The association of TP53 (Arg72Pro) rs1042522 C>G polymorphism and colorectal cancer susceptibility in Duhok individuals
Background and aim: The TP53 gene encodes an important class of cell cycle and tumor-suppressing factors that play critical roles in maintaining genomic stability. The TP53 Arg72Pro (rs1042522 C>G) polymorphism has been reported to be associated with the risk of several types of adult cancers. Ob...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Salahaddin University-Erbil
2023-08-01
|
| Series: | Zanco Journal of Pure and Applied Sciences |
| Subjects: | |
| Online Access: | https://zancojournal.su.edu.krd/index.php/JPAS/article/view/647 |
| _version_ | 1797347713791033344 |
|---|---|
| author | Najat Taher Mahmood ,Dilan Jasim Khalil ,Salar Saadi Hussain ,Ibrahim Hasan Mustafa ,Farida Fariq Nerweyi |
| author_facet | Najat Taher Mahmood ,Dilan Jasim Khalil ,Salar Saadi Hussain ,Ibrahim Hasan Mustafa ,Farida Fariq Nerweyi |
| author_sort | Najat Taher Mahmood ,Dilan Jasim Khalil ,Salar Saadi Hussain ,Ibrahim Hasan Mustafa ,Farida Fariq Nerweyi |
| collection | DOAJ |
| description | Background and aim: The TP53 gene encodes an important class of cell cycle and tumor-suppressing factors that play critical roles in maintaining genomic stability. The TP53 Arg72Pro (rs1042522 C>G) polymorphism has been reported to be associated with the risk of several types of adult cancers. Objective of this study was to investigate the possible association between TP53 Arg72Pro polymorphism with colorectal cancer and to examine its correlation with age groups, Tumor type, Nodal state, and Duck stage of individual.
Patients and methods: The study involved 50 patients with colorectal cancer (25 males and 25 females). This study was conducted to estimate the distribution of colorectal cancer within age groups, Tumor type, Nodal state, and Duck stage of individual also to investigate the distribution of TP53 Arg72Pro SNPs genotype in colorectal cancer, and determine whether TP53 Arg72Pro polymorphism is a possible relevance in susceptibility to colorectal cancer using RFLP-PCR analysis.
Results: The present study shows there were no statistically significant differences between the different age groups and Dukes states with gender. Additionally, tumor types and nodal states (either positive or negative) of colorectal cancer were significantly different with gender. Also, this study revealed that 10 (20.0%) colorectal cancer patients had a 152 bp undigested PCR product fragment representing homozygotes for proline, 8 (16.0%) had two 50 and 102 bp fragments representing homozygotes for arginine, and 32 (64.0%) had three 50, 102, and 152 bp fragments representing heterozygotes for proline. The results of control genotypes showed 14 (28.0%) people with a fragment of 152 bp indicating homozygotes proline, 20 (40.0%) with two fragments representing homozygotes arginine, and 14 (28.0%) with three bands showing heterozygotes for codon 72.
Conclusion: No substantial differences (P>0.05) between the frequency of Arg72allele and Pro72allele in colorectal cancer -affected males as opposed to the frequency of Arg72allele and Pro72allele in the control groups. In relation to the frequency of Arg72allele and Pro72allele in females, indicates substantial differences (P>0.05). |
| first_indexed | 2024-03-08T11:51:46Z |
| format | Article |
| id | doaj.art-cef7212c71cf4b99a6218fdeeb9b6dc9 |
| institution | Directory Open Access Journal |
| issn | 2218-0230 2412-3986 |
| language | English |
| last_indexed | 2024-03-08T11:51:46Z |
| publishDate | 2023-08-01 |
| publisher | Salahaddin University-Erbil |
| record_format | Article |
| series | Zanco Journal of Pure and Applied Sciences |
| spelling | doaj.art-cef7212c71cf4b99a6218fdeeb9b6dc92024-01-24T09:28:06ZengSalahaddin University-ErbilZanco Journal of Pure and Applied Sciences2218-02302412-39862023-08-0135410.21271/ZJPAS.35.4.20The association of TP53 (Arg72Pro) rs1042522 C>G polymorphism and colorectal cancer susceptibility in Duhok individualsNajat Taher Mahmood ,Dilan Jasim Khalil ,Salar Saadi Hussain ,Ibrahim Hasan Mustafa ,Farida Fariq NerweyiBackground and aim: The TP53 gene encodes an important class of cell cycle and tumor-suppressing factors that play critical roles in maintaining genomic stability. The TP53 Arg72Pro (rs1042522 C>G) polymorphism has been reported to be associated with the risk of several types of adult cancers. Objective of this study was to investigate the possible association between TP53 Arg72Pro polymorphism with colorectal cancer and to examine its correlation with age groups, Tumor type, Nodal state, and Duck stage of individual. Patients and methods: The study involved 50 patients with colorectal cancer (25 males and 25 females). This study was conducted to estimate the distribution of colorectal cancer within age groups, Tumor type, Nodal state, and Duck stage of individual also to investigate the distribution of TP53 Arg72Pro SNPs genotype in colorectal cancer, and determine whether TP53 Arg72Pro polymorphism is a possible relevance in susceptibility to colorectal cancer using RFLP-PCR analysis. Results: The present study shows there were no statistically significant differences between the different age groups and Dukes states with gender. Additionally, tumor types and nodal states (either positive or negative) of colorectal cancer were significantly different with gender. Also, this study revealed that 10 (20.0%) colorectal cancer patients had a 152 bp undigested PCR product fragment representing homozygotes for proline, 8 (16.0%) had two 50 and 102 bp fragments representing homozygotes for arginine, and 32 (64.0%) had three 50, 102, and 152 bp fragments representing heterozygotes for proline. The results of control genotypes showed 14 (28.0%) people with a fragment of 152 bp indicating homozygotes proline, 20 (40.0%) with two fragments representing homozygotes arginine, and 14 (28.0%) with three bands showing heterozygotes for codon 72. Conclusion: No substantial differences (P>0.05) between the frequency of Arg72allele and Pro72allele in colorectal cancer -affected males as opposed to the frequency of Arg72allele and Pro72allele in the control groups. In relation to the frequency of Arg72allele and Pro72allele in females, indicates substantial differences (P>0.05).https://zancojournal.su.edu.krd/index.php/JPAS/article/view/647tp53 (arg72pro) rs1042522 c>g polymorphismcolorectal cancerduhok |
| spellingShingle | Najat Taher Mahmood ,Dilan Jasim Khalil ,Salar Saadi Hussain ,Ibrahim Hasan Mustafa ,Farida Fariq Nerweyi The association of TP53 (Arg72Pro) rs1042522 C>G polymorphism and colorectal cancer susceptibility in Duhok individuals Zanco Journal of Pure and Applied Sciences tp53 (arg72pro) rs1042522 c>g polymorphism colorectal cancer duhok |
| title | The association of TP53 (Arg72Pro) rs1042522 C>G polymorphism and colorectal cancer susceptibility in Duhok individuals |
| title_full | The association of TP53 (Arg72Pro) rs1042522 C>G polymorphism and colorectal cancer susceptibility in Duhok individuals |
| title_fullStr | The association of TP53 (Arg72Pro) rs1042522 C>G polymorphism and colorectal cancer susceptibility in Duhok individuals |
| title_full_unstemmed | The association of TP53 (Arg72Pro) rs1042522 C>G polymorphism and colorectal cancer susceptibility in Duhok individuals |
| title_short | The association of TP53 (Arg72Pro) rs1042522 C>G polymorphism and colorectal cancer susceptibility in Duhok individuals |
| title_sort | association of tp53 arg72pro rs1042522 c g polymorphism and colorectal cancer susceptibility in duhok individuals |
| topic | tp53 (arg72pro) rs1042522 c>g polymorphism colorectal cancer duhok |
| url | https://zancojournal.su.edu.krd/index.php/JPAS/article/view/647 |
| work_keys_str_mv | AT najattahermahmooddilanjasimkhalilsalarsaadihussainibrahimhasanmustafafaridafariqnerweyi theassociationoftp53arg72prors1042522cgpolymorphismandcolorectalcancersusceptibilityinduhokindividuals AT najattahermahmooddilanjasimkhalilsalarsaadihussainibrahimhasanmustafafaridafariqnerweyi associationoftp53arg72prors1042522cgpolymorphismandcolorectalcancersusceptibilityinduhokindividuals |