Polysaccharides Produced by Plant Growth-Promoting Rhizobacteria Strain <i>Burkholderia</i> sp. BK01 Enhance Salt Stress Tolerance to <i>Arabidopsis thaliana</i>
Salt stress is one of the most serious abiotic stresses leading to reduced agricultural productivity. Polysaccharides from seaweed have been used as biostimulants to promote crop growth and improve plant resistance to abiotic stress. In this study, PGPR strain <i>Burkholderia</i> sp. BK0...
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MDPI AG
2024-01-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/16/1/145 |
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| author | Enni Chen Changsheng Yang Weiyi Tao Shuang Li |
| author_facet | Enni Chen Changsheng Yang Weiyi Tao Shuang Li |
| author_sort | Enni Chen |
| collection | DOAJ |
| description | Salt stress is one of the most serious abiotic stresses leading to reduced agricultural productivity. Polysaccharides from seaweed have been used as biostimulants to promote crop growth and improve plant resistance to abiotic stress. In this study, PGPR strain <i>Burkholderia</i> sp. BK01 was isolated from the rhizosphere of wheat, and it was characterized for phosphorus (Pi) dissolution, indole-3-acetic acid (IAA) production, ammonia (NH<sub>3</sub>) and exopolysaccharides (EPS). In particular, strain BK01 can efficiently produce extracellular polysaccharide with a yield of 12.86 g/L, using sorbitol as carbon source. BK01 EPS was identified as an heteropolysaccharide with Mw 3.559 × 10<sup>6</sup> Da, composed of (D)-galactose (75.3%), (D)-glucose (5.5%), (L)-rhamnose (5.5%), (D)-galactouronic acid (4.9%) and (D)-glucuronic acid (8.8%). The present work aims to highlight the effect of the BK01 EPS on growth and biochemical changes in <i>Arabidopsis thaliana</i> under salt stress (100 mM). The purified BK01 EPS at a concentration of 100 mg/L efficiently promoted the growth of plants in pot assays, improved the chlorophyll content, enhanced the activities of SOD, POD and CAT, and decreased the content of MDA. This results suggested that the polysaccharides produced by PGPR strain <i>Burkholderia</i> sp. BK01 can be used as biostimulants to promote plant growth and improve plant resistance to salt stress. |
| first_indexed | 2024-03-08T14:59:44Z |
| format | Article |
| id | doaj.art-cee96c25cbc143708f8453a14908917a |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-08T14:59:44Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-cee96c25cbc143708f8453a14908917a2024-01-10T15:06:58ZengMDPI AGPolymers2073-43602024-01-0116114510.3390/polym16010145Polysaccharides Produced by Plant Growth-Promoting Rhizobacteria Strain <i>Burkholderia</i> sp. BK01 Enhance Salt Stress Tolerance to <i>Arabidopsis thaliana</i>Enni Chen0Changsheng Yang1Weiyi Tao2Shuang Li3College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, ChinaCollege of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, ChinaCollege of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, ChinaCollege of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, ChinaSalt stress is one of the most serious abiotic stresses leading to reduced agricultural productivity. Polysaccharides from seaweed have been used as biostimulants to promote crop growth and improve plant resistance to abiotic stress. In this study, PGPR strain <i>Burkholderia</i> sp. BK01 was isolated from the rhizosphere of wheat, and it was characterized for phosphorus (Pi) dissolution, indole-3-acetic acid (IAA) production, ammonia (NH<sub>3</sub>) and exopolysaccharides (EPS). In particular, strain BK01 can efficiently produce extracellular polysaccharide with a yield of 12.86 g/L, using sorbitol as carbon source. BK01 EPS was identified as an heteropolysaccharide with Mw 3.559 × 10<sup>6</sup> Da, composed of (D)-galactose (75.3%), (D)-glucose (5.5%), (L)-rhamnose (5.5%), (D)-galactouronic acid (4.9%) and (D)-glucuronic acid (8.8%). The present work aims to highlight the effect of the BK01 EPS on growth and biochemical changes in <i>Arabidopsis thaliana</i> under salt stress (100 mM). The purified BK01 EPS at a concentration of 100 mg/L efficiently promoted the growth of plants in pot assays, improved the chlorophyll content, enhanced the activities of SOD, POD and CAT, and decreased the content of MDA. This results suggested that the polysaccharides produced by PGPR strain <i>Burkholderia</i> sp. BK01 can be used as biostimulants to promote plant growth and improve plant resistance to salt stress.https://www.mdpi.com/2073-4360/16/1/145polysaccharide<i>Burkholderia</i> sp.<i>Arabidopsis thaliana</i>biostimulantssalt stressPGPR |
| spellingShingle | Enni Chen Changsheng Yang Weiyi Tao Shuang Li Polysaccharides Produced by Plant Growth-Promoting Rhizobacteria Strain <i>Burkholderia</i> sp. BK01 Enhance Salt Stress Tolerance to <i>Arabidopsis thaliana</i> Polymers polysaccharide <i>Burkholderia</i> sp. <i>Arabidopsis thaliana</i> biostimulants salt stress PGPR |
| title | Polysaccharides Produced by Plant Growth-Promoting Rhizobacteria Strain <i>Burkholderia</i> sp. BK01 Enhance Salt Stress Tolerance to <i>Arabidopsis thaliana</i> |
| title_full | Polysaccharides Produced by Plant Growth-Promoting Rhizobacteria Strain <i>Burkholderia</i> sp. BK01 Enhance Salt Stress Tolerance to <i>Arabidopsis thaliana</i> |
| title_fullStr | Polysaccharides Produced by Plant Growth-Promoting Rhizobacteria Strain <i>Burkholderia</i> sp. BK01 Enhance Salt Stress Tolerance to <i>Arabidopsis thaliana</i> |
| title_full_unstemmed | Polysaccharides Produced by Plant Growth-Promoting Rhizobacteria Strain <i>Burkholderia</i> sp. BK01 Enhance Salt Stress Tolerance to <i>Arabidopsis thaliana</i> |
| title_short | Polysaccharides Produced by Plant Growth-Promoting Rhizobacteria Strain <i>Burkholderia</i> sp. BK01 Enhance Salt Stress Tolerance to <i>Arabidopsis thaliana</i> |
| title_sort | polysaccharides produced by plant growth promoting rhizobacteria strain i burkholderia i sp bk01 enhance salt stress tolerance to i arabidopsis thaliana i |
| topic | polysaccharide <i>Burkholderia</i> sp. <i>Arabidopsis thaliana</i> biostimulants salt stress PGPR |
| url | https://www.mdpi.com/2073-4360/16/1/145 |
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