Viscoelastic Properties of Acellular Matrices of Porcine Esophageal Mucosa and Comparison with Acellular Matrices of Porcine Small Intestine Submucosa and Bovine Pericardium
The aim of this study was to compare the viscoelastic properties of a decellularized mesh from the porcine esophagus, prepared by our group, with two commercial acellular tissues derived from porcine small intestine submucosa and bovine pericardium for use in medical devices. The tissues’ viscoelast...
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| author | Sergio Estrada Mira María Inmaculada García-Briega José Luis Gómez Ribelles Luz M. Restrepo Munera |
| author_facet | Sergio Estrada Mira María Inmaculada García-Briega José Luis Gómez Ribelles Luz M. Restrepo Munera |
| author_sort | Sergio Estrada Mira |
| collection | DOAJ |
| description | The aim of this study was to compare the viscoelastic properties of a decellularized mesh from the porcine esophagus, prepared by our group, with two commercial acellular tissues derived from porcine small intestine submucosa and bovine pericardium for use in medical devices. The tissues’ viscoelastic properties were characterized by creep tests in tension, applying the load in the direction of the fibers or the transverse direction, and also by dynamic-shear mechanical tests between parallel plates or in tension at frequencies between 0.1 and 35 Hz. All the tests were performed in triplicate at a constant temperature of 37 °C immersed in distilled water. The tissues’ surface and cross-sectional microstructure were observed by scanning electron microscopy (SEM) to characterize the orientation of the fibers. The matrices of the porcine esophagus present an elastic modulus in the order of 60 MPa when loaded in the longitudinal direction while those of the porcine intestine submucosa and bovine pericardium have an elastic modulus below 5 MPa. Nevertheless, the shear modulus of bovine pericardium nearly triplicates that of the esophageal matrix. The viscoelasticity of decellularized esophageal mucosa is characterized by a fast change in the creep compliance with time. The slope of the creep curve in the double logarithmic plot is twice that of the control samples. These results are consistent with the microstructure observed under electron microscopy regarding the orientation of the fibers that make up the matrices. |
| first_indexed | 2024-03-08T15:02:48Z |
| format | Article |
| id | doaj.art-cef85924b464465985fa6d96da0d998b |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-08T15:02:48Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
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| series | Materials |
| spelling | doaj.art-cef85924b464465985fa6d96da0d998b2024-01-10T15:02:43ZengMDPI AGMaterials1996-19442023-12-0117113410.3390/ma17010134Viscoelastic Properties of Acellular Matrices of Porcine Esophageal Mucosa and Comparison with Acellular Matrices of Porcine Small Intestine Submucosa and Bovine PericardiumSergio Estrada Mira0María Inmaculada García-Briega1José Luis Gómez Ribelles2Luz M. Restrepo Munera3Tissue Engineering and Cells Therapy Group (GITTC), School of Medicine, University of Antioquia, Medellin 050010, ColombiaCentre for Biomaterials and Tissue Engineering (CBIT), Universitat Politècnica de València, 46022 Valencia, SpainCentre for Biomaterials and Tissue Engineering (CBIT), Universitat Politècnica de València, 46022 Valencia, SpainTissue Engineering and Cells Therapy Group (GITTC), School of Medicine, University of Antioquia, Medellin 050010, ColombiaThe aim of this study was to compare the viscoelastic properties of a decellularized mesh from the porcine esophagus, prepared by our group, with two commercial acellular tissues derived from porcine small intestine submucosa and bovine pericardium for use in medical devices. The tissues’ viscoelastic properties were characterized by creep tests in tension, applying the load in the direction of the fibers or the transverse direction, and also by dynamic-shear mechanical tests between parallel plates or in tension at frequencies between 0.1 and 35 Hz. All the tests were performed in triplicate at a constant temperature of 37 °C immersed in distilled water. The tissues’ surface and cross-sectional microstructure were observed by scanning electron microscopy (SEM) to characterize the orientation of the fibers. The matrices of the porcine esophagus present an elastic modulus in the order of 60 MPa when loaded in the longitudinal direction while those of the porcine intestine submucosa and bovine pericardium have an elastic modulus below 5 MPa. Nevertheless, the shear modulus of bovine pericardium nearly triplicates that of the esophageal matrix. The viscoelasticity of decellularized esophageal mucosa is characterized by a fast change in the creep compliance with time. The slope of the creep curve in the double logarithmic plot is twice that of the control samples. These results are consistent with the microstructure observed under electron microscopy regarding the orientation of the fibers that make up the matrices.https://www.mdpi.com/1996-1944/17/1/134decellularized tissueporcine esophageal mucosaviscoelastic propertiescomplianceelastic modulusshear modulus |
| spellingShingle | Sergio Estrada Mira María Inmaculada García-Briega José Luis Gómez Ribelles Luz M. Restrepo Munera Viscoelastic Properties of Acellular Matrices of Porcine Esophageal Mucosa and Comparison with Acellular Matrices of Porcine Small Intestine Submucosa and Bovine Pericardium Materials decellularized tissue porcine esophageal mucosa viscoelastic properties compliance elastic modulus shear modulus |
| title | Viscoelastic Properties of Acellular Matrices of Porcine Esophageal Mucosa and Comparison with Acellular Matrices of Porcine Small Intestine Submucosa and Bovine Pericardium |
| title_full | Viscoelastic Properties of Acellular Matrices of Porcine Esophageal Mucosa and Comparison with Acellular Matrices of Porcine Small Intestine Submucosa and Bovine Pericardium |
| title_fullStr | Viscoelastic Properties of Acellular Matrices of Porcine Esophageal Mucosa and Comparison with Acellular Matrices of Porcine Small Intestine Submucosa and Bovine Pericardium |
| title_full_unstemmed | Viscoelastic Properties of Acellular Matrices of Porcine Esophageal Mucosa and Comparison with Acellular Matrices of Porcine Small Intestine Submucosa and Bovine Pericardium |
| title_short | Viscoelastic Properties of Acellular Matrices of Porcine Esophageal Mucosa and Comparison with Acellular Matrices of Porcine Small Intestine Submucosa and Bovine Pericardium |
| title_sort | viscoelastic properties of acellular matrices of porcine esophageal mucosa and comparison with acellular matrices of porcine small intestine submucosa and bovine pericardium |
| topic | decellularized tissue porcine esophageal mucosa viscoelastic properties compliance elastic modulus shear modulus |
| url | https://www.mdpi.com/1996-1944/17/1/134 |
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