Down Regulation of Chemerin and Resistin in Type II Diabetic Rats Treated With Silver Nanoparticles Synthetized Using Eryngium thyrsoideum Boiss Extract
Introduction: Diabetes is related to the higher production of inflammatory markers such as chemerin and resistin. Hypoglycemic influences of Eryngium thyrsoideum Boiss and silver nanoparticles (SNPs) have been established. The present study investigated the impacts of chemically or biologically synt...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zabol University of Medical sciences
2021-12-01
|
| Series: | International Journal of Basic Science in Medicine |
| Subjects: | |
| Online Access: | https://ijbsm.zbmu.ac.ir/PDF/ijbsm-6-132.pdf |
| _version_ | 1818048098374516736 |
|---|---|
| author | Samad Poureskandar Souha Fariba Mahmoudi Ezzat Noorizadeh Alireza Panahi |
| author_facet | Samad Poureskandar Souha Fariba Mahmoudi Ezzat Noorizadeh Alireza Panahi |
| author_sort | Samad Poureskandar Souha |
| collection | DOAJ |
| description | Introduction: Diabetes is related to the higher production of inflammatory markers such as chemerin and resistin. Hypoglycemic influences of Eryngium thyrsoideum Boiss and silver nanoparticles (SNPs) have been established. The present study investigated the impacts of chemically or biologically synthesized SNPs using E. thyrsoideum extract on chemerin and resistin gene expressions in type II diabetic rats. Methods: Twenty male Wistar rats weighing 180-200 g were used. Type 2 diabetes was induced by nicotinamide and streptozotocin. Saline and 2.5 mg/kg chemically or biologically synthesized SNPs were injected to diabetic rats for two weeks. Five healthy rats received saline for two weeks. One day after the last injection, fasting blood samples were collected. Mean serum concentrations of glucose, creatinine, urea, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were measured by spectrophotometry. The gene expression of chemerin and resistin was determined by real-time polymerase chain reaction (PCR). Results: Mean serum concentrations of glucose, urea, ALT, AST, and mean relative gene expression of chemerin and resistin remarkably decreased in the diabetic group receiving SNPs prepared by either the chemical method or using Eryngium extract compared to the diabetic control group. Serum creatinine concentration did not change significantly in the control diabetic or diabetic groups receiving SNPs prepared either chemically or using the Eryngium extract. Conclusion: Chemically or green synthesized SNPs prevent liver hepatocyte damage. They reduced inflammatory factors via exerting hypoglycemic effects. |
| first_indexed | 2024-12-10T10:16:17Z |
| format | Article |
| id | doaj.art-ceed44fdcb7242a8855b45b43e7859ba |
| institution | Directory Open Access Journal |
| issn | 2476-664X |
| language | English |
| last_indexed | 2024-12-10T10:16:17Z |
| publishDate | 2021-12-01 |
| publisher | Zabol University of Medical sciences |
| record_format | Article |
| series | International Journal of Basic Science in Medicine |
| spelling | doaj.art-ceed44fdcb7242a8855b45b43e7859ba2022-12-22T01:53:00ZengZabol University of Medical sciencesInternational Journal of Basic Science in Medicine2476-664X2021-12-016413213810.34172/ijbsm.2021.24ijbsm-23528Down Regulation of Chemerin and Resistin in Type II Diabetic Rats Treated With Silver Nanoparticles Synthetized Using Eryngium thyrsoideum Boiss ExtractSamad Poureskandar Souha0Fariba Mahmoudi1Ezzat Noorizadeh2Alireza Panahi3Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, IranFaculty of Sciences, University of Mohaghegh Ardabili, Ardabil, IranFaculty of Sciences, University of Mohaghegh Ardabili, Ardabil, IranFaculty of Sciences, University of Mohaghegh Ardabili, Ardabil, IranIntroduction: Diabetes is related to the higher production of inflammatory markers such as chemerin and resistin. Hypoglycemic influences of Eryngium thyrsoideum Boiss and silver nanoparticles (SNPs) have been established. The present study investigated the impacts of chemically or biologically synthesized SNPs using E. thyrsoideum extract on chemerin and resistin gene expressions in type II diabetic rats. Methods: Twenty male Wistar rats weighing 180-200 g were used. Type 2 diabetes was induced by nicotinamide and streptozotocin. Saline and 2.5 mg/kg chemically or biologically synthesized SNPs were injected to diabetic rats for two weeks. Five healthy rats received saline for two weeks. One day after the last injection, fasting blood samples were collected. Mean serum concentrations of glucose, creatinine, urea, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were measured by spectrophotometry. The gene expression of chemerin and resistin was determined by real-time polymerase chain reaction (PCR). Results: Mean serum concentrations of glucose, urea, ALT, AST, and mean relative gene expression of chemerin and resistin remarkably decreased in the diabetic group receiving SNPs prepared by either the chemical method or using Eryngium extract compared to the diabetic control group. Serum creatinine concentration did not change significantly in the control diabetic or diabetic groups receiving SNPs prepared either chemically or using the Eryngium extract. Conclusion: Chemically or green synthesized SNPs prevent liver hepatocyte damage. They reduced inflammatory factors via exerting hypoglycemic effects.https://ijbsm.zbmu.ac.ir/PDF/ijbsm-6-132.pdferyngium thyrsoideumsilver nanoparticleschemerinresistin |
| spellingShingle | Samad Poureskandar Souha Fariba Mahmoudi Ezzat Noorizadeh Alireza Panahi Down Regulation of Chemerin and Resistin in Type II Diabetic Rats Treated With Silver Nanoparticles Synthetized Using Eryngium thyrsoideum Boiss Extract International Journal of Basic Science in Medicine eryngium thyrsoideum silver nanoparticles chemerin resistin |
| title | Down Regulation of Chemerin and Resistin in Type II Diabetic Rats Treated With Silver Nanoparticles Synthetized Using Eryngium thyrsoideum Boiss Extract |
| title_full | Down Regulation of Chemerin and Resistin in Type II Diabetic Rats Treated With Silver Nanoparticles Synthetized Using Eryngium thyrsoideum Boiss Extract |
| title_fullStr | Down Regulation of Chemerin and Resistin in Type II Diabetic Rats Treated With Silver Nanoparticles Synthetized Using Eryngium thyrsoideum Boiss Extract |
| title_full_unstemmed | Down Regulation of Chemerin and Resistin in Type II Diabetic Rats Treated With Silver Nanoparticles Synthetized Using Eryngium thyrsoideum Boiss Extract |
| title_short | Down Regulation of Chemerin and Resistin in Type II Diabetic Rats Treated With Silver Nanoparticles Synthetized Using Eryngium thyrsoideum Boiss Extract |
| title_sort | down regulation of chemerin and resistin in type ii diabetic rats treated with silver nanoparticles synthetized using eryngium thyrsoideum boiss extract |
| topic | eryngium thyrsoideum silver nanoparticles chemerin resistin |
| url | https://ijbsm.zbmu.ac.ir/PDF/ijbsm-6-132.pdf |
| work_keys_str_mv | AT samadpoureskandarsouha downregulationofchemerinandresistinintypeiidiabeticratstreatedwithsilvernanoparticlessynthetizedusingeryngiumthyrsoideumboissextract AT faribamahmoudi downregulationofchemerinandresistinintypeiidiabeticratstreatedwithsilvernanoparticlessynthetizedusingeryngiumthyrsoideumboissextract AT ezzatnoorizadeh downregulationofchemerinandresistinintypeiidiabeticratstreatedwithsilvernanoparticlessynthetizedusingeryngiumthyrsoideumboissextract AT alirezapanahi downregulationofchemerinandresistinintypeiidiabeticratstreatedwithsilvernanoparticlessynthetizedusingeryngiumthyrsoideumboissextract |