Horsetail (<i>Equisetum hyemale</i>) Extract Accelerates Wound Healing in Diabetic Rats by Modulating IL-10 and MCP-1 Release and Collagen Synthesis
Traditionally, <i>Equisetum hyemale</i> has been used for wound healing. However, its mechanism of action remains to be elucidated. For this purpose, a 40% ethanolic extract of <i>E. hyemale</i> was prepared. Phytochemical screening revealed the presence of minerals, sterols,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/16/4/514 |
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| author | Hilda Aguayo-Morales Crystel A. Sierra-Rivera Jesús A. Claudio-Rizo Luis E. Cobos-Puc |
| author_facet | Hilda Aguayo-Morales Crystel A. Sierra-Rivera Jesús A. Claudio-Rizo Luis E. Cobos-Puc |
| author_sort | Hilda Aguayo-Morales |
| collection | DOAJ |
| description | Traditionally, <i>Equisetum hyemale</i> has been used for wound healing. However, its mechanism of action remains to be elucidated. For this purpose, a 40% ethanolic extract of <i>E. hyemale</i> was prepared. Phytochemical screening revealed the presence of minerals, sterols, phenolic acids, flavonols, a lignan, and a phenylpropenoid. The extract reduced the viability of RAW 264.7 cells and skin fibroblasts at all times evaluated. On the third day of treatment, this reduction was 30–40% and 15–40%, respectively. In contrast, the extract increased the proliferation of skin fibroblasts only after 48 h. In addition, the extract increased IL-10 release and inhibited MCP-1 release. However, the extract did not affect both TGF-β1 and TNF-α released by RAW 264.7 cells. The higher release of IL-10 could be related to the up-/downregulation of inflammatory pathways mediated by the extract components associated with their bioactivity. The extract inhibited the growth of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. Topical application of the extract accelerated wound healing in diabetic rats by increasing fibroblast collagen synthesis. These results suggest that <i>E. hyemale</i> extract has great potential for use in the treatment of wounds thanks to its phytochemical composition that modulates cytokine secretion, collagen synthesis, and bacterial growth. |
| first_indexed | 2024-03-11T04:39:43Z |
| format | Article |
| id | doaj.art-ceeac790ffeb4e86b9161ed08c96240b |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-11T04:39:43Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-ceeac790ffeb4e86b9161ed08c96240b2023-11-17T20:50:01ZengMDPI AGPharmaceuticals1424-82472023-03-0116451410.3390/ph16040514Horsetail (<i>Equisetum hyemale</i>) Extract Accelerates Wound Healing in Diabetic Rats by Modulating IL-10 and MCP-1 Release and Collagen SynthesisHilda Aguayo-Morales0Crystel A. Sierra-Rivera1Jesús A. Claudio-Rizo2Luis E. Cobos-Puc3Facultad de Ciencias Químicas, Unidad Saltillo, Universidad Autónoma de Coahuila, Boulevard Venustiano Carranza S/N Esquina con Ing. José Cárdenas Valdés, República Oriente, Saltillo 25290, MexicoFacultad de Ciencias Químicas, Unidad Saltillo, Universidad Autónoma de Coahuila, Boulevard Venustiano Carranza S/N Esquina con Ing. José Cárdenas Valdés, República Oriente, Saltillo 25290, MexicoFacultad de Ciencias Químicas, Unidad Saltillo, Universidad Autónoma de Coahuila, Boulevard Venustiano Carranza S/N Esquina con Ing. José Cárdenas Valdés, República Oriente, Saltillo 25290, MexicoFacultad de Ciencias Químicas, Unidad Saltillo, Universidad Autónoma de Coahuila, Boulevard Venustiano Carranza S/N Esquina con Ing. José Cárdenas Valdés, República Oriente, Saltillo 25290, MexicoTraditionally, <i>Equisetum hyemale</i> has been used for wound healing. However, its mechanism of action remains to be elucidated. For this purpose, a 40% ethanolic extract of <i>E. hyemale</i> was prepared. Phytochemical screening revealed the presence of minerals, sterols, phenolic acids, flavonols, a lignan, and a phenylpropenoid. The extract reduced the viability of RAW 264.7 cells and skin fibroblasts at all times evaluated. On the third day of treatment, this reduction was 30–40% and 15–40%, respectively. In contrast, the extract increased the proliferation of skin fibroblasts only after 48 h. In addition, the extract increased IL-10 release and inhibited MCP-1 release. However, the extract did not affect both TGF-β1 and TNF-α released by RAW 264.7 cells. The higher release of IL-10 could be related to the up-/downregulation of inflammatory pathways mediated by the extract components associated with their bioactivity. The extract inhibited the growth of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. Topical application of the extract accelerated wound healing in diabetic rats by increasing fibroblast collagen synthesis. These results suggest that <i>E. hyemale</i> extract has great potential for use in the treatment of wounds thanks to its phytochemical composition that modulates cytokine secretion, collagen synthesis, and bacterial growth.https://www.mdpi.com/1424-8247/16/4/514<i>Equisetum hyemale</i>inflammationcytokinesphenolic compoundswound healingtype 2 diabetic rats |
| spellingShingle | Hilda Aguayo-Morales Crystel A. Sierra-Rivera Jesús A. Claudio-Rizo Luis E. Cobos-Puc Horsetail (<i>Equisetum hyemale</i>) Extract Accelerates Wound Healing in Diabetic Rats by Modulating IL-10 and MCP-1 Release and Collagen Synthesis Pharmaceuticals <i>Equisetum hyemale</i> inflammation cytokines phenolic compounds wound healing type 2 diabetic rats |
| title | Horsetail (<i>Equisetum hyemale</i>) Extract Accelerates Wound Healing in Diabetic Rats by Modulating IL-10 and MCP-1 Release and Collagen Synthesis |
| title_full | Horsetail (<i>Equisetum hyemale</i>) Extract Accelerates Wound Healing in Diabetic Rats by Modulating IL-10 and MCP-1 Release and Collagen Synthesis |
| title_fullStr | Horsetail (<i>Equisetum hyemale</i>) Extract Accelerates Wound Healing in Diabetic Rats by Modulating IL-10 and MCP-1 Release and Collagen Synthesis |
| title_full_unstemmed | Horsetail (<i>Equisetum hyemale</i>) Extract Accelerates Wound Healing in Diabetic Rats by Modulating IL-10 and MCP-1 Release and Collagen Synthesis |
| title_short | Horsetail (<i>Equisetum hyemale</i>) Extract Accelerates Wound Healing in Diabetic Rats by Modulating IL-10 and MCP-1 Release and Collagen Synthesis |
| title_sort | horsetail i equisetum hyemale i extract accelerates wound healing in diabetic rats by modulating il 10 and mcp 1 release and collagen synthesis |
| topic | <i>Equisetum hyemale</i> inflammation cytokines phenolic compounds wound healing type 2 diabetic rats |
| url | https://www.mdpi.com/1424-8247/16/4/514 |
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