46:33Z |
| format | Article |
| id | doaj.art-cee8837c1d72458fb56084ca40809b7a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:46:33Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-cee8837c1d72458fb56084ca40809b7a2023-11-23T13:14:28ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012317965310.3390/ijms23179653Effects of Two Distinct Psychoactive Microbes, <i>Lacticaseibacillus rhamnosus</i> JB-1 and <i>Limosilactobacillus reuteri</i> 6475, on Circulating and Hippocampal mRNA in Male MiceSandor Haas-Neill0Eiko Iwashita1Anna Dvorkin-Gheva2Paul Forsythe3The Brain Body Institute, St. Joseph’s Hospital, McMaster University, Hamilton, ON L8N 4A6, CanadaThe Brain Body Institute, St. Joseph’s Hospital, McMaster University, Hamilton, ON L8N 4A6, CanadaMcMaster Immunology Research Centre, Department of Medicine, McMaster University, Hamilton, ON L8N 3Z5, CanadaDivision of Pulmonary Medicine, Department of Medicine, University of Alberta, 569 Heritage Medical Research Center, Edmonton, AB T6G 2S2, CanadaDiscovery of the microbiota-gut–brain axis has led to proposed microbe-based therapeutic strategies in mental health, including the use of mood-altering bacterial species, termed psychobiotics. However, we still have limited understanding of the key signaling pathways engaged by specific organisms in modulating brain function, and evidence suggests that bacteria with broadly similar neuroactive and immunomodulatory actions can drive different behavioral outcomes. We sought to identify pathways distinguishing two psychoactive bacterial strains that seemingly engage similar gut–brain signaling pathways but have distinct effects on behaviour. We used RNAseq to identify mRNAs differentially expressed in the blood and hippocampus of mice following <i>Lacticaseibacillus rhamnosus</i> JB-1, and <i>Limosilactobacillus reuteri</i> 6475 treatment and performed Gene Set Enrichment Analysis (GSEA) to identify enrichment in pathway activity. <i>L. rhamnosus</i>, but not <i>L. reuteri</i> treatment altered several pathways in the blood and hippocampus, and the <i>rhamnosus</i> could be clearly distinguished based on mRNA profile. In particular, <i>L. rhamnosus</i> treatment modulated the activity of interferon signaling, JAK/STAT, and TNF-alpha via NF-KB pathways. Our results highlight that psychobiotics can induce complex changes in host gene expression, andin understanding these changes, we may help fine-tune selection of psychobiotics for treating mood disorders.https://www.mdpi.com/1422-0067/23/17/9653depressionstressgut–brain-axisJB-1psychobioticsmiRNA |
| spellingShingle | Sandor Haas-Neill Eiko Iwashita Anna Dvorkin-Gheva Paul Forsythe Effects of Two Distinct Psychoactive Microbes, <i>Lacticaseibacillus rhamnosus</i> JB-1 and <i>Limosilactobacillus reuteri</i> 6475, on Circulating and Hippocampal mRNA in Male Mice International Journal of Molecular Sciences depression stress gut–brain-axis JB-1 psychobiotics miRNA |
| title | Effects of Two Distinct Psychoactive Microbes, <i>Lacticaseibacillus rhamnosus</i> JB-1 and <i>Limosilactobacillus reuteri</i> 6475, on Circulating and Hippocampal mRNA in Male Mice |
| title_full | Effects of Two Distinct Psychoactive Microbes, <i>Lacticaseibacillus rhamnosus</i> JB-1 and <i>Limosilactobacillus reuteri</i> 6475, on Circulating and Hippocampal mRNA in Male Mice |
| title_fullStr | Effects of Two Distinct Psychoactive Microbes, <i>Lacticaseibacillus rhamnosus</i> JB-1 and <i>Limosilactobacillus reuteri</i> 6475, on Circulating and Hippocampal mRNA in Male Mice |
| title_full_unstemmed | Effects of Two Distinct Psychoactive Microbes, <i>Lacticaseibacillus rhamnosus</i> JB-1 and <i>Limosilactobacillus reuteri</i> 6475, on Circulating and Hippocampal mRNA in Male Mice |
| title_short | Effects of Two Distinct Psychoactive Microbes, <i>Lacticaseibacillus rhamnosus</i> JB-1 and <i>Limosilactobacillus reuteri</i> 6475, on Circulating and Hippocampal mRNA in Male Mice |
| title_sort | effects of two distinct psychoactive microbes i lacticaseibacillus rhamnosus i jb 1 and i limosilactobacillus reuteri i 6475 on circulating and hippocampal mrna in male mice |
| topic | depression stress gut–brain-axis JB-1 psychobiotics miRNA |
| url | https://www.mdpi.com/1422-0067/23/17/9653 |
| work_keys_str_mv | AT sandorhaasneill effectsoftwodistinctpsychoactivemicrobesilacticaseibacillusrhamnosusijb1andilimosilactobacillusreuterii6475oncirculatingandhippocampalmrnainmalemice AT eikoiwashita effectsoftwodistinctpsychoactivemicrobesilacticaseibacillusrhamnosusijb1andilimosilactobacillusreuterii6475oncirculatingandhippocampalmrnainmalemice AT annadvorkingheva effectsoftwodistinctpsychoactivemicrobesilacticaseibacillusrhamnosusijb1andilimosilactobacillusreuterii6475oncirculatingandhippocampalmrnainmalemice AT paulforsythe effectsoftwodistinctpsychoactivemicrobesilacticaseibacillusrhamnosusijb1andilimosilactobacillusreuterii6475oncirculatingandhippocampalmrnainmalemice |