A New Conditionally Immortalized Nile Tilapia (<em>Oreochromis niloticus</em>) Heart Cell Line: Establishment and Functional Characterization as a Promising Tool for Tilapia Myocarditis Studies
A new cell line named the tilapia heart cell line (TAH-11) was established from the heart of tilapia (<i>Oreochromis niloticus</i>) by enzymatic digestion and mechanical separation. The TAH-11 cell line has been stably subcultured for over 80 generations and resuscitated after being froz...
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MDPI AG
2023-03-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/8/3/167 |
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| author | Yanghui Chen Yuan Li Dongneng Jiang Defeng Zhang Yu Huang Jia Cai Jichang Jian Bei Wang |
| author_facet | Yanghui Chen Yuan Li Dongneng Jiang Defeng Zhang Yu Huang Jia Cai Jichang Jian Bei Wang |
| author_sort | Yanghui Chen |
| collection | DOAJ |
| description | A new cell line named the tilapia heart cell line (TAH-11) was established from the heart of tilapia (<i>Oreochromis niloticus</i>) by enzymatic digestion and mechanical separation. The TAH-11 cell line has been stably subcultured for over 80 generations and resuscitated after being frozen in liquid nitrogen for six months, with exuberant cell growth. The optimal culture condition of TAH-11 is L-15 medium supplemented with 10% fetal bovine serum at 25 °C. Sequencing of the mitochondrial 18S rRNAs confirmed that TAH-11 cells were derived from the tilapia. TAH-11 was also identified as a myocardial cell line based on the mRNA expression of the troponin I, α-actin and myoglobin genes. Simultaneously, TAH-11 could be successfully transfected with the GFP reporter gene, suggesting that the TAH-11 cell line could be used for exogenous gene expression in vitro. The TAH-11 cells showed susceptibility to CGSIV, which was demonstrated by the presence of a severe cytopathic effect, suggesting that the TAH-11 cell line is an ideal tool for studying host–virus interaction and potential vaccines. In addition, the expression of inflammatory factors in TAH-11 cells can be remarkably induced following <i>Streptococcus agalactiae</i> or <i>Streptococcus iniae</i>. The present data lays a foundation to further explore the mechanism of how <i>Streptococcus</i> spp. causes tilapia myocarditis. |
| first_indexed | 2024-03-11T06:33:21Z |
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| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-03-11T06:33:21Z |
| publishDate | 2023-03-01 |
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| spelling | doaj.art-cefa8e5a8c3d4a02b2434986050321fb2023-11-17T11:04:31ZengMDPI AGFishes2410-38882023-03-018316710.3390/fishes8030167A New Conditionally Immortalized Nile Tilapia (<em>Oreochromis niloticus</em>) Heart Cell Line: Establishment and Functional Characterization as a Promising Tool for Tilapia Myocarditis StudiesYanghui Chen0Yuan Li1Dongneng Jiang2Defeng Zhang3Yu Huang4Jia Cai5Jichang Jian6Bei Wang7Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Fisheries College of Guangdong Ocean University, Zhanjiang 524088, ChinaHenry Fok College of Biology and Agriculture, Shaoguan University, Shaoguan 512005, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Fisheries College of Guangdong Ocean University, Zhanjiang 524088, ChinaLaboratory of Fishery Drug Development, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Guangzhou 510380, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Fisheries College of Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Fisheries College of Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Fisheries College of Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Fisheries College of Guangdong Ocean University, Zhanjiang 524088, ChinaA new cell line named the tilapia heart cell line (TAH-11) was established from the heart of tilapia (<i>Oreochromis niloticus</i>) by enzymatic digestion and mechanical separation. The TAH-11 cell line has been stably subcultured for over 80 generations and resuscitated after being frozen in liquid nitrogen for six months, with exuberant cell growth. The optimal culture condition of TAH-11 is L-15 medium supplemented with 10% fetal bovine serum at 25 °C. Sequencing of the mitochondrial 18S rRNAs confirmed that TAH-11 cells were derived from the tilapia. TAH-11 was also identified as a myocardial cell line based on the mRNA expression of the troponin I, α-actin and myoglobin genes. Simultaneously, TAH-11 could be successfully transfected with the GFP reporter gene, suggesting that the TAH-11 cell line could be used for exogenous gene expression in vitro. The TAH-11 cells showed susceptibility to CGSIV, which was demonstrated by the presence of a severe cytopathic effect, suggesting that the TAH-11 cell line is an ideal tool for studying host–virus interaction and potential vaccines. In addition, the expression of inflammatory factors in TAH-11 cells can be remarkably induced following <i>Streptococcus agalactiae</i> or <i>Streptococcus iniae</i>. The present data lays a foundation to further explore the mechanism of how <i>Streptococcus</i> spp. causes tilapia myocarditis.https://www.mdpi.com/2410-3888/8/3/167<i>Oreochromis niloticus</i>cell linefish virologymyocardial inflammation |
| spellingShingle | Yanghui Chen Yuan Li Dongneng Jiang Defeng Zhang Yu Huang Jia Cai Jichang Jian Bei Wang A New Conditionally Immortalized Nile Tilapia (<em>Oreochromis niloticus</em>) Heart Cell Line: Establishment and Functional Characterization as a Promising Tool for Tilapia Myocarditis Studies Fishes <i>Oreochromis niloticus</i> cell line fish virology myocardial inflammation |
| title | A New Conditionally Immortalized Nile Tilapia (<em>Oreochromis niloticus</em>) Heart Cell Line: Establishment and Functional Characterization as a Promising Tool for Tilapia Myocarditis Studies |
| title_full | A New Conditionally Immortalized Nile Tilapia (<em>Oreochromis niloticus</em>) Heart Cell Line: Establishment and Functional Characterization as a Promising Tool for Tilapia Myocarditis Studies |
| title_fullStr | A New Conditionally Immortalized Nile Tilapia (<em>Oreochromis niloticus</em>) Heart Cell Line: Establishment and Functional Characterization as a Promising Tool for Tilapia Myocarditis Studies |
| title_full_unstemmed | A New Conditionally Immortalized Nile Tilapia (<em>Oreochromis niloticus</em>) Heart Cell Line: Establishment and Functional Characterization as a Promising Tool for Tilapia Myocarditis Studies |
| title_short | A New Conditionally Immortalized Nile Tilapia (<em>Oreochromis niloticus</em>) Heart Cell Line: Establishment and Functional Characterization as a Promising Tool for Tilapia Myocarditis Studies |
| title_sort | new conditionally immortalized nile tilapia em oreochromis niloticus em heart cell line establishment and functional characterization as a promising tool for tilapia myocarditis studies |
| topic | <i>Oreochromis niloticus</i> cell line fish virology myocardial inflammation |
| url | https://www.mdpi.com/2410-3888/8/3/167 |
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