Virome of Grapevine Germplasm from the Anapa Ampelographic Collection (Russia)
Grapevine germplasm collections are unique repositories of grape cultivars; therefore, it is necessary to minimize their infection with pathogens, including viruses, and develop various programs to maintain them in a virus-free state. In our study, we examined the virome of the largest Russian grape...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/6/1314 |
| _version_ | 1827655871444811776 |
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| author | Darya Shvets Elena Porotikova Kirill Sandomirsky Svetlana Vinogradova |
| author_facet | Darya Shvets Elena Porotikova Kirill Sandomirsky Svetlana Vinogradova |
| author_sort | Darya Shvets |
| collection | DOAJ |
| description | Grapevine germplasm collections are unique repositories of grape cultivars; therefore, it is necessary to minimize their infection with pathogens, including viruses, and develop various programs to maintain them in a virus-free state. In our study, we examined the virome of the largest Russian grapevine germplasm collection, the Anapa Ampelographic Collection, using high-throughput sequencing of total RNAs. As a result of bioinformatics analysis and validation of its results by reverse transcription PCR (RT-PCR) and quantitative RT-PCR (RT-qPCR), we identified 20 viruses and 3 viroids in 47 libraries. All samples were infected with 2 to 12 viruses and viroids, including those that cause economically significant diseases: leafroll, fleck, and rugose wood complex. For the first time in Russia, we detected Grapevine virus B (GVB), Grapevine virus F (GVF), Grapevine asteroid mosaic-associated virus (GAMaV), Grapevine Red Globe virus (GRGV), Grapevine satellite virus (GV-Sat), Grapevine virga-like virus (GVLV), Grapevine-associated jivivirus 1 (GaJV-1) and Vitis cryptic virus (VCV). A new putative representative of the genus <i>Umbravirus</i> with the provisional name Grapevine umbra-like virus (GULV) was also identified in Russian grape samples. |
| first_indexed | 2024-03-09T22:13:43Z |
| format | Article |
| id | doaj.art-ceea755ded8e483d8d19c002318e6f1f |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T22:13:43Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-ceea755ded8e483d8d19c002318e6f1f2023-11-23T19:27:16ZengMDPI AGViruses1999-49152022-06-01146131410.3390/v14061314Virome of Grapevine Germplasm from the Anapa Ampelographic Collection (Russia)Darya Shvets0Elena Porotikova1Kirill Sandomirsky2Svetlana Vinogradova3Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky, Prospect 33, 119071 Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky, Prospect 33, 119071 Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky, Prospect 33, 119071 Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky, Prospect 33, 119071 Moscow, RussiaGrapevine germplasm collections are unique repositories of grape cultivars; therefore, it is necessary to minimize their infection with pathogens, including viruses, and develop various programs to maintain them in a virus-free state. In our study, we examined the virome of the largest Russian grapevine germplasm collection, the Anapa Ampelographic Collection, using high-throughput sequencing of total RNAs. As a result of bioinformatics analysis and validation of its results by reverse transcription PCR (RT-PCR) and quantitative RT-PCR (RT-qPCR), we identified 20 viruses and 3 viroids in 47 libraries. All samples were infected with 2 to 12 viruses and viroids, including those that cause economically significant diseases: leafroll, fleck, and rugose wood complex. For the first time in Russia, we detected Grapevine virus B (GVB), Grapevine virus F (GVF), Grapevine asteroid mosaic-associated virus (GAMaV), Grapevine Red Globe virus (GRGV), Grapevine satellite virus (GV-Sat), Grapevine virga-like virus (GVLV), Grapevine-associated jivivirus 1 (GaJV-1) and Vitis cryptic virus (VCV). A new putative representative of the genus <i>Umbravirus</i> with the provisional name Grapevine umbra-like virus (GULV) was also identified in Russian grape samples.https://www.mdpi.com/1999-4915/14/6/1314high-throughput sequencingtotal RNAviromegrapevinegermplasmAnapa Ampelographic Collection |
| spellingShingle | Darya Shvets Elena Porotikova Kirill Sandomirsky Svetlana Vinogradova Virome of Grapevine Germplasm from the Anapa Ampelographic Collection (Russia) Viruses high-throughput sequencing total RNA virome grapevine germplasm Anapa Ampelographic Collection |
| title | Virome of Grapevine Germplasm from the Anapa Ampelographic Collection (Russia) |
| title_full | Virome of Grapevine Germplasm from the Anapa Ampelographic Collection (Russia) |
| title_fullStr | Virome of Grapevine Germplasm from the Anapa Ampelographic Collection (Russia) |
| title_full_unstemmed | Virome of Grapevine Germplasm from the Anapa Ampelographic Collection (Russia) |
| title_short | Virome of Grapevine Germplasm from the Anapa Ampelographic Collection (Russia) |
| title_sort | virome of grapevine germplasm from the anapa ampelographic collection russia |
| topic | high-throughput sequencing total RNA virome grapevine germplasm Anapa Ampelographic Collection |
| url | https://www.mdpi.com/1999-4915/14/6/1314 |
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