Genotype MTBDRsl version 2 and phenotypic drug resistance detection of Mycobacterium tuberculosis for fluoroquinolones and aminoglycosides
Background: Information on genotypic with comparison of phenotypic drug sensitivity test of anti-tuberculosis (TB) has been reported in several studies, which have variable results. The present study aimed to assess the Genotype MTBDRsl version 2.0/Line probe assay (LPA) for the detection of fluoroq...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | International Journal of Mycobacteriology |
| Subjects: | |
| Online Access: | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2023;volume=12;issue=3;spage=299;epage=304;aulast=Radhakrishnan |
| _version_ | 1797649325661093888 |
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| author | Ramalingam Radhakrishnan S Prabuseenivasan S Hannah V Vaishanavee V Senthildevi T Kannadhasan R Suchithra Priya Rajendran Ponnuraja Chinnaiyan Lavanya Jayabal Asha Frederick Sivakumar Shanmugam |
| author_facet | Ramalingam Radhakrishnan S Prabuseenivasan S Hannah V Vaishanavee V Senthildevi T Kannadhasan R Suchithra Priya Rajendran Ponnuraja Chinnaiyan Lavanya Jayabal Asha Frederick Sivakumar Shanmugam |
| author_sort | Ramalingam Radhakrishnan |
| collection | DOAJ |
| description | Background: Information on genotypic with comparison of phenotypic drug sensitivity test of anti-tuberculosis (TB) has been reported in several studies, which have variable results. The present study aimed to assess the Genotype MTBDRsl version 2.0/Line probe assay (LPA) for the detection of fluoroquinolones (FQ) and aminoglycosides (AMGs) resistance mutations among drug-resistant Mycobacterium TB (MTB) strains and also to compare the patterns of genotypic mutations of gyrA/B, rrs, and eis with mycobacteria growth indicator tube (MGIT 960). Methods: A total of 1416 samples were subjected to Genotype MTBDRsl version 2.0 assay. One hundred and twenty sputum smear positive MTB isolates and 37 sputum smear negative MTB isolates confirmed multiple drug resistance resistant to FQ and AMG by the Genotype MTBDRsl version 2.0 were subjected to phenotypic drug susceptibility testing (DST) were analyzed. Results: The association sensitivity, specificity, positive predictive value, negative predictive value, and accuracy for the resistance detection between MGIT (DST) and the Genotype MTBDRsl version 2.0 assay was significant (P < 0.01) of moxifloxacin (MFX) concentration. Sensitivity and specificity value for kanamycin (KAN) resistance was 76% and 89%; 47% and 94% for capreomycin (CAP); and 60% and 76% for low-level KAN, respectively. Conclusion: Our results indicate that MFX (0.25and 1 μg/mL), KAN (2.5 μg/mL), and CAP (2.5 μg/mL) significantly (P < 0.01) and support the World Health Organization guidance to test FQ and AMG by genotypic test. |
| first_indexed | 2024-03-11T15:44:30Z |
| format | Article |
| id | doaj.art-cef080d590d74addb89b79a63698339d |
| institution | Directory Open Access Journal |
| issn | 2212-5531 2212-554X |
| language | English |
| last_indexed | 2024-03-11T15:44:30Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Mycobacteriology |
| spelling | doaj.art-cef080d590d74addb89b79a63698339d2023-10-26T07:27:42ZengWolters Kluwer Medknow PublicationsInternational Journal of Mycobacteriology2212-55312212-554X2023-01-0112329930410.4103/ijmy.ijmy_120_23Genotype MTBDRsl version 2 and phenotypic drug resistance detection of Mycobacterium tuberculosis for fluoroquinolones and aminoglycosidesRamalingam RadhakrishnanS PrabuseenivasanS HannahV VaishanaveeV SenthildeviT KannadhasanR SuchithraPriya RajendranPonnuraja ChinnaiyanLavanya JayabalAsha FrederickSivakumar ShanmugamBackground: Information on genotypic with comparison of phenotypic drug sensitivity test of anti-tuberculosis (TB) has been reported in several studies, which have variable results. The present study aimed to assess the Genotype MTBDRsl version 2.0/Line probe assay (LPA) for the detection of fluoroquinolones (FQ) and aminoglycosides (AMGs) resistance mutations among drug-resistant Mycobacterium TB (MTB) strains and also to compare the patterns of genotypic mutations of gyrA/B, rrs, and eis with mycobacteria growth indicator tube (MGIT 960). Methods: A total of 1416 samples were subjected to Genotype MTBDRsl version 2.0 assay. One hundred and twenty sputum smear positive MTB isolates and 37 sputum smear negative MTB isolates confirmed multiple drug resistance resistant to FQ and AMG by the Genotype MTBDRsl version 2.0 were subjected to phenotypic drug susceptibility testing (DST) were analyzed. Results: The association sensitivity, specificity, positive predictive value, negative predictive value, and accuracy for the resistance detection between MGIT (DST) and the Genotype MTBDRsl version 2.0 assay was significant (P < 0.01) of moxifloxacin (MFX) concentration. Sensitivity and specificity value for kanamycin (KAN) resistance was 76% and 89%; 47% and 94% for capreomycin (CAP); and 60% and 76% for low-level KAN, respectively. Conclusion: Our results indicate that MFX (0.25and 1 μg/mL), KAN (2.5 μg/mL), and CAP (2.5 μg/mL) significantly (P < 0.01) and support the World Health Organization guidance to test FQ and AMG by genotypic test.http://www.ijmyco.org/article.asp?issn=2212-5531;year=2023;volume=12;issue=3;spage=299;epage=304;aulast=Radhakrishnanaminoglycosidesfluoroquinolonesline probe assay |
| spellingShingle | Ramalingam Radhakrishnan S Prabuseenivasan S Hannah V Vaishanavee V Senthildevi T Kannadhasan R Suchithra Priya Rajendran Ponnuraja Chinnaiyan Lavanya Jayabal Asha Frederick Sivakumar Shanmugam Genotype MTBDRsl version 2 and phenotypic drug resistance detection of Mycobacterium tuberculosis for fluoroquinolones and aminoglycosides International Journal of Mycobacteriology aminoglycosides fluoroquinolones line probe assay |
| title | Genotype MTBDRsl version 2 and phenotypic drug resistance detection of Mycobacterium tuberculosis for fluoroquinolones and aminoglycosides |
| title_full | Genotype MTBDRsl version 2 and phenotypic drug resistance detection of Mycobacterium tuberculosis for fluoroquinolones and aminoglycosides |
| title_fullStr | Genotype MTBDRsl version 2 and phenotypic drug resistance detection of Mycobacterium tuberculosis for fluoroquinolones and aminoglycosides |
| title_full_unstemmed | Genotype MTBDRsl version 2 and phenotypic drug resistance detection of Mycobacterium tuberculosis for fluoroquinolones and aminoglycosides |
| title_short | Genotype MTBDRsl version 2 and phenotypic drug resistance detection of Mycobacterium tuberculosis for fluoroquinolones and aminoglycosides |
| title_sort | genotype mtbdrsl version 2 and phenotypic drug resistance detection of mycobacterium tuberculosis for fluoroquinolones and aminoglycosides |
| topic | aminoglycosides fluoroquinolones line probe assay |
| url | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2023;volume=12;issue=3;spage=299;epage=304;aulast=Radhakrishnan |
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