Single Nucleotide Polymorphism Analysis Indicates Genetic Distinction and Reduced Diversity of Swine-Associated Methicillin Resistant Staphylococcus aureus (MRSA) ST5 Isolates Compared to Clinical MRSA ST5 Isolates
Livestock associated methicillin resistant S. aureus (LA-MRSA) are lineages adapted to livestock species. LA-MRSA can be transmitted to humans and public health concerns exist because livestock may be the largest MRSA reservoir outside of hospital settings. Although the predominant European (ST398)...
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Frontiers Media S.A.
2018-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02078/full |
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| author | Samantha J. Hau Anna Allué-Guardia Anna Allué-Guardia Brigida Rusconi Brigida Rusconi Jisun S. Haan Peter R. Davies Timothy S. Frana Mark Eppinger Mark Eppinger Tracy L. Nicholson |
| author_facet | Samantha J. Hau Anna Allué-Guardia Anna Allué-Guardia Brigida Rusconi Brigida Rusconi Jisun S. Haan Peter R. Davies Timothy S. Frana Mark Eppinger Mark Eppinger Tracy L. Nicholson |
| author_sort | Samantha J. Hau |
| collection | DOAJ |
| description | Livestock associated methicillin resistant S. aureus (LA-MRSA) are lineages adapted to livestock species. LA-MRSA can be transmitted to humans and public health concerns exist because livestock may be the largest MRSA reservoir outside of hospital settings. Although the predominant European (ST398) and Asian (ST9) lineages of LA-MRSA are considered livestock adapted, North American swine also harbor ST5, a globally disseminated and highly pathogenic lineage. This study applied whole genome sequencing and single nucleotide polymorphism (SNP) typing to compare the population structure and genetic relatedness between swine associated and human clinical MRSA ST5 isolates. The established high-resolution phylogenomic framework revealed that LA-MRSA and human clinical MRSA ST5 are genetically distinct. LA-MRSA isolates were found to be clonal within farms, while greater genome diversity was observed among sampled clinical MRSA ST5. Analysis of the accessory genome demonstrated that LA-MRSA ST5 isolates and clinical MRSA ST5 isolates harbor different AMR genes and virulence factors, consistent with the SNP analysis. Collectively, our data indicate LA-MRSA and clinical MRSA ST5 isolates are distinct and the swine reservoir is likely of minimal significance as a source of clinical MRSA ST5 infections. |
| first_indexed | 2024-12-13T14:04:08Z |
| format | Article |
| id | doaj.art-cee95f8c42b04620ae14bdcbded54904 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T14:04:08Z |
| publishDate | 2018-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-cee95f8c42b04620ae14bdcbded549042022-12-21T23:42:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-09-01910.3389/fmicb.2018.02078393306Single Nucleotide Polymorphism Analysis Indicates Genetic Distinction and Reduced Diversity of Swine-Associated Methicillin Resistant Staphylococcus aureus (MRSA) ST5 Isolates Compared to Clinical MRSA ST5 IsolatesSamantha J. Hau0Anna Allué-Guardia1Anna Allué-Guardia2Brigida Rusconi3Brigida Rusconi4Jisun S. Haan5Peter R. Davies6Timothy S. Frana7Mark Eppinger8Mark Eppinger9Tracy L. Nicholson10Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, United StatesSouth Texas Center for Emerging Infectious Diseases, University of Texas at San Antonio, San Antonio, TX, United StatesDepartment of Biology, University of Texas at San Antonio, San Antonio, TX, United StatesSouth Texas Center for Emerging Infectious Diseases, University of Texas at San Antonio, San Antonio, TX, United StatesDepartment of Biology, University of Texas at San Antonio, San Antonio, TX, United StatesDepartment of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, United StatesDepartment of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, United StatesDepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, United StatesSouth Texas Center for Emerging Infectious Diseases, University of Texas at San Antonio, San Antonio, TX, United StatesDepartment of Biology, University of Texas at San Antonio, San Antonio, TX, United StatesNational Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, IA, United StatesLivestock associated methicillin resistant S. aureus (LA-MRSA) are lineages adapted to livestock species. LA-MRSA can be transmitted to humans and public health concerns exist because livestock may be the largest MRSA reservoir outside of hospital settings. Although the predominant European (ST398) and Asian (ST9) lineages of LA-MRSA are considered livestock adapted, North American swine also harbor ST5, a globally disseminated and highly pathogenic lineage. This study applied whole genome sequencing and single nucleotide polymorphism (SNP) typing to compare the population structure and genetic relatedness between swine associated and human clinical MRSA ST5 isolates. The established high-resolution phylogenomic framework revealed that LA-MRSA and human clinical MRSA ST5 are genetically distinct. LA-MRSA isolates were found to be clonal within farms, while greater genome diversity was observed among sampled clinical MRSA ST5. Analysis of the accessory genome demonstrated that LA-MRSA ST5 isolates and clinical MRSA ST5 isolates harbor different AMR genes and virulence factors, consistent with the SNP analysis. Collectively, our data indicate LA-MRSA and clinical MRSA ST5 isolates are distinct and the swine reservoir is likely of minimal significance as a source of clinical MRSA ST5 infections.https://www.frontiersin.org/article/10.3389/fmicb.2018.02078/fullLA-MRSAStaphylococcus aureuswhole genome sequence (WGS)single nucleotide polymorphism (SNP) typingphylogenetic analysisswine |
| spellingShingle | Samantha J. Hau Anna Allué-Guardia Anna Allué-Guardia Brigida Rusconi Brigida Rusconi Jisun S. Haan Peter R. Davies Timothy S. Frana Mark Eppinger Mark Eppinger Tracy L. Nicholson Single Nucleotide Polymorphism Analysis Indicates Genetic Distinction and Reduced Diversity of Swine-Associated Methicillin Resistant Staphylococcus aureus (MRSA) ST5 Isolates Compared to Clinical MRSA ST5 Isolates Frontiers in Microbiology LA-MRSA Staphylococcus aureus whole genome sequence (WGS) single nucleotide polymorphism (SNP) typing phylogenetic analysis swine |
| title | Single Nucleotide Polymorphism Analysis Indicates Genetic Distinction and Reduced Diversity of Swine-Associated Methicillin Resistant Staphylococcus aureus (MRSA) ST5 Isolates Compared to Clinical MRSA ST5 Isolates |
| title_full | Single Nucleotide Polymorphism Analysis Indicates Genetic Distinction and Reduced Diversity of Swine-Associated Methicillin Resistant Staphylococcus aureus (MRSA) ST5 Isolates Compared to Clinical MRSA ST5 Isolates |
| title_fullStr | Single Nucleotide Polymorphism Analysis Indicates Genetic Distinction and Reduced Diversity of Swine-Associated Methicillin Resistant Staphylococcus aureus (MRSA) ST5 Isolates Compared to Clinical MRSA ST5 Isolates |
| title_full_unstemmed | Single Nucleotide Polymorphism Analysis Indicates Genetic Distinction and Reduced Diversity of Swine-Associated Methicillin Resistant Staphylococcus aureus (MRSA) ST5 Isolates Compared to Clinical MRSA ST5 Isolates |
| title_short | Single Nucleotide Polymorphism Analysis Indicates Genetic Distinction and Reduced Diversity of Swine-Associated Methicillin Resistant Staphylococcus aureus (MRSA) ST5 Isolates Compared to Clinical MRSA ST5 Isolates |
| title_sort | single nucleotide polymorphism analysis indicates genetic distinction and reduced diversity of swine associated methicillin resistant staphylococcus aureus mrsa st5 isolates compared to clinical mrsa st5 isolates |
| topic | LA-MRSA Staphylococcus aureus whole genome sequence (WGS) single nucleotide polymorphism (SNP) typing phylogenetic analysis swine |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02078/full |
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